传统SMB、Varicol和Partial-discard工艺分离纯化ECG和EGCG的比较研究

doi:10.13305/j.cnki.jts.2011.03.007

Abstract

Abstract: With C₁₈ bonded silica as stationary phase, ethanol and water (20︰80, V/V) as mobile phase, tea polyphenols were separated by traditional Simulated Moving Bed, Varicol process and Partial-discard process. Column configuration, switching time, feed flow rate, elution flow rate were optimized respectively in order to obtain epicatechin gallate (ECG) and epigallocatechin gallate(EGCG)with high purity and high recovery. Varicol process could be employed to separate 98% green tea polyphenols (TP98) to obtain ECG with 91.33% purity and 91.41% recovery. Partial-discard process could be adopted to separate TP98 to obtain EGCG with 90.12% purity and 97.83% recovery. Compared with traditional process, Varicol process has the advantages of higher purity and recovery of target product, while Partial-discard process shows the advantages of increasing the concentration of raffinate and the purity of target product.

Key words: simulated moving bed, EGCG, ECG, varicol, partial-discard

CLC Number:

TS272.4

HUANG Yong-dong, JIANG He-yuan, JIANG Yong-wen, ZHANG Jian-yong, WANG Bin, BAI Yan, WEN Zhi-hao, WANG Wei-wei. Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography[J]. Journal of Tea Science, 2011, 31(3): 201-210.

References

[1] 林炳昌. 模拟移动床色谱技术[M]. 北京: 化学工业出版社, 2008: 1.
[2] Broughton D B, Gerhold C G. Continuous sorption process employing fixed bed of sorbent and moving inlets and outlets: US, 2985589[P].1961-5-23.
[3] Universal Oil Products Co. Understanding the SorbexTM Process [EB/OL]. [2010-08-02]. http://www.uop.com/.
[4] Long N V D, Le T H, Kim J I, et al. Separation of D-psicose and D-fructose using simulated moving bed chromatography[J]. Journal of Separation Science, 2009, 32(11): 1987-1995.
[5] Makart S, Bechtold M, Panke S.Separation of amino acids by simulated moving bed under solvent constrained conditions for the integration of continuous chromatography and biotransformation[J]. Chemical Engineering Science, 2008, 63(21): 5347-5355.
[6] Rajendranb A, Paredesa G, Mazzotti M.Simulated moving bed chromatography for the separation of enantiomers[J]. Journal of Chromatography A, 2009, 1216(4): 709-738.
[7] Juza M, Mazzotti M, Morbidelli M.Simulated moving-bed chromatography and its application to chirotechnology[J]. Trends in Biotechnology, 2000, 18(3): 108-118.
[8] Kaiser P, Ottolina G, Carrea G, et al. Preparative-scale separation by simulated moving bed chromatography of biocatalytically produced regioisomeric lactones[J]. New Biotechnology, 2009, 25(4): 220-225.
[9] Wei F, Zhao Y X.Separation of capsaicin from capsaicinoids by simulated moving bed chromatography[J]. Journal of Chromatography A, 2008, 1187(1/2): 281-284.
[10] 赵雪飞, 高丽娟, 赖仕全. 模拟移动床色谱法分离C60的条件选择[J]. 辽宁科技大学学报, 2008, 31(3/4): 225-228.
[11] Olivier L H, Marc N R. Process and device for separation with variable-length chromatographic zones: US, 6712973[P].2004-03-30.
[12] Zhang Z Y, Mazzotti M, Morbidelli M.PowerFeed operation of simulated moving bed units: changing flow-rates during the switching interval[J]. Journal of Chromatography A, 2003, 1006(1/2): 87-99.
[13] Kawajiri Y, Biegler L T.Optimization strategies for simulated moving bed and PowerFeed processes[J]. American Institute of Chemical Engineers Journal, 2007, 52(4): 1343-1350.
[14] Schramm H, Kaspereit M, Kienle A, et al. Simulated moving bed process with cyclic modulation of the feed concentration[J]. Journal of Chromatography A. 2003, 1006(1/2): 77-86.
[15] Schramm H, Kaspereit M, Kienle A, et al. Improving simulated moving bed processes by cyclic modulation of the feed concentration[J]. Chem Eng Technol, 2002, 25(12): 1151-1155.
[16] Keßler L C, Seidel-Morgenstern A.Improving performance of simulated moving bed chromatography by fractionation and feed-back of outlet streams[J]. Journal of Chromatography A, 2008, 1207(1/2): 55-71.
[17] Migliorini C, Wendlinger M, Mazzotti M.Temperature gradient operation of a simulated moving bed unit[J]. Ind Eng Chem Res, 2001, 40(12): 2606-2617.
[18] Mun S Y, Wang N H L. Optimization of productivity in solvent gradient simulated moving bed for paclitaxel purification[J]. Process Biochemistry, 2008, 43(12): 1407-1418.
[19] Mun S Y.Enhanced Separation Performance of a Five-Zone Simulated Moving Bed Process by Using Partial Collection Strategy Based on Alternate Opening and Closing of a Product Port[J]. Industrial & Engineering Chemistry Research, 2010, 49(19): 9258-9270.
[20] Paredes G, Abel S, Mazzotti M, et al. Analysis of a Simulated Moving Bed Operation for Three-Fraction Separations (3F-SMB)[J]. Ind Eng Chem Res, 2004, 43(19): 6157-6167.
[21] 钟世安, 周春山, 杨娟玉. 高效液相色谱法分离纯化酯型儿茶素的研究[J]. 化学世界, 2003, 44(5): 237-239, 245, 249.
[22] 张莹, 施兆鹏, 聂洪勇, 等. 制备型逆流色谱分离绿茶提取物中儿茶素单体[J]. 湖南农业大学学报, 2003, 29(5): 408-411, 417.
[23] 姜绍通, 黄静, 潘丽军, 等. 儿茶素单体的分离纯化方法: 中国, 1603319[P], 2005-04-06.
[24] 蔡宇杰, 丁彦蕊, 张大兵, 等. 模拟移动床色谱技术及其应用[J]. 色谱, 2004, 22(2): 111-115.
[25] 张家元, 林炳昌. 三带模拟移动床色谱的实验研究[J]. 鞍山钢铁学院学报, 1999(3): 144-148.
[26] Ludemann-Hombourger O, Nicoud, R M, Bailly M. The Varicol Process: A New Multicolumn Continuous Chromatographic Process[J]. Sep Sci Technol, 2000, 35(12): 1829-1862.
[27] Zhang Z Y, Hidajat K, Ray A K, et al. Multiobjective Optimization of SMB and Varicol Process for Chiral Separation[J]. AIChE Journal, 2002, 48(12): 2800-2816.
[28] Toumi A, Engell S, Ludemann-Hombourger O, et al. Optimization of simulated moving bed and Varicol processes[J]. Journal of Chromatography A, 2003, 1006(1/2): 15-31.
[29] Zhang Z Y, Mazzotti M, Morbidelli M.Continuous Chromatographic Processes with a Small Number of Columns: Comparison of Simulated Moving Bed with Varicol, PowerFeed, and ModiCon[J]. Korean J Chem Eng, 2004, 21(2): 454-464.
[30] Zhang Y, Hidajat K, Ray A K.Multi-objective optimization of simulated moving bed and Varicol processes for enantio-separation of racemic pindolol[J]. Separation and Purification Technology, 2009, 65(3): 311-321.
[31] Bae Y S, Lee C H.Partial-discard strategy for obtaining high purity products using simulated moving bed chromatography[J]. Journal of Chromatography A, 2006, 1122(1/2): 161-173.
[32] Keßler L C, Seidel-Morgenstern A.Improving performance of simulated moving bed chromatography by fractionation and feed-back of outlet streams[J]. Journal of Chromatography A, 2008, 1207(1/2): 55-71.
[33] Ludemann-hombourger Olivier, Nicoud Roger Marc. Process and device for separation with variable-length chromatographic zones: US, 6712973[P].2004-03-30.
[34] Yan Zhang, Kus Hidajat, Ajay K Ray.Enantio-separation of racemic pindolol on α1-acid glycoprotein chiral stationary phase by SMB and Varicol[J]. Chemical Engineering Science. 2007, 62(5): 1364-1375.

Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	CHEN Ke, WANG Yuanzhu, YANG Xiaoying, ZHANG Dongying, ZHU Qiangqiang. Preparation of Nanoparticules with Chitosan Complexed β-lactoglobulin Loaded EGCG and their Effects on Blood Glucose in Diabetic Mice [J]. Journal of Tea Science, 2022, 42(5): 731-739.
[2]	YU Rongxin, ZHENG Qinqin, CHEN Hongping, ZHANG Jinsong, ZHANG Xiangchun. Recent Advances in Catechin Biomedical Nanomaterials [J]. Journal of Tea Science, 2022, 42(4): 447-462.
[3]	ZHANG Yini, JI Zheng. Econometric Analyses of EGCG Research Literature [J]. Journal of Tea Science, 2022, 42(3): 423-434.
[4]	CHEN Chunxiao, LOU Wenyu, DING Zhenjian, LI Zhuoye, YANG Yuanyuan, JIN Peng, DU Qizhen. Vardenafil Improves the Proliferative Inhibition of EGCG-β-lactoglobulin Nanoparticles Against Liver Cancer Cells [J]. Journal of Tea Science, 2020, 40(4): 528-535.
[5]	XU Yan, CAI Xiaqiang, XIE Qianjin, TAI Lingling, LIU Zenghui. The Intergative Effects of Epigallocatechin-3-gallate and Vitamin C on Serum Uric Acid Levels in Hyperuricemic Mice [J]. Journal of Tea Science, 2020, 40(3): 407-414.
[6]	ZHANG Jianyong, CHEN Lin, CUI Hongchun, WANG Weiwei, XUE Jinjin, XIONG Chunhua, JIANG Heyuan. Optimization of Technical Parameters for Chemical Synthesis of Theasinensin A by PBD and RSM [J]. Journal of Tea Science, 2020, 40(1): 51-62.
[7]	FANG Hongfeng, ZHANG Huixia, WANG Guohong, YANG Minhe. Fungal Mixed Fermentation for The Production of Lipase and Its Activity Analysis in Galloylated Catechin Hydrolysis [J]. Journal of Tea Science, 2019, 39(1): 88-97.
[8]	SUN Lili, ZENG Xiangquan, Nilesh W Gaikwad, WANG Huan, XU Hairong, YE Jianhui. Determination of Green Tea Catechin Biomarkers and It′s Relative Application [J]. Journal of Tea Science, 2017, 37(5): 429-441.
[9]	HUANG Xiangxiang, YANG Zhe, YU Lijun. Research Progress of Green Tea and EGCG for the Prevention and Mitigation of Chronic Obstructive Pulmonary Disease Caused by Cigarette Smoke [J]. Journal of Tea Science, 2017, 37(4): 332-338.
[10]	ZHANG Jing, HUANG Jian'an, CAI Shuxian, YI Xiaoqin, LIU Jianjun, WANG Yingzi, TIAN Lili, LIU Zhonghua. Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ₄₂ in vivo and in vitro [J]. Journal of Tea Science, 2016, 36(6): 655-662.
[11]	LIU Min, RAO Guowu, HUA Yunfen. Research Advance in Synthesis and Pharmacological Effects of EGCG Derivatives [J]. Journal of Tea Science, 2016, 36(2): 119-130.
[12]	LIU Hongguo. The Investigation of the Protection Effects and Mechanism of EGCG on Kidney Ischemia Reperfusion Injury [J]. Journal of Tea Science, 2016, 36(2): 169-174.
[13]	YE Xiaofeng, ZHANG Fang, ZHU Junli, ZHANG Lei, XIE Dumei. Inhibition of Biofilm Development and Spoilage Potential in Shewanella baltica by Epigallocatechin Gallate [J]. Journal of Tea Science, 2016, 36(2): 201-209.
[14]	HUANG Meirong, YING Hao, JIANG Yongwen, JIANG Heyuan, DU Qizhen. Research on Preparation and Antitumor Activity of EGCG Naonparticles [J]. Journal of Tea Science, 2015, 35(6): 605-612.
[15]	MENG Qing, TU Youying. Study on Absorption of Theaflavin and EGCG in Caco-2 Cell Model [J]. Journal of Tea Science, 2015, 35(3): 233-238.