儿茶素对铅诱导HepG2细胞氧化损伤及茶多酚对铅毒小鼠体内铅含量的影响

doi:10.13305/j.cnki.jts.2003.02.007

Abstract

Abstract: The effects of tea catechins on lead-induced lipid peroxidation damage in HepG2 cells and tea polyhenol on the content of lead in lead-exposed mice were investigated.HepG2 cell viability was determined using the MTT method,lipid peroxidation was assayed by detetmining the production rate of thibarbiturie acid reactive substance(TBARS)which was expressed as malondialdehyde(MDA)equivalents.The content of lead in lead-exposed mice was determined using hydride atomic fluorescence spectroscopic method. Results showed that tea catechins had protective effects on lead-exposed HepG2 cell viability and inhibitory effects on TBARS formation in HepG2 cells.All the indices of the mice in the exposed groups were significantly higher than those in the control.Tea polyphenol was able to accelerate the removal of lead from liver,but the same effects were not found in blood、kidney, brain and thigbones.

Key words: Tea science, Tea catechins, Tea polyphenol, Lead exposure, Lipid preoxidation, Removal of lead

CLC Number:

R963

PIAO Zai-ri, Wang Yue-fei, YANG Xian-qiang, CHEN Liu-ji, YANG Yong, ZHAO Bao-lu, JIAO Hong-li, ZHOU Dang-yi. Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice[J]. Journal of Tea Science, 2003, 23(2): 119-123.

References

[1] Hermes-Lima M, Pereira B, Bechara E.J.H. Are free radicals involved in lead poisoning?[J]. Xenobiotica 1991.21:1085-1090.
[2] Bechara E.J.H, Medeiros M.H.G, Monteiro H.P, et al. A free radical hypothesis of lead poisoning and inborn porphyrias associated with 5-aminolevulinic acid overload[J]. Quim. Nova. 1993,16:385-392.
[3] Jiun Y.S, Hsien L.T. Lipid peroxidation in workers exposed to lead. Archiv. Environ[J]. Health. 1994,49:256-259.
[4] Monteiro H.P, Abdalla D.S.P, Arcuri A.S, Bechara E.J.H. Oxygen toxicity related to exposure to lead[J]. Clin. Chem. 1985,31:1673-1676.
[5] PC Hsu, MY Liu, CC Hsu, LY Chen,Y L Guo. Effects of vitamin E and:or C on reactive oxygen species-related lead toxicity in the rat sperm[J]. Toxicology. 1998,128:169-179.
[6] Hande Gurer, Hilal Ozgunes, Serdar Oztezcan,Nuran Ercal.Antioxidant role of α-lipoic acid in lead toxicity[J]. Free Radical Biology & Medicine . 1999 ,27:75-81.
[7] Q Guo, BL Zhao, SR Shen, JW Hou, JG Hu, WJ Xin. ESR study on the structure-antioxidant activity relationship of tea catechins and their epimers[J]. Biochemica et Biophysica Acta., 1999.1427:13-23.
[8] Q Guo, BL Zhao, MF Li, SR Shen, WJ Xin. Studies on protective mechanisms of four components of green tea polyphenols against lipid peroxidation in synaptosomes[J]. Biophys Acta, 1996, 1304:210-222.
[9] C A Rice-Evans, NJ Miller, G Paganga.Antioxidant properties of phenolic compounds[J]. Trends Plant Sci. 1997.2:152-159.
[10] Midori kumamoto, Tamiyoshi Sonda, kinuyo Nagayama,masaaki Tabata. Effects of pH and metal ions on antioxidative activities of catechins[J]. Biosci. Biotechnol .Biochem. 2001,65:126-132.
[11] 高贵珍,顾向荣等.茶叶驱铅效果的实验研究[J]. 卫生毒理学杂志, 1995,9(2):110-112.
[12] 高贵珍,顾向荣等.茶叶驱铅效果与应用研究[J]. 卫生毒理学杂志, 1996,10(2):90-92.
[13] Marinovitch M,Lorenzo J,flaminio l.,Ganata A and Galli C.The HepG2 cell line as a possible alternative to isolated hepatocytes in cytotoxicity studies[J]. ATLA 1988,16:16.
[14] Borenfreund E,Babich H and Martinalguacil N.Rapid chemosensitivity assay with human normal and tumor cells in vitro[J]. In Vitro Cell Development and Biology. 1983.26:1030-1034.
[15] 胡秀芳,沈生荣,杨贤强 .茶多酚抗氧化机理研究现状.茶叶科学 ,1999,19(2):93-103.
[16] 郭炳莹,程启坤.茶汤组分与金属离子的络合性能.茶叶科学,1991 ,11(2):139-141.
[17] 王小平,冯爽.茶多酚对铅染毒小鼠体内SOD活性、铅含量的影响[J]. 苏州医学院学报, 2001, 21(5):515-516.

Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	LI Jing, LIN Cairong, HUANG Yan, DENG Xuming, WANG Yiqing, SUN Weijang. Effects of Tea Polyphenols on Agrobacterium-mediated Plant Genetic Transformation System [J]. Journal of Tea Science, 2022, 42(4): 477-490.
[2]	ZHOU Shaofeng, QIAN Yunfei, ZHAO Zhen, CHEN Xuan, LI Xinghui. Effect of the Tea with Different Degrees of Fermentation on the Formation of Tea Scum [J]. Journal of Tea Science, 2022, 42(1): 76-86.
[3]	WU Xin, SONG Feihu, PEI Yongsheng, ZHU Guanyu, JIANG Lebing, NING Wenkai, LI Zhenfeng, LIU Benying. Study on the Tea Quality Changes and Predictions during the Microwave Fixation Process by Machine Vision [J]. Journal of Tea Science, 2021, 41(6): 854-864.
[4]	XIONG Xingping, ZHANG Xinzhong, LI Hongbin, SUN Liang, YAO Mingzhe. Analysis on the Achievements of Tea Science and Technology Awards at Provincial and Ministerial Level and above in China from 2008 to 2019 [J]. Journal of Tea Science, 2021, 41(5): 608-618.
[5]	WANG Shenglin, YANG Chongshan, LIU Zhongyuan, LIU Shanjian, DONG Chunwang. Rapid Detection Method of Tea Polyphenol Content in Black Tea Fermentation Based on Electrical Properties [J]. Journal of Tea Science, 2021, 41(2): 251-260.
[6]	LU Li, CHENG Xi, ZHANG Bo, SHEN Xiaoxia, LIU Yan, XIONG Li, YUAN Xiao, LI Yuanhua, LI Xinghui. Establishment of Predictive Model for Quantitative Analysis of Tea Polyphenols and Caffeine of Souchong by Near Infrared Spectroscopy [J]. Journal of Tea Science, 2020, 40(5): 689-695.
[7]	XIONG Xingping, ZHANG Xinzhong, YANG Zhenwei. Analysis of the Tea Projects Supported by National Natural Science Foundation of China from 1999 to 2019 [J]. Journal of Tea Science, 2020, 40(3): 305-318.
[8]	YAO Min, LI Daxiang, XIE Zhongwen. Recent Advance on Anti-cardiovascular Inflammation of Major Characteristic Compounds in Tea [J]. Journal of Tea Science, 2020, 40(1): 1-14.
[9]	ZHOU Fang, OUYANG Jian, HUANG Jian'an, LIU Zhonghua. Advances in Research on the Regulation of Tea Polyphenols and Effects on Intestinal Flora [J]. Journal of Tea Science, 2019, 39(6): 619-630.
[10]	ZHANG Shuping, WANG Yuefei, XU Ping. Prevention of Tea Polyphenols on Atherosclerosis and Relative Mechanisms [J]. Journal of Tea Science, 2019, 39(3): 231-246.
[11]	ZHU Lin, WU Long, CHEN Xiaoqiang, CHEN Xueling, WU Zhengqi, SHI Yong. Interaction between Tea Polyphenols and Polysaccharides: Progress in Research on Mechanism and Function [J]. Journal of Tea Science, 2019, 39(2): 203-210.
[12]	WU Genliang, HOU Aixiang, LI Ke, LI Zongjun. Effects of Polyphenols of Old Fu Brick Tea on the Elderly Intestinal Flora [J]. Journal of Tea Science, 2018, 38(3): 319-330.
[13]	SHI Chunlin, LI Xiaohuan, HUANG Xiangxiang. Effects of Green Tea Polyphenols on Oxidative Stress Induced by Passive Smoking in Mice Lung [J]. Journal of Tea Science, 2018, 38(2): 212-220.
[14]	SONG Juan, WANG Dong, YU Xudong. Studies on the Preparation and Stability of Tea Polyphenol Emulgel Formulation [J]. Journal of Tea Science, 2017, 37(6): 623-630.
[15]	LEI Liping, ZHU Yuehua, ZHANG Jian, YANG Wenge, LI Puyou, LIU Yanjie, QIAN Yunxia. Effects of Tea Polyphenols on Quality and Microorganisms of Pseudosciaena crocea during Iced Storage [J]. Journal of Tea Science, 2017, 37(5): 523-531.