EGCG对高糖培养的系膜细胞增殖及周期素激酶抑制剂P27的作用

doi:10.13305/j.cnki.jts.2005.04.012

Abstract

Abstract: The effect of epigallocatechin-3-gallate (EGCG) on rat mesangial cells (GMCs) proliferation cultured in high glucose condition and the expression of cyclin kinase inhibitor p27(p27) protein were investigated. The GMCs were divided into 6 groups. 48 hours later, the proliferation activity of GMCs was observed by MTT assay, the expression of p27 protein was enhanced by Western blot. Results showed that compared with normal glucose group, high glucose treatment significantly increased GMCs proliferation activity and the expression of p27 protein. EGCG in different concentration suppressed the proliferation of GMCs and down-regulated the expression of p27. It can be concluded that EGCG can suppress the proliferation activity of GMCs cultured under high glucose and decrease the expression of p27 protein, which implied EGCG probably decrease the accumulation of extracellular matrix (ECM) and slowed the progession of progression of glomerular hypertrophy and glomerulosclerosis in diabetic nephropathy.

Key words: EGCG, Mesangial cell, Diabetic nephropathy, Cyclin kinase inhibitor p27

CLC Number:

Q946.8

LI Cai-rong, CAI Fei, YANG Xiao, ZHU Zhong-hua. Effect of EGCG on Mesangial Cells Proliferation Cultured Condition and Expression of Cyclin Kinase Inhibitor p27 in High Glucose[J]. Journal of Tea Science, 2005, 25(4): 307-310.

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Effect of EGCG on Mesangial Cells Proliferation Cultured Condition and Expression of Cyclin Kinase Inhibitor p27 in High Glucose

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