茶树品种“玉绿”和“玉笋”父本的EST-SSR标记鉴定

doi:10.13305/j.cnki.jts.2009.6.05

Abstract

Abstract: EST-SSR primers were used to identify the male parents for Yulv and Yusun among Yabukita seeding and other 4 possible male parents. Totally 34 alleles were amplified using 16 core EST-SSR primers, the number of alleles per primer ranged from 1 to 3, on average of 2.12. The percentage of common alleles with Yulv is 72.22%, 44.44%, 55.56%, 72.22%, 61.11% each other, the maximum is Yabukita seeding and Xiangbolv, the minimum is Fuding Dabaicha, The percentage of Yusun is 73.91%, 43.48%, 52.17%, 60.87% and 47.83%, respectively, the maximum is Yabukita seeding, the minimum is Fuding Dabaicha.The similarity coefficient among different cultivars varied from 0.19 to 0.85, on average of 0.52. The 8 accessions were classified into 4 groups based on the UPGMA method with the similarity coefficient at 0.61: The first group is Yabukita, Yabukita seeding and Yusun. The second is Longjing 43. The third is Xiangbolv, Yulv and Zhuyeqi. The last is Fuding Dabaicha. The results of the similarity coefficient and dendrogram showed that the accessiongs have more genetic diversity and heterosis, Xiangbolv is the possible male parent of Yulv and which of Yusun should be identified furtherly.

Key words: tea, Yulv, Yusun, EST-SSR, male parents identification

CLC Number:

S571.1
Q523

ZHANG Shu-guang, DONG Li-juan, YANG Yang, LIU Zhen, WANG Xu, NING Jing. Identification of Male Parents for “Yulv” and “Yusun” Tea Variety Based on EST-SSR Markers[J]. Journal of Tea Science, 2009, 29(6): 430-435.

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Identification of Male Parents for “Yulv” and “Yusun” Tea Variety Based on EST-SSR Markers

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