安吉白茶正常与白化叶片基因表达差异的初步研究

doi:10.13305/j.cnki.jts.2008.01.010

Abstract

Abstract: Differences of gene expression between normal and albino leaves of tea plant (Camellia sinensis cv. Baiye1) were studied by mRNA differential display reverse transcription-PCR (DDRT-PCR). Fifty-eight differentially expressed cDNA fragments were obtained from normal and albino leaves. Twelve of which were confirmed by the analysis of RT-PCR. Among them, 5 fragments were specially expressed in normal leaves, 4 fragments were specially expressed in albino leaves, 1 fragment was up-regulated in normal leaves, and 2 fragments were up-regulated in albino leaves. Through BLASTX analysis with GenBank, 5 cDNA fragments showed high homologous to five known genes, namely soul heme-binding family protein of Arabidopsis thaliana, methionine synthase of Beta vulgaris, gag-pol polyprotein-related in Medicago truncatula, human glyceraldehyde-3-phosphate dehydrogenase pseudogene and ACC synthase of rose, respectively. The other 7 might be novel genes, they showed poor homologous to all proteins in the database.

Key words: Camellia sinensis cv. Baiye1, Albino, Gene expression difference, mRNA differential display reverse transcription-PCR, RT-PCR

CLC Number:

S571.1
Q523

WANG Xin-chao, ZHAO Li-ping, YAO Ming-zhe, CHEN Liang, YANG Ya-jun. Preliminary Study on Gene Expression Differences between Normal Leaves and Albino Leaves of Anji Baicha (Camellia sinensis cv. Baiye1)[J]. Journal of Tea Science, 2008, 28(1): 50-55.

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Preliminary Study on Gene Expression Differences between Normal Leaves and Albino Leaves of Anji Baicha (Camellia sinensis cv. Baiye1)

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