茶树蛋白质双向电泳样品制备技术研究

doi:10.13305/j.cnki.jts.2011.03.014

Abstract

Abstract: In order to develop an efficient protein extraction method suitable for two-dimensional electrophoresis from tea plant, three methods (TCA/acetone precipitation, improved Tris-HCl extraction and phenol-methanol/ammonium acetate precipitation) for total protein extraction in plant were compared. The result showed that the improved Tris-HCl extraction was the best method suitable for tea leaf protein sample preparation in these three techniques and the extracts were separated by two-dimensional electrophoresis. Consequently, 1117±9.89 protein spots were found and their isoelectric points were found to lie between pI5~7. The relative molecular weight of proteins was between 15.00~95.00kD. The improved Tris-HCl extraction could get the highest resolution of two dimensional electrophoresis and high yield of protein, and it could meet the requirement for the research on tea proteomecis.

Key words: Camellia sinensis (L.) O.Kuntze, two dimensional electrophoresis, protein sample preparation, proteomics

CLC Number:

S571.1
Q503

LI Qin, HUANG Jian-an, LIU Shuo-qian, LI Juan, LIU Zhong-hua. Protein Extraction Method Suitable for Tea Plant[J]. Journal of Tea Science, 2011, 31(3): 173-178.

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Protein Extraction Method Suitable for Tea Plant

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