酶性氧化合成茶黄素条件优化的研究

doi:10.13305/j.cnki.jts.2006.04.010

Abstract

Abstract: Obtain the optimum reaction condition of synthesizing theaflavins by enzymatic oxidation, synthesizing theaflavins was studied by raw ppo extracted from fresh tea leaves under single factor and orthogonal experimental design. Based on these results, synthesizing theaflavins was also studied by using breaking fresh tea leaves. The result showed that the optimum condition of theaflavins synthesizing is 30℃ temperature is, under this reaction system, pH value of the present system is 4.8, the concentration of substrates is 0.9βg/100βml, the concentration of enzymes is 28βg/100βml (based on the fresh weight), oxygen rate is 0.4βL/min. Temperatures and pH values in the reaction system are the most important factors(P<0.05), and adding suitable oxygen is another necessary factor in theaflavins synthesis by enzymatic oxidation.

Key words: theaflavins, catechin, enzymatic oxidation, optimization

CLC Number:

S571.1

GU Ji-ping, LIU Zhong-hua, HUANG Jian-an, SHI Zhao-peng. Study on the Optimum Condition of Synthesizing Theaflavins by Using Enzyme-catalysing Oxidation[J]. Journal of Tea Science, 2006, 26(4): 285-290.

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Study on the Optimum Condition of Synthesizing Theaflavins by Using Enzyme-catalysing Oxidation

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