茶树ACC合成酶基因全长cDNA的克隆及其生物信息学分析

doi:10.13305/j.cnki.jts.2008.04.013

Abstract

Abstract: Ethylene is one of the important plant hormones. It has significant physiological role in the life of plant. ACC (1-aminocyclopropane-1-carboxylic acid) synthase (ACS) is a key enzyme in the ethylene biosynthesis in plants. It plays important roles in the regulating ethylene biosynthesis. Degenerate primers were designed based on the conservative ACS gene sequence of other plants in the GenBank to clone the full-length cDNA of ACS genes of the tea plant by using the RACE and RT-PCR methods. It is deposited to the GenBank (Accession No. EF205149). The tea plant ACS gene cDNA is 1 579 bp in length, encoding 478 amino acids, the putative molecular weight is 53.7 kD and the pI is 8.039. The phylogenetic trees constructed by the software MEGA 3 showed that ACS gene of tea plant was closely related to that of Diospyros kaki. The cloning of ACS gene full-length cDNA from the tea plant provides a possibility for understanding the molecular mechanism of physiological role on molecular level in the tea plant.

Key words: tea plant, ethylene, ACC synthase, cloning, bioinformatics

CLC Number:

S571.1
Q523

ZHANG Ya-li, QIAO Xiao-yan, CHEN Liang. Full-length cDNA Cloning and Bioinformatic Analysis of ACC Synthase Gene from the Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2008, 28(4): 235-241.

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Full-length cDNA Cloning and Bioinformatic Analysis of ACC Synthase Gene from the Tea Plant (Camellia sinensis)

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