茶黄素双没食子酸酯的抗癌活性及其作用机理研究

doi:10.13305/j.cnki.jts.2007.01.005

Abstract

Abstract: Theaflavin digallate was purified by Sephadex LH-20 column chromatography. Its anticancer activity and mechanism were analyzed on H1299 and HCT-116 cancer cell lines. Results from MTT assay showed that Theaflavin digallate could inhibit the growth of H1299, and its IC₅₀ value is 25μmol/L. Theaflavin digallate could slightly adjust the cell cycle of HCT-116 cancer cell, and significantly promote the apoptosis of HCT-116 cancer cell at 50μmol/L. Theaflavin digallagte could decrease the expression of cancer-promoting biomarker, Bcl-xL, and increase the expression of cancer-inhibiting biomarker, Bax, on HCT-116 cancer cell line.

Key words: theaflavin, anticancer, cell cycle, apoptosis, western blotting

CLC Number:

S571.1
S318

JIANG He-yuan, Hang Xiao, YUAN Xin-yue, WANG Chuan-pi, Chung S Yang. Anticancer Activity of Theaflavin Diagallate and its Mechanism[J]. Journal of Tea Science, 2007, 27(1): 33-38.

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Anticancer Activity of Theaflavin Diagallate and its Mechanism

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