EGCG甲基化衍生物酶促合成的反应条件研究

doi:10.13305/j.cnki.jts.2012.05.001

Abstract

Abstract: Taken the yields of EGCG3"Me, EGCG4′′Me and EGCG3′Me as the main index, the investigation on the reaction condition of EGCG methylated derivatives produced from EGCG catalyzed by EGCG-O-Methyltransferase were conducted. Results showed that the optimum reaction condition was as follows: the concentration of DTT and Mg²⁺ was 2mmol/L, the initial pH was 7.5 and the reaction temperature was 35℃. Under the above conditions, the yields of EGCG3"Me, EGCG4′′Me and EGCG3′Me could reach 386.39μg/mL, 23.40μg/mL and 107.01μg/mL, respectively.

Key words: EGCG methylated derivatives, enzymatic synthesis, EGCG-O-Methyltransferase

CLC Number:

LV Hai-peng, FEI Dong-mei, ZHANG Yue, LIN Zhi, PENG Qun-hua, GUO Li, TAN Jun-feng. Study on the Reaction Condition of EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase[J]. Journal of Tea Science, 2012, 32(5): 441-447.

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Study on the Reaction Condition of EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase

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