配体交换色谱手性流动相法拆分和定量茶氨酸对映体

doi:10.13305/j.cnki.jts.2006.04.009

Abstract

Abstract: Ligand exchange chromatography with chiral mobile phases was established for the separation of theanine enantiomers.The optimum condition was :Polaris C₁₈column, mobile phase: L-proline: Cu²⁺=2:1, the concentration of Cu²⁺was 0.5βmmol/L, pH6.8, the volume of methanol is 2%, wavelength: 254βnm;flow rate:0.9βml/min, column temperature:30℃. There was a good linearity between peak areas and injection quantities of L-Theanine ranging from 0.09542βμg~4.241βμg and D-Theanine ranging from 0.08486βμg~4.243βμg. The recovery of L-Theanine range from 97.45%~100.4% and D-Theanine range from 97.07%~100.1%.

Key words: HPLC, theanin enantiomer, separation, chiral mobile phases

CLC Number:

S571.1
O657.7

LI Yin-hua, LIU Zhong-hua, HUANG Jian-an. Separation and Quantitation of Theanine Enantiomers on Ligand Exchange Chromatography with Chiral Mobile Phase[J]. Journal of Tea Science, 2006, 26(4): 280-284.

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Separation and Quantitation of Theanine Enantiomers on Ligand Exchange Chromatography with Chiral Mobile Phase

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