茶悬浮培养细胞玻璃化超低温保存研究

doi:10.13305/j.cnki.jts.2009.2.006

Abstract

Abstract: To preserve eminent cell lines with stable metabolism, the cryopreservation of suspension culture cells by vitrification in Camellia sinensis has been preliminarily investigated in this article. It showed that the preculture for four days was best, loading with 60% PVS₂ in freezing bath for 20 minutes was optimal. Treating with 100% PVS₂ dehydration in freezing bath for 60 minutes was suggested. The cryopreservation of suspension culture cells warmed in 40℃water bath had the highest survival rate. The cells’ survival rate was as high as 76% when these parameters were adopted through the whole trial. The procedure of cryopreservation of suspension culture cells by vitrification in Camellia sinensis was preliminarily established.

Key words: tea(Camellia sinensis), suspension culture cell, vitrification, cryoproservation

CLC Number:

TS272
Q942

LIU Ya-jun, GAO Li-ping, XIA Tao, GAO Ke-jun. Study on Cryopreservation of Suspension Culture Cells by Vitrification in Camellia Sinensis[J]. Journal of Tea Science, 2009, 29(2): 120-126.

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Study on Cryopreservation of Suspension Culture Cells by Vitrification in Camellia Sinensis

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