茶树AFLP分子连锁图谱的构建

doi:10.13305/j.cnki.jts.2005.01.002

Abstract

Abstract: Based on an improved method of AFLP, AFLP markers were employed for construction of a linkage map used a set of 69 F1 progenies of Qi Men No.4×Chao An Da Wu Ye as a mapping population. In this work, all together 1925 bands were obtained by 22 pairs of primers and 87.5 bands each primer pair on average, a total of 485 (25.19%) polymorphic AFLP markers were detected. The 356 (73.40%) of polymorphic markers with Mendelian segregation ratio (P=0.01) were obtained. 247（69.38%）of the 356 loci were 1:1 segregation, 109（30.62%）of the 356 loci were 3:1 segregation. Furthermore, the AFLP molecular markers linkage maps of Qi Men No.4 and Chao An Da Wu Ye were constructed respectively by using Mapmaker/Exp (version3.0). The map of female parent included 17 linkage groups and located 208 markers, covered total map length of 2457.7 cM, and average distance between markers was 11.9 cM. The map of male parent included 16 linkage groups and located 200 markers,covered total map length of 2545.3 cM, and average distance between markers was 12.8 cM.

Key words: Tea plant, Molecular markers linkage map, AFLP

CLC Number:

S571.1
Q523

HUANG Jian-an, LI Jia-xian, HUANG Yi-huan, LUO Jun-wu,GONG Zhi-hua, LIU Zhong-hua. Construction of AFLP Molecular Markers Linkage Map in Tea Plant[J]. Journal of Tea Science, 2005, 25(1): 7-15.

References 31

[1]	Zabeau M, Vos P. Selective restriction fragment amplification:a general method for DNA fingerprinting. European Patent Application, 1993, 92402629, 7 (publication No. 0534858 A1).
[2]	田中淳一. RAPDをべースにしたチャの连锁地图の作成と遗传解析への利用の可能性[J]. 茶业研究报告, 1996,84(别册):44~45.
[3]	Hackett C A, Wachira F N, Paul S, et al. Construction of a genetic linkage map for Camellia sinensis(tea). Heredity, 2000,85(4):346~355.
[4]	Hemmat M, Weeden N F, Manganaris A G, et al. Molecular marker linkage for apple [J]. The Journal of Heredity, 1994:85(1):4~11.
[5]	Grattapaglia D, Sederoff R. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: Mapping strategy and RAPD markers[J], Genetics, 1994, 137:1121~1137.
[6]	黄建安, 黄意欢, 罗军武, 等. 茶树基因组DNA的高效提取方法[J]. 湖南农业大学学报, 2003, 29(5): 402~407.
[7]	Vos P, Hogers R, Bleeker M, et al. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 1995, 23: 4407~4414.
[8]	GIBCOBRL. Instruction manual AFLPTM analysis system, AFLP start primer kit. http://plantbio.berkeley.edu.
[9]	SSR gel and silver staining protocol. http://dendrome. ucdavis.edu.
[10]	Silver sequenceTM DNA sequencing system. http://www.promega.com.
[11]	尹佟明, 黄敏仁, 王明麻. 利用RAPD标记构建响叶杨和银白杨分子标记连锁图谱[J]. 植物学报, 1999, 41(9): 956~961.
[12]	张新叶, 尹佟明, 诸葛强, 等. 利用RAPD标记构建美洲黑杨×欧美杨分子标记连锁图谱[J]. 遗传, 2000, 22(4): 209~213.
[13]	Crattapaglia D, Sederoff R. Cenetic mapping of QTLs controlling vegetative propagation in Euclypttas grandis and E.urophylla using a pseudotestcross mapping strategy and RAPID markets [J]. Theor Appl Genet, 1995,90:933~947.
[14]	Maliepaard C, Alston F H. Aligning male and female linkage maps of apple using multi-alletic markers [J]. Theor Appl Genet, 1998, 97: 60~73.
[15]	尹佟明, 黄敏仁, 朱立煌. 利用显性分子标记和F1群体进行林木遗传连锁图谱的构建[J]. 生物工程进展, 1996, 16(4): 12~16.
[16]	张德强. 毛白杨遗传连锁图谱的构建及重要性状的分子标记[D]. 北京林业大学博士学位论文, 2002.
[17]	赵爱春, 鲁成, 李斌, 等. 家蚕AFLP分子连锁图谱的构建及绿茧基因定位[J]. 遗传学报, 2004, 31(8): 787~794.
[18]	Testolin R, Huang W.G, Lain O, et al. A kiwifruit (Actinidia spp.) linkage map based on microsatellites and integrated with AFLP markers [J]. Theor Appl Genet, 2001 ,103: 30~36.
[19]	Porceddu A, Albertini E, Barcaccia G, et al. Linkage mapping in apomictic and sexual Kentucky bluegrass (Poa pratensis L.) genotypes using pseudo-testcross strategy based on AFLP and SAMPL markers [J]. Theor Appl Genet, 2002, 104: 273~280.
[20]	岳志芹, 王伟继, 孔杰, 等. AFLP分子标记构建中国对虾遗传连锁图谱的初步研究[J]. 高技术通讯, 2004(5): 88~93.
[21]	Debener T, Mattiesch L. Construction of a genetic linkage map for roses using RAPD and AFLP markers [J]. Theor Appl Genet, 1999, 99: 891~899.
[22]	Margues C M, Araujo J A, Ferreira J G, et al. AFLP genetic maps of Eucalyptus globules and E. tereticomis [J]. Theor Appl Cenet. 1998, 96(6): 727~737.
[23]	Qi X, Stam P, Lindhout P, et al. Use of locus-specific AFLP markers to construct a high-density molecular map in barley [J]. Theor Appl Genet, 1998, 96: 376~384.
[24]	陈书霞, 王晓武, 方智远, 等. RAPD标记构建芥蓝×甘蓝分子标记图谱[J]. 园艺学报, 2002, 29(3): 229~232.
[25]	Haanatra J P W, Wye C, Verbakel H, et al. An integrated high density RFLP-AFLP map of tomato based on two Lycopersicon esculentum×L.pennellii F2 populations [J]. Theor Appl Genet, 1999, 99: 254~271.
[26]	吴晓雷, 贺超英, 王永军, 等. 大豆遗传图谱的构建和分析[J]. 遗传学报, 2001, 28(11): 1051~1061.
[27]	于拴仓, 王永健, 郑晓鹰. 大白菜分子遗传图谱的构建与分析[J]. 中国农业科学, 2003, 36(2): 190~195.
[28]	王美, 张凤兰, 孟祥栋, 等. 中国白菜AFLP分子遗传图谱的构建[J]. 华北农学报, 2004, 19(1): 28~33.
[29]	熊立仲, 王石平, 刘克德, 等. 微卫星DNA和AFLP标记在水稻分子标记连锁图上的分布[J]. 植物学报, 1998, 40(7): 605~614.
[30]	郭得平, 卢钢. 利用AFLP分子标记构建杂种葱的遗传图谱[J]. 中国农业科学, 2004, 37(4): 584~587.
[31]	Maheswaran M, Subudhi P K, Nandi S, et al. Polymorphism, distribution and segregation of AFLP markers in a doubled-haploid rice population [J]. Theor Appl Genet, 1997, 94: 39~45.

Construction of AFLP Molecular Markers Linkage Map in Tea Plant

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References 31

Related Articles 15

Metrics

Comments

Recommended 10

[1]	CHEN Qiyu, MA Jianqiang, CHEN Jiedan, CHEN Liang. Genetic Diversity of Mature Leaves of Tea Germplasms Based on Image Features [J]. Journal of Tea Science, 2022, 42(5): 649-660.
[2]	SUN Yue, WU Jun, WEI Chaoling, LIU Mengyue, GAO Chenxi, ZHANG Lingzhi, CAO Shixian, YU Shuntian, JIN Shan, SUN Weijiang. Screening of Tea Germplasm Resistant to Matsumurasca onukii and Dendrothrips minowai Priesner and Analysis of Resistance-related Factors [J]. Journal of Tea Science, 2022, 42(5): 689-704.
[3]	WANG Yuyuan, LIU Renjian, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Expression Analysis and Functional Identification of CsTT2 R2R3-MYB Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2022, 42(4): 463-476.
[4]	LIU Renjian, WANG Yuyuan, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants [J]. Journal of Tea Science, 2022, 42(3): 347-357.
[5]	YANG Ni, LI Yimin, Li Jingwen, TENG Ruimin, CHEN Yi, WANG Yahui, ZHUANG Jing. Effects of Exogenous 5-ALA on the Chlorophyll Synthesis and Fluorescence Characteristics and Gene Expression of Key Enzymes in Tea Plants under Drought Stress [J]. Journal of Tea Science, 2022, 42(2): 187-199.
[6]	CHEN Xiaomin, ZHAO Feng, WANG Shuyan, SHAO Shuxian, WU Wenxi, LIN Qin, WANG Pengjie, YE Naixing. Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea [J]. Journal of Tea Science, 2022, 42(1): 18-28.
[7]	TANG Lei, XIAO Luodan, HUANG Yifan, XIAO Bin, GONG Chunmei. Cloning and Magnesium Transport Function Analysis of CsMGTs Genes in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2021, 41(6): 761-776.
[8]	WU Wanfu, LYU Shidong, YANG Xuefang, LI Yue, ZHANG Jiguang. Study of Characteristic Indexes and Fatty Acid Composition in Big-leaf Tea Plant (Camellia sinensis var. assamica) Seed Oils from Yunnan Province [J]. Journal of Tea Science, 2021, 41(4): 463-470.
[9]	LIU Dingding, WANG Junya, TANG Rongjin, CHEN Liang, MA Chunlei. Genome-wide Identification of PPR Gene Family and Expression Analysis of Albino Related Genes in Tea Plants [J]. Journal of Tea Science, 2021, 41(2): 159-172.
[10]	ZHANG Qunfeng, NI Kang, YI Xiaoyun, LIU Meiya, RUAN Jianyun. Advances of Magnesium Nutrition in Tea Plant [J]. Journal of Tea Science, 2021, 41(1): 19-27.
[11]	CHEN Yao, ZHANG Weifu, REN Hengze, XIONG Fei, ZHANG Haojie, YAO Lina, LIU ying, WANG Lu, WANG Xinchao, YANG Yajun, HAO Xinyuan. Genome-wide Investigation and Expression Analysis of DNA Demethylase Genes in Tea Plant (Camellia Sinensis) [J]. Journal of Tea Science, 2021, 41(1): 28-39.
[12]	JIN Ke, HUANG Jian'an, XIONG Ligui, LIU Shuoqian, QIN Xiaohong, PENG Jing, LI Yinhua, LI Juan. Research of Theanine-related Genes Expressed in Etiolated Tea Plant (Camellia Sinensis) [J]. Journal of Tea Science, 2021, 41(1): 40-47.
[13]	WAN Qiwen, YANG Ni, LI Yimin, HAN Miaohua, LIN Shijia, TENG Ruimin, ZHUANG Jing. Effects of Exogenous 24-Epibrassinolide on Photosynthetic Characteristics of Tea Plants [J]. Journal of Tea Science, 2021, 41(1): 58-70.
[14]	HUANG Fangfang, LI Qin, HUANG Jian'an. Research Progress of Tea Rhizosphere Microorganisms [J]. Journal of Tea Science, 2020, 40(6): 715-723.
[15]	FENG Xia, DI Taimei, PENG Jing, LI Nana, YAO Lina, YANG Yajun, WANG Xinchao, WANG Lu. Interaction Identification and Expression Analysis of CsCIPK12 and CsKIN10 in Tea Plant [J]. Journal of Tea Science, 2020, 40(6): 739-750.