绿茶几种化学组分苦涩味非线性回归分析及在感官审评中的应用

doi:10.13305/j.cnki.jts.2010.05.011

Abstract

Abstract: The methods of quantitative response scales and sequential analysis for organoleptic tests were employed to quantitatively evaluate the bitterness and astringency of tea infusions. Flavor characteristic curves (the relationship between taste intensity and concentration) of EGCG and caffeine were established. The mathematical functions of bitterness and astringency were built based on three main compounds namely EGCG (epigallocatechin-3-O- gallate), caffeine and rutin, and the interactions of the three compounds on bitterness and astringency were examined. The results showed that rutin could increase the bitterness of caffeine, but it had no effect on flavor of EGCG, while caffeine could enhance astringency of EGCG. The functions were used to discover the mysteries of heavy bitterness and astringency of green tea produced in Sichuan province. The results from the quantification of main tastants and flavor of twenty tea samples showed that the functions could give the explanation of 78 to 137 percents to the bitterness and 68 to 144 percents to the astringency. The heavy bitterness and astringency of Sichuan green tea were mainly caused by high contents of EGCG and caffeine.

Key words: Sichuan green tea, bitterness and astringency, EGCG, caffeine, nonlinear regression analysis

CLC Number:

XU Wen-ping, LI Da-xiang, ZHANG Zheng-zhu, TANG Qian, WAN Xiao-chun. The Nonlinear Regression of Bitterness and Astringency of Main Compounds in Green Tea and the Application in Organoleptic Tests[J]. Journal of Tea Science, 2010, 30(5): 399-406.

References

[1] 陆松侯, 施兆鹏. 茶叶审评与检验[M]. 北京: 中国农业出版社, 2001.
[2] 李燕, 刘清君, 徐莹. 味觉传导机理及味觉芯片技术研究进展[J]. 科学通报, 2005(14): 1425-1433.
[3] Lawless H T,Corrigan C J,Lee C B.Interactions of astringent substances[J]. Chemical Senses, 1994, 19(2): 141-154.
[4] 张水华, 徐树来, 王永华. 食品感官分析与实验[M]. 北京: 化学工业出版社, 2006.
[5] Di Natale C, Macagnano A, Davide F.Multicomponent analysis on polluted waters by means of an electronic tongue[J]. Sensors and Actuators B-Chemical, 1997, 44(1/2/3): 423-428.
[6] Winquist F, Wide P, Lundstrom I.The combination of an electronic tongue and an electronic nose for improved classification of fruit juices[J]. Eurosensors Xii, 1998(1/2): 1087-1090, 1205.
[7] Chen Q S,Zhao J W,Vittayapadung S.Identification of the green tea grade level using electronic tongue and pattern recognition[J]. Food Research International, 2008, 41(5): 500-504.
[8] Zoltan K,Balazs K D,Andras F.Qualitative analysis of fruit juice by electronic tongue[J]. Elelmiszervizsgalati Kozlemenyek, 2008, 54(3): 151-162.
[9] 王俊, 胡桂仙, 于勇. 电子鼻与电子舌在食品检测中的应用研究进展[J]. 农业工程学报, 2004(2): 292-295.
[10] 施兆鹏, 陈国本, 曾秋霞. 夏茶苦涩味的形成与内质成分的关系[J]. 茶叶科学, 1984, 4(1): 61-62.
[11] 施兆鹏, 刘仲华. 夏茶苦涩味化学实质的数学模型探讨[J]. 茶叶科学, 1987, 7(2): 7-12.
[12] Scharbert S,Holzmann N,Hofmann T.Identification of the astringent taste compounds in black tea infusions by combining instrumental analysis and human bioresponse[J]. Journal of Agricultural and Food Chemistry, 2004, 52(11): 3498-3508.
[13] Scharbert S, Hofmann T.Molecular definition of black tea taste by means of quantitative studies, taste reconstitution, and omission experiments[J]. Journal of Agricultural and Food Chemistry, 2005, 53(13): 5377-5384.
[14] 曾广植, 魏诗泰. 味觉的分子识别[M]. 北京: 科学出版社, 1984.
[15] 程启坤, 阮宇成, 王月根. 绿茶滋味化学鉴定法[J]. 茶叶科学, 1985, 5(1): 7-17.
[16] ISO 8586-1. British Standards Institution. Assessors for sensory analysis —part 1: Guide to the selection, training and monitoring of selected assessorsSO 8586-1. British Standards Institution. Assessors for sensory analysis —part 1: Guide to the selection, training and monitoring of selected assessors[S]. London: British Standards Institution, 1993.
[17] ISO 4121. British Standards Institution. Sensory analysis —guidelines for the use of quantitative response scalesSO 4121. British Standards Institution. Sensory analysis —guidelines for the use of quantitative response scales[S]. London: British Standards Institution, 2003.
[18] ISO 16820. British Standards Institution. Sensory analysis—methodology—sequential analysisSO 16820. British Standards Institution. Sensory analysis—methodology—sequential analysis[S]. London: British Standards Institution, 2004.
[19] 李大祥, 陈跃华, 魏志文. 茶叶中主要多酚类物质和咖啡碱的HPLC测定方法[C]//李增智,姚佐文. 现代农业理论与实践—安徽现代农业博士科技论坛论文集, 安徽: 安徽大学出版社, 2007: 40-42.
[20] 崔桂友. 谷氨酸一钠和5′-核苷酸的呈味性质[J]. 中国烹饪研究, 1996(2): 17-20.

The Nonlinear Regression of Bitterness and Astringency of Main Compounds in Green Tea and the Application in Organoleptic Tests

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