应用叶绿素荧光法鉴定茶树品种抗寒性的研究

doi:10.13305/j.cnki.jts.2011.06.014

Abstract

Abstract: Application of chlorophyll fluorescence technique has shown a favorable prospect in the research field for plant resistance. In the present research, referring to the measurement results by electrolyte conductivity method, appropriate chlorophyll fluorescence parameters were studied for the determination of cold resistance of tea plants. The results showed that the cold resistance of different tea cultivars measured by chlorophyll fluorescence parameter Fv/Fm with logistic equation was the same as which were obtained by electrolyte conductivity method: cv.Shuchazao> cv.Wancha91> cv.Pingyangtezao. However, the determination conclusions by Fv/Fo, Fm and Fo were not correspond to the result acquired by electrolyte conductivity method. For the determination of plant resistance, we concluded that chlorophyll fluorescence method is more convenient and less interfered than the traditional electrolyte conductivity method. Therefore, it was treated as a new method to identify the cold resistance of tea plants.

Key words: Camellia Sinensis, chlorophyll fluorescence method, logistic equation, cold resistance

CLC Number:

PANG Lei, ZHOU Xiao-sheng, LI Ye-yun, JIANG Chang-jun. Study and Identification of Cold Resistance of Camellia Sinensis by Chlorophyll Fluorescence Method[J]. Journal of Tea Science, 2011, 31(6): 521-524.

References

[1] 骆颖颖, 梁月荣. 茶树抗寒性的间接鉴定[J]. 茶叶科学技术, 1998(3): 1-6.
[2] 朱根海, 刘祖祺, 朱培仁. 应用Logistic方程确定植物组织低温半致死温度的研究[J]. 南京农业大学学报, 1986(3): 11-16.
[3] 杨华, 唐茜, 黄毅, 等. 用电导法配合Logistic方程鉴定茶树的抗寒性[J]. 福建茶业, 2006(3): 30-32.
[4] 黄海涛, 余继忠, 张伟, 等. 电导法配合Logistic方程鉴定茶树抗寒性的探讨[J]. 浙江农业科学, 2009(3): 577-578.
[5] Smillie R M, Hetherington S E.Stress tolerance and stressinduced injury in crop plants measured by chlorophyll fluorescence in vivo: chilling, freezing, ice cover, heat and high light[J]. Plant Physiology, 1983, 72(4): 1043-1050.
[6] 周蕴薇, 刘艳萍, 戴思兰. 用叶绿素荧光分析技术鉴定植物抗寒性的剖析[J]. 植物生理学通讯, 2006, 42(5): 945-950.
[7] Johannes M A M CLEMENT, Philip R VAN HASSELT. Chlorophyll fluorescence as a parameter for frost hardiness in winter wheat[J]. Phyton,1996, 36(1): 29-41.
[8] Ying J, Lee EA, Tonenaar M.Response of maize leaf photosynthesis to low temperature during the grain-filling period[J]. Field Crops Res, 2000, 68(2): 87-96.
[9] Rose R, Haase D.Chlorophyll fluorescence and Variations in tissue cold hardiness in response to freezing stress in Douglas-fir seedlings[J]. New Forests, 2002, 23(2): 81-96.
[10] 王爱芳, 张钢, 魏士春, 等. 温度与光周期对樟子松实生苗针叶抗寒性的影响[J]. 中国农学通报, 2007, 23(2): 156-161.
[11] 惠红霞, 许兴, 李前荣. 外源甜菜碱对盐胁迫下枸杞光合功能的改善[J]. 西北植物学报, 2003, 23(12): 2137-2142.
[12] 赵丽英, 邓西平, 山仑. 不同水分处理下冬小麦旗叶叶绿素荧光参数的变化研究[J]. 中国生态农业学报, 2007, 15(1): 63-66.
[13] Perks MP, Osborne BA, Mitchell DT.Rapid predictions of cold tolerance in Douglas—fir seedlings using chlorophyll fluorescence after freezing[J]. New Forests, 2004, 28(1): 49-62.
[14] 李国景, 刘永华, 吴晓花, 等. 长豇豆品种耐低温弱光性和叶绿素荧光参数等的关系[J]. 浙江农业学报, 2005, 17(6): 359-362.
[15] 王可玢, 赵福洪, 王孝宣, 等. 用体内叶绿素a荧光动力学鉴定番茄的抗冷性[J]. 植物学通报,1996, 13(2): 29-33.

Study and Identification of Cold Resistance of Camellia Sinensis by Chlorophyll Fluorescence Method

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City [J]. Journal of Tea Science, 2022, 42(5): 601-609.
[2]	ZHOU Hanchen, YANG Jihong, XU Yujie, WU Qiong, LEI Pandeng. Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis [J]. Journal of Tea Science, 2022, 42(5): 638-648.
[3]	XING Anqi, WU Zichen, XU Xiaohan, SUN Yi, WANG Genmei, WANG Yuhua. Research Advances of Fluoride Accumulation Mechanisms in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 301-315.
[4]	WANG Tao, WANG Yiqing, QI Siyu, ZHOU Zhe, CHEN Zhidan, SUN Weijiang. Identification and Transcriptional Regulation of CLH Gene Family and Expression Analysis in Albino Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 331-346.
[5]	LIU Fuhao, FAN Yangen, WANG Yu, MENG Fanyue, ZHANG Lixia. Screening and Identification of Chaperone CsHIPP26.1 Chelating Ions in Tea Cultivar ‘Huangjinya’ [J]. Journal of Tea Science, 2022, 42(2): 179-186.
[6]	WANG Pengjie, YANG Jiangfan, ZHANG Xingtan, YE Naixing. Research Advance of Tea Plant Genome and Sequencing Technologies [J]. Journal of Tea Science, 2021, 41(6): 743-752.
[7]	ZHOU Hanchen, LEI Pandeng. The Functional Identification of Two Alternative Splicing Transcripts of CsNES [J]. Journal of Tea Science, 2021, 41(6): 753-760.
[8]	YAN Minghui, LIU Ke, WANG Man, LYU Ying, ZHANG Qian. Complete Chloroplast Genome of Camellia sinensis cv. Xinyang 10 and Its Phylogenetic Evolution [J]. Journal of Tea Science, 2021, 41(6): 777-788.
[9]	LIN Xinying, WANG Pengjie, CHEN Xuejin, GUO Yongchun, GU Mengya, ZHENG Yucheng, YE Naixing. Identification of LOX Gene Family in Camellia sinensis and Expression Analysis in the Process of White Tea Withering [J]. Journal of Tea Science, 2021, 41(4): 482-496.
[10]	WANG Yanding, WANG Huan, LI Nana, WANG Lu, HAO Xinyuan, WANG Yuchun, DING Changqing, YANG Yajun, WANG Xinchao, QIAN Wenjun. Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis [J]. Journal of Tea Science, 2021, 41(4): 497-510.
[11]	CHEN Siwen, KANG Rui, GUO Zhiyuan, ZHOU Qiongqiong, FENG Jiancan. Cloning and Expression Analysis of CsCML16 in Tea Plants (Camellia sinensis) under Low Temperature Stress [J]. Journal of Tea Science, 2021, 41(3): 315-326.
[12]	ZHAO Yiqing, LIU Zhengjun, ZHANG Tianxin, ZHAO Yanting, XIAO Bin, GAO Yuefang. Cloning of CsCHLI Gene and Its Expression Analysis in Different Albino Tea Cultivars (Camellia sinensis) [J]. Journal of Tea Science, 2021, 41(3): 327-336.
[13]	GUO Lingling, ZHANG Fen, CHENG Hao, WEI Kang, RUAN Li, WU Liyun, WANG Liyuan. Molecular Cloning and Expression Analysis of CsAAPs Gene Subfamily in Camellia Sinensis [J]. Journal of Tea Science, 2020, 40(4): 454-464.
[14]	WANG Minghan, DING Ding, ZHANG Chenyu, GAO Xizhi, CHEN Jianjiao, TANG Han, SHEN Chengwen. Effects of Drought Stress on Growth and Chlorophyll Fluorescence Characteristics of Tea Seedlings [J]. Journal of Tea Science, 2020, 40(4): 478-491.
[15]	GUO Yongchun, CHEN Jinfa, ZHAO Feng, WANG Shuyan, WANG Pengjie, ZHOU Peng, OUYANG Liqun, JIN Shan, YE Naixing. Study on the Distribution of Glyphosate and Its Metabolite Aminomethylphosphonic Acid in Camellia Sinensis [J]. Journal of Tea Science, 2020, 40(4): 510-518.