乌龙茶种质资源种群遗传多样性AFLP评价

doi:10.13305/j.cnki.jts.2004.03.007

Abstract

Abstract: Genetic diversity in 45 Oolong germplasms collected from three provinces, Fujian, Guangdong and Taiwan was evaluated by AFLP fingerprinting based on silver staining technique using 5 primer combinations. The 5 primer combinations generated 208 polymorphic bands. Largest genetic distance between germplasm was 0.481, and the smallest was 0.124. Estimated genetic diversity within the 45 germplasm was up to 0.311. Genetic diversity within Wuyi Mountain population was highest, while genetic diversity within Anxi population was higher than Guangdong and Taiwan populations and lowest within Taiwan population. A dendrogram generated by NT-SYS software indicated that the 45 Oolong tea germplasms could be divided as Wuyi type and Chaoan type. Genetic similarity determined by Popgene 32 software was highest between Anxi and Wuyi populations which was up to 0.9505, and lowest between Taiwan and Chaoan populations which was up to 0.77. It could be inferred that Fujian Oolong tea germplasms originated from Wuyi Mountains.

Key words: Tea Plant, Camellia sinensis, Oolong tea germplasm, Genetic diversity, AFLP

CLC Number:

S571.1
Q523

HUANG Fu-ping, LIANG Yue-rong, LU Jian-liang, CHEN Rong-bing, Mamati G. E., SUN Qing-lei. Evaluation of Genetic Diversity in Oolong Tea Germplasms by AFLP Fingerprinting[J]. Journal of Tea Science, 2004, 24(3): 183-189.

References 15

[1]	吴敏生, 戴景瑞. 扩增片段长度多态性(AFLP): 一种新的分子标记技术[J]. 植物学通报, 1998, 15(4):68-74.
[2]	Paul S, Wachira F.N, Powell W, et al. Diversity and genetic differentiation among populations of India and Kenyan tea (Camellia Sinensis (L.) O. Kuntze) revealed by AFLP markers[J]. Theor Appl.Genet, 1997, 94: 255-263.
[3]	Wachira F.N., Waugh R., Hackett C.A, et al. Detection of genetic diversity in tea [camellia sinensis] using RAPD makers[J]. Genome, 1995, Vol.38: 201-210.
[4]	Hackett C.A, Wachira F.N, Paul S, et al. Construction of a genetic linkage map for Camellia sinensis(tea)[J]. Heredity, 2000, 346-355.
[5]	Wachira F, Tanaka J, Takeda Y. Genetic variation and differentiation in tea (camellia sinensis) germplasm revealed by RAPD and AFLP variation[J]. Journal of Horticultural Science & Biotechnology, 2001, 76(5): 557-563.
[6]	Balasaravanan B, Pius P. K, Kumar R. R., et al. Genetic diversity among south Indian tea germplasm(camellia sinensis,C.assamica and C. assamica spp.lasiocalyx)using AFLP markers[J]. Plant Science, 2003, 165: 365-372.
[7]	Clark M.S.主编（顾红雅，瞿礼嘉译）.植物分子生物学－实验手册。北京：高等教育出版社，1999, 6-7.
[8]	Vos P, Hogers R., Bleeker M., et al. AFLP: a new technique for fingerprinting[J].Nucleic Acids research, 1995, Vol.23, No.21: 4407-4414.
[9]	Bassam B.J, Anolles G C, Gresshoff P M. Fast and sensitive silver staining of DNA in Polyacrylamide Gels[J]. Analyticl Biochemistry, 1991, 196, 80-83.
[10]	Nei M. and Li, W.H..Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proc. Natl. Acad. Sci. USA, 1979, 76: 5269-5273.
[11]	Nei M. Analysis of gene diversity in subdivided populations[J]. Proc.Natl.Acad.Sci.USA, 1973, 76: 5269-5273.
[12]	Shannon C.E, Weaver W. The mathemmatical theory of communication[M].University of Illinois Press, 1949, Urbana, USA.
[13]	Yeh F.C, Yang R.C, Boyle T.B.J, et al. POPGENE,the user friendly shareware for population genetic analysis.Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
[14]	倪郑重, 何融. 乌龙茶工艺史考证[J]. 中国科技史料, 1995, 16(3): 92-96.
[15]	阮逸明. 台湾乌龙茶的发展及特色[J]. 福建茶叶, 2003, 1: 21-23.

Evaluation of Genetic Diversity in Oolong Tea Germplasms by AFLP Fingerprinting

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References 15

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City [J]. Journal of Tea Science, 2022, 42(5): 601-609.
[2]	ZHOU Hanchen, YANG Jihong, XU Yujie, WU Qiong, LEI Pandeng. Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis [J]. Journal of Tea Science, 2022, 42(5): 638-648.
[3]	CHEN Qiyu, MA Jianqiang, CHEN Jiedan, CHEN Liang. Genetic Diversity of Mature Leaves of Tea Germplasms Based on Image Features [J]. Journal of Tea Science, 2022, 42(5): 649-660.
[4]	SUN Yue, WU Jun, WEI Chaoling, LIU Mengyue, GAO Chenxi, ZHANG Lingzhi, CAO Shixian, YU Shuntian, JIN Shan, SUN Weijiang. Screening of Tea Germplasm Resistant to Matsumurasca onukii and Dendrothrips minowai Priesner and Analysis of Resistance-related Factors [J]. Journal of Tea Science, 2022, 42(5): 689-704.
[5]	WANG Yuyuan, LIU Renjian, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Expression Analysis and Functional Identification of CsTT2 R2R3-MYB Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2022, 42(4): 463-476.
[6]	ZHAO Dongwei. Nomenclature, Typification, and Natural Distribution of Camellia sinensis var. assamica (Theaceae) [J]. Journal of Tea Science, 2022, 42(4): 491-499.
[7]	XING Anqi, WU Zichen, XU Xiaohan, SUN Yi, WANG Genmei, WANG Yuhua. Research Advances of Fluoride Accumulation Mechanisms in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 301-315.
[8]	WANG Tao, WANG Yiqing, QI Siyu, ZHOU Zhe, CHEN Zhidan, SUN Weijiang. Identification and Transcriptional Regulation of CLH Gene Family and Expression Analysis in Albino Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 331-346.
[9]	LIU Renjian, WANG Yuyuan, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants [J]. Journal of Tea Science, 2022, 42(3): 347-357.
[10]	LIU Fuhao, FAN Yangen, WANG Yu, MENG Fanyue, ZHANG Lixia. Screening and Identification of Chaperone CsHIPP26.1 Chelating Ions in Tea Cultivar ‘Huangjinya’ [J]. Journal of Tea Science, 2022, 42(2): 179-186.
[11]	YANG Ni, LI Yimin, Li Jingwen, TENG Ruimin, CHEN Yi, WANG Yahui, ZHUANG Jing. Effects of Exogenous 5-ALA on the Chlorophyll Synthesis and Fluorescence Characteristics and Gene Expression of Key Enzymes in Tea Plants under Drought Stress [J]. Journal of Tea Science, 2022, 42(2): 187-199.
[12]	CHEN Xiaomin, ZHAO Feng, WANG Shuyan, SHAO Shuxian, WU Wenxi, LIN Qin, WANG Pengjie, YE Naixing. Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea [J]. Journal of Tea Science, 2022, 42(1): 18-28.
[13]	WANG Pengjie, YANG Jiangfan, ZHANG Xingtan, YE Naixing. Research Advance of Tea Plant Genome and Sequencing Technologies [J]. Journal of Tea Science, 2021, 41(6): 743-752.
[14]	ZHOU Hanchen, LEI Pandeng. The Functional Identification of Two Alternative Splicing Transcripts of CsNES [J]. Journal of Tea Science, 2021, 41(6): 753-760.
[15]	TANG Lei, XIAO Luodan, HUANG Yifan, XIAO Bin, GONG Chunmei. Cloning and Magnesium Transport Function Analysis of CsMGTs Genes in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2021, 41(6): 761-776.