茶饼病菌侵染对茶树挥发性物质的影响

doi:10.13305/j.cnki.jts.2012.04.009

Abstract

Abstract: The aim of this experiment was to analyze the variation of component and relative amount of volatile compounds from tea plant infested by Exobasidium vexans Masseeon (E. vexans). The volatile compounds released from tea plant (Camellia sinensis var. assamica) healthy and diseased were analyzed by SDE/GC/MS. Result showed that a total of 37 volatile compounds were detected from the healthy tea plant, as 80.195% of total compounds identified from the healthy tea plants. A total of 56 volatile compounds, 92.166% of compounds were identified from the infected plants. However, 13 volatile components which was existed in intact tea leaf were not detected and 31 new volatile compounds were in E. vexans infested plants. Linalool was the main volatile compound in intacted Camellia sinensis var. assamica. The kinds of azotic, sulfur, terpenes, aromatic, green leaf volatile were main volatile compounds in tea plants infested by E. vexans.

Key words: Exobasidium vexans massee, infested, volatile compounds, GC/MS

CLC Number:

ZHANG Chun-hua, SHAN Zhi-guo, WEI Zhao-xia, LI Jia-hua, TANG Jia-yi. Effects of Exobasidium vexans Massee Infection on Volatile Components in Tea Plant (Camellia Sinensis var. assamica)[J]. Journal of Tea Science, 2012, 32(4): 331-340.

References

[1] Pettersson J, Karunaratne S, Ahmed E, et al. The cowpea aphid, shape Aphis craccivora, host plant odours and pheromones[J]. Entomologia Experimentalis et Applicata, 1998, 88(2): 177-184.
[2] Bruin J, Dicke M.Chemical information transfer between wounded and unwounded plants: backing up the future[J]. Biochemical Systematics and Ecology, 2001, 29(10): 1103-1113.
[3] 刘守安, 王梦馨, 韩宝瑜. 植物挥发性物质在茶树病害监测和防御中的作用研究现状[J]. 中国茶叶, 2010, 32(1): 12-14.
[4] 何培青, 柳海燕, 郝林华, 等. 植物挥发性物质与植物抗病防御反应[J]. 植物生理学通讯, 2005, 41(1): 105-110.
[5] De Moraes CM, Lewis WJP, Pare W, et al. Herbivore-in-fested plants selectively attract parasitoids[J]. Nature, 1998, 393: 570-573.
[6] Hamilton-Kemp TR, McCracken CT, Loughrin JH, et al. Effects of some natural volatile compounds on the patho-genic fungi Alternaria alternata and Botrvtis cinerea[J]. ChemEcol, 1992, 18(7): 1083-1086.
[7] Obara N, Hasegawa M, Kodama O.Induced volatiles in elici-tor-treated and rice blast fungus-inoculated rice leaves[J]. Biosci Biotechnol Biochem, 2002, 66(12): 2549-2559.
[8] Arimura G, Kost C, Boland W.Herbivore-induced, indirect plant defences[J]. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids, 2005, 1734(2): 91-111.
[9] Frost C J, Appel H M, Carlson J E, et al. Within-plant signalling via volatiles overcomes vascular constraints on systemic signalling and primes responses against herbivores[J]. Ecology Letters, 2007, 10(6): 490-498.
[10] Farmer E E.Surface-to-air signals[J]. Nature, 2001, 411(6839): 854-856.
[11] 郝树芹, 刘世琦, 张自坤. 银叶病对西葫芦叶片生理生化物质及挥发性物质的影响[J]. 西北农业学报, 2010, 19(10): l4l-l45.
[12] RYU CM, FARAG MA, HU CH, et al. Bacterial volatiles promote growth in Arabidopsis[J]. Proc Natl Acad Sci USA, 2003, 100(14): 4927-4932.
[13] RYU CM, FARAG MA, HU CH, et al. Bacterial volatiles induce syste-mic resistance in Arabidopsis[J]. Plant Physiol, 2005(134): 1017-1026.
[14] FARAG MA, RYU CM, SUMNER LW, et al. GC-MS SPME profi-ling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants[J]. Phytochemistry, 2006, 67(20): 2262-2268.
[15] BHASKAR C, ANITA P, LOK MS.Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro[J]. Mycological Research, 2005(160): 75-78.
[16] 陈雪芬. 一个世纪来的茶饼病[J]. 国外农学—茶叶, 1984(1): 1-6.
[17] 卢凯等. 虫害诱导的水稻挥发物抑制水稻病原菌的生长[J]. 科学通报, 2010, 55(30): 2925-2930.
[18] Zhang Z Z, Li Y, Qi Li, et a1. Antifungal activity of major tea leaf volatile constituents toward Colletorichum camelliae Massea[J]. J Agric Food Chem, 2006 (54): 3936-3940.
[19] 江楚平, 杜仲福, 刘世贤, 等. 茶饼病菌的侵染及其生物学特性[J]. 四川农业大学学报, 1985, 3(2): 9-l5.
[20] DickeM, Takabayashi J, PosthumusM A, et al. Plant-phytoseiid interactionsmediated by herbivore induced plantvolatiles:variation in production of cues and in responses of predatorymites[J]. ExpApplAcaro, 1998, 22: 311-333.
[21] Hampden J.Chang in cotton leaf chemistry induced by volatile elicitors[J]. Phytochem, 1987, 26: 1357-1360.
[22] Croft K, Juttner F, Slusarenko A J.Volatile products of the lipoxygenase pathway evolved from Phaseolus vulgaris (L.) leaves inoculated with Pseudomonas syringae pv phaseolicola[J]. Plant Physiol, 1993, 101: 13-24.
[23] Prost I, Dhondt S, Rothe G, et a1.Evaluation of the antimicrobial activities of plant oxylipins suppprts their involvement in defense against pathogens[J]. Plant Physiol, 2005, 139: 1902-1913.
[24] Shulaev V, Silverman P, Raskin I.Airborne signaling by methyl salicylate in plant pathogen resistance[J]. Nature, 1997, 385(6618): 718-721.
[25] 何红卫, 廖令洁, 肖文娟, 等. 茉莉酸甲酯对烟草幼苗抗病毒的影响[J]. 热带亚热带植物学报, 2004, 13: 241-246.
[26] Bate N J, Rothstein S J.C6-volatiles derived from lipoxygenase pathway induce a subset of defenserelaed genes[J]. The Plant Journal, 1998, 16: 561-569.
[27] AttiehJ, DjianaR, KoonjulP, et a1. Cloning and functional expression of two plant thiol methyltransferase sa new class of enzymes involve din the biosynthesis of sulfur volatiles[J]. PlantMolBiol, 2002, 50(3): 511-521.
[28] Shiojiri K, Kishimoto K, Ozawa R, et al. Changing green leaf volatile biosynthesis in plants: An approach for improving plant resistance against both herbivores and pathogens[J]. Proc Natl Acad Sci USA, 2006, 103: 16672-16676.
[29] Yasmin J, Cardoza, Alborn H T, et al. In vito volatile emission from peanut plants induced by simultaneous fungal infection and insect damage[J]. Chem Ecol, 2002, 28: 161-174.
[30] Croft KPC, Voisey CR, Slusarenko A J.Mechanism of hypersensitive cell collapse: Correlation of increased lipoxygenaseactivity with membrane damage in leaves of Phaseolus vulgaris inoculated with an avirulent race of pseudomonas syringeapv. Phaseolicola[J]. Physiol Mol Plant, 1990, 36: 49-62.
[31] Kaori S, Kyutaro K, Rika O, et a1. Changing green leaf volatile biosynthesis in plants:An approach for improving plant resistance against both herbivores and pathogens[J]. Proc Natl Acad Sci USA,2006, 45(103): l6672-16676.
[32] Zeringue H J, Brown R L, Neucere J N, et al. Relationships between C-6-C-12 alkanal and alkenal volatile contents and resistance of maize genotypes to Aspergillus flavus and aflatoxin production[J]. Journal of agricultural and food chemistry, 1996, 44: 403-407.
[33] Vaughn S F, Gardner H W.Lip—derived aldehydes inhibitfungipathogeniconsoybean[J]. Chem Ecol, 1993, l9(10): 2337-2345.

Effects of Exobasidium vexans Massee Infection on Volatile Components in Tea Plant (Camellia Sinensis var. assamica)

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 10

[1]	WANG Chen, LÜ Shidong, LIAN Ming, MENG Qingxiong. Analysis of Aroma Components in Big-leaf Tea Plant (Camellia sinensis var. assamica) Flower Using Headspace Solid-phase Microextraction Coupled with GC/MS [J]. Journal of Tea Science, 2016, 36(2): 175-183.
[2]	BAO Zhongzan, DONG Rongjian, DENG Zhaopu, XIE Bingtao. The Research on Method of Identifying New Green Tea and Stale Green Tea Based on Characteristic Aromatic Components of Stale Tea Aroma [J]. Journal of Tea Science, 2015, 35(6): 583-588.
[3]	ZHOU Chunjuan, ZHUANG Donghong, GUO Shoujun, ZHU Hui, MA Ruijun, WU Qinghan. Classification and Identification of Different Aromatics in Tea Made from Different Cultivar of Fenghuang Dancong [J]. Journal of Tea Science, 2014, 34(6): 609-616.
[4]	DOU Hong-liang, LI Chun-mei, GU Hai-feng, HAO Ju-fang. Comparative Analysis on Aromatic Components of Green Tea and Fresh Green Tea Beverage Using HS-SPME/GC-MS/GC-Olfactometry/RI Methods [J]. Journal of Tea Science, 2007, 27(1): 51-60.
[5]	LU Xiao, REN Feng-shan, WANG Wen-bo, DU Hong-xia, LI Hui-dong, DING Rui-yan, ZHU Duan-wei, LEE Nanju A, SHEILD Ross, KENNEDY Ivan R. Determination of Endosulfan and Endosulfan Sulfate in Tea by ELISA [J]. Journal of Tea Science, 2006, 26(2): 141-146.