流动注射化学发光法测定茶叶对羟自由基的清除率

doi:10.13305/j.cnki.jts.2010.03.002

Abstract

Abstract: A new chemiluminescence (CL) system was established by mixing methylene blue solution with Fe²⁺-H₂O₂ solution in acidic medium for the determination of scavenging effect of tea on hydroxyl radical. The CL intensity decreased when the extractives of tea were added to the reaction system, indicating that the hydroxyl free radicals（·OH）were scavenged partially in the solution. Based on this principle, a new method was developed to determine the scavenging effect of tea on hydroxyl radical, and the antioxidantive activities of 8 kinds of teas were determined. The results showed that green tea demonstrated the strongest antioxidantive activity.

Key words: flow injection, chemiluminescence, methylene blue, tea, hydroxyl radical(·OH), antioxidant activity

CLC Number:

TS272
O657.3

CAI Zhuo, JIANG Cai-ying, ZHAO Jing, MO Li-jun, ZHANG Xian, CHEN Qing-quan. Determination of Scavenging Effect of Tea on Hydroxyl Radical by Flow Injection Chemiluminescence Method[J]. Journal of Tea Science, 2010, 30(3): 163-166.

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Determination of Scavenging Effect of Tea on Hydroxyl Radical by Flow Injection Chemiluminescence Method

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