EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响

doi:10.13305/j.cnki.jts.2006.03.013

Abstract

Abstract: In the present paper, effects of EGCG and interactions of EGCG with Zn²⁺on the growth of androgen-insensitive human prostate cancer cells (PC-3) were investigated by MTT assay. Effects of EGCG and Zn²⁺ on absorption of zinc and cadmium in PC-3 cells were detected by Flame Atomic Absorption Spectrometry (AAS) assay; and also the changes of Zn²⁺ on the partition coefficients of EGCG in n-octanol/PBS or n-octanol / water system were determined by HPLC technique. EGCG was screened for growth inhibition of PC-3 cells in dose- and time-dependent manners. Both Zn²⁺ and EGCG decreased viability of PC-3 cells in dose- and time-dependent relations, and Zn²⁺ ameliorated the effect of EGCG on the growth of PC-3 cells. Also, Zn²⁺ decreased the absorption of PC-3 cells on Cd²⁺, and EGCG could enhance the effect as well.

Key words: catechins, Zn²⁺, Cd²⁺, PC-3 cells

CLC Number:

S571.1
R983

CHEN Xun, YU Hai-ning, SHEN Sheng-rong. Effect of Interactions Between EGCG and Zn²⁺ on the Growth of Prostate Cancer PC-3 Cells[J]. Journal of Tea Science, 2006, 26(3): 219-224.

References 21

[1]	Rebbeck T R.Genetics, disparities, and prostate cancer[J]. LDI Issue Brief 2005, 10(7): 1~4.
[2]	Landis S H, Murray T, Bolden S, et al. Cancer statistics[J]. Cancer J Clin, 1998, 48(6~29): 43~62.
[3]	Nakazato T, Ito K, Miyakawa Y, et al. Catechin, a green tea component, rapidly induces apoptosis of myeloid leukemic cells via modulation of reactive oxygen species production in vitro and inhibits tumor growth in vivo[J]. Haematologica, 2005, 90(3): 317~325.
[4]	Kinlen, L J, Willows A N, Goldblatt P, et al. Tea consumption and cancer[J]. Brit J Cancer, 1988(58): 397~401.
[5]	Heilburn L K, Nomura A, Stemmermann, G N.Black teaconsumption and cancer risk: A prospective study[J]. Br J Cancer, 1986(54): 677~683.
[6]	Wynder E L, Rose D P, Cohen L A.Nutrition and prostate cancer: a proposal for dietary intervention[J]. Nutr Cancer, 1994(22): 1~10.
[7]	Hussain T, Gupta S, Adhami V M, et al. Green tea constituent epigallocatechin-3-gallate selectively inhibits COX-2 without affecting COX-1 expression in human prostate carcinoma cells[J]. Int J Cancer, 2005, 113(4): 660~669.
[8]	Hiipakka R A., Zhang H Z, Dai W, et al. Structure-activity relationships for inhibition of human 5alpha-reductases by polyphenols[J]. Biochem Pharmacol, 2002, 63(6): 1165~1176.
[9]	Brusselmans K, De Schrijver E, Heyns W, et al. Epigallocatechin-3-gallate is a potent natural inhibitor of fatty acid synthase in intact cells and selectively induces apoptosis in prostate cancer cells[J]. Int J Cancer, 2003, 106(6): 856~862.
[10]	Pezzato E., Sartor L, Dell'Aica I, et al. Prostate carcinoma and green tea: PSA-triggered basement membrane degradation and MMP-2 activation are inhibited by (-) epigallocatechin-3-gallate[J]. Int J Cancer, 2004, 112(5): 787-792.
[11]	Vayalil P K, Katiyar S K.Treatment of epigallocatechin-3-gallate inhibits matrix metalloproteinases-2 and -9 via inhibition of activation of mitogen-activated protein kinases, c-jun and NF-kappaB in human prostate carcinoma DU-145 cells[J]. Prostate 2004, 59(1): 33~42.
[12]	Ren F, Zhang S, Mitchell, S H, et al. Tea polyphenols down-regulate the expression of the androgen receptor in LNCaP prostate cancer cells[J]. Oncogene, 2000, 19(15): 1924~32.
[13]	Ishii,K., Otsuka T., Iguchi K., et al. Evidence that the prostate-specific antigen (PSA)/Zn2+ axis may play a role in human prostate cancer cell invasion[J]. Cancer Lett,2004, 207(1):79~87.
[14]	Scudiero D A, Shoemarker R H, Paull K D, et al. Evaluation of a soluble tetrazolium/formaza assay for cell growth and drug sensitivity in culture using human and other tumor cell lines[J]. Cancer Res, 1998(48): 4827.
[15]	Ayhan D, Erol P, Fethiye G, et al. Adsorption of Cu(Ⅱ), Zn, 2005(282): 20~25.
[16]	Liang Y R.Analysis of theaflavins by HPLC-photodiode arraydetector. Acta[J]. Agriculture.Universitati 1992, 18(s): 70~74.
[17]	Tang D S, Shen S R, Chen X., et al. Interaction of catechins with aluminum in vitro[J]. J Zhejiang Univ Sci 2004, 5(6): 668~675.
[18]	K Midori, S Tamiyoshi, Kinuyo, et al. Effects of pH and metal ions on antioxidative activities of catechins[J]. Biosci Biotechnol Biochem, 2000(65): 126~132.
[19]	Ogumlewa J O, Osegbe D N.Zinc and cadmium concentrations in indigenous blacks with normal, hypertrophic and malignante prostate[J]. Cancer, 1989(63): 1388~1392.
[20]	Satoh M, Koyama H, Kaji T, et al. Perspectives on cadmium toxicity research[J]. Tohoku J Exp Med 2002, 196(1): 23~32.
[21]	Reed M J.The uptake of testosterone and Zinc in vitro by the human hypertrophic prostate[J]. J Endo, 1973(58): 405.

Effect of Interactions Between EGCG and Zn²⁺ on the Growth of Prostate Cancer PC-3 Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 21

Related Articles 15

Metrics

Comments

Recommended 10

[1]	LIU Yajun, WANG Peiqiang, JIANG Xiaolan, ZHUANG Juhua, GAO Liping, XIA Tao. Research Progress on the Biosynthesis of Monomeric and Polymeric Catechins in Camellia sinensis [J]. Journal of Tea Science, 2022, 42(1): 1-17.
[2]	MA Bingsong, WANG Jiacai, XU Chengcheng, REN Xiaoying, MA Cunqiang, ZHOU Binxing. Differences of Phenolic Components in Puer Raw Tea with Various Storage Periods and Their Effects on the in vitro Antioxidant Capacities [J]. Journal of Tea Science, 2022, 42(1): 51-62.
[3]	FANG Hongfeng, ZHANG Huixia, WANG Guohong, YANG Minhe. Fungal Mixed Fermentation for The Production of Lipase and Its Activity Analysis in Galloylated Catechin Hydrolysis [J]. Journal of Tea Science, 2019, 39(1): 88-97.
[4]	RAN Wei, ZHANG Jin, ZHANG Xin, LIN Songbo, SUN Xiaoling. Infestation of Ectropis obliqua Affects the Catechin Metabolism in Tea Plants [J]. Journal of Tea Science, 2018, 38(2): 133-139.
[5]	ZHANG Yue, HU Yunfei, WANG Shumao, KE Zixing, LIN Jinke. Bioinformatic Analysis of MYB Transcription Factors Involved in Catechins Biosynthesis in Tea Plant (Camellia sinensis) [J]. Journal of Tea Science, 2018, 38(2): 162-173.
[6]	QIAO Ruying, LI Ming, ZHENG Xinqiang, LU Jianliang, YE Jianhui, WANG Kairong, LIANG Yuerong. Inhibitory Effects of Tea and Tea Catechins on Breast Cancer [J]. Journal of Tea Science, 2016, 36(6): 557-566.
[7]	JIN Hongna, SONG Yewei, CUI Weibo, XIE Hong. Effects of Catechins on Acute Hyperuricemia in Mice [J]. Journal of Tea Science, 2016, 36(4): 347-353.
[8]	XU Bin, XUE Jinjin, JIANG Heyuan, ZHANG Jianyong, WANG Yan. Review on Theasinensins in Tea [J]. Journal of Tea Science, 2014, 34(4): 315-323.
[9]	ZHANG Cong, TAN Rong, KONG Junhao, GE Qingfeng, YANG Xiufang, WANG Zhijun. Research on the Antihyperlipdemic Activity of Theaflavins, Catechins and Saponius Mixture Preparations [J]. Journal of Tea Science, 2014, 34(4): 401-407.
[10]	WANG Xue-min, MA Jian-qiang, JIN Ji-qiang, MA Chun-lei, YAO Ming-zhe, CHEN Liang. Genetic Variation of Catechins and Alkaloids in First Generation of Tea Plants [J]. Journal of Tea Science, 2013, 33(5): 397-404.
[11]	LONG Dan, JIANG He-yuan, ZHANG Jian-yong, BAI Yan, WANG Wei-wei. Progress on Molecular Modification for Catechins and Their Application [J]. Journal of Tea Science, 2013, 33(1): 1-12.
[12]	CHEN Lin, CHEN Jian, ZHANG Ying-gen, WU Ling-sheng, WANG Zhen-kang, YANG Wei, YOU Zhi-ming. Variation of Catechins and Purine Alkaloids Fingerprints during the Formation of Fresh Scent-Flavor Oolong Tea [J]. Journal of Tea Science, 2011, 31(6): 493-503.
[13]	NIE Zhi-yin, LIU Ya-jun, LIU Li, GAO Li-ping, XIA Tao. Identification and Reaction Assay of Galloylated Catechins Hydrolase in Tea Plant [J]. Journal of Tea Science, 2011, 31(5): 439-446.
[14]	YANG Li-cong, ZHENG Guo-dong, WANG Chun-rong, LI Dong-ming. Combined Effects of Caffeine and Catechins on the Body Weight and Lipid Metabolism in Mice [J]. Journal of Tea Science, 2010, 30(5): 374-378.
[15]	CAI Shu-xian, LIU Zhong-hua, HUANG Jian-an, LUO Guo-an, YU Xing-long, DONG Xin-rong, YANG Zhi-hui, YE Fei. Study on Inhibiting Effects of Tea Catechins on the Formation of Age Pigment Fluorescence Products [J]. Journal of Tea Science, 2010, 30(1): 1-8.

Effect of Interactions Between EGCG and Zn2+ on the Growth of Prostate Cancer PC-3 Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 21

Related Articles 15

Metrics

Comments

Recommended 10

Effect of Interactions Between EGCG and Zn²⁺ on the Growth of Prostate Cancer PC-3 Cells