EGCG-O-甲基转移酶(EOMT)在重组大肠杆菌中的表达条件研究

doi:10.13305/j.cnki.jts.2011.04.001

Abstract

Abstract: EGCG3"Me could be produced from EGCG catalyzed by EGCG-O-Methyltransferase. Taken the yield of EGCG3"Me as main index, the present study focused on the producing conditions of EGCG-O-Methyltransferase induced by IPTG in recombinant E. coli bacteria. Results showed that the optimum producing conditions were as follows: the concentration of IPTG was 0.05mmol/L, the induction time was 20h, the initial pH of medium was 7.0 and the induction temperature was 20℃.

Key words: EGCG-O-Methyltransferase, recombinant Escherichia coli, expression conditions

CLC Number:

FEI Dong-mei, LIN Zhi, LU Hai-peng, ZHANG Yue, TAN Jun-feng, GUO Li. Study on Expression Conditions of EGCG-O-Methyltransferase in Recombinant Escherichia coli Bacteria[J]. Journal of Tea Science, 2011, 31(4): 333-340.

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Study on Expression Conditions of EGCG-O-Methyltransferase in Recombinant Escherichia coli Bacteria

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