几种茶多糖降血糖活性的研究

doi:10.13305/j.cnki.jts.2019.06.004

Abstract

Abstract: To study and compare the composition, blood sugar lowering effect and composition of WTP, GTP and BTP. Shoumei, Longjing and Bailingongfu were selected as representatives of white tea, green tea and black tea, and the composition, molecular weight of tea polysaccharides were determined. The mice diabetes model was induced by streptozotocin, metformin was used as a positive control to study the hypoglycemic effect of tea polysaccharides, and qPCR was used to determine the expression level of related genes in mice liver. The results showed that the molecular weights of WTP, GTP and BTP are 18 180 Da, 19 470 Da and 8 745 Da, respectively. The selected tea polysaccharides have hypoglycemic effect, the fasting blood glucose decline rates of WTP, GTP and BTP were 53.2%, 52.8% and 61.6%, respectively. Tea polysaccharides can all improve glucose tolerance, down-regulate the expression of Foxo1, G6Pc, PEPCK and TXNIP genes in mice and there are some differences.

Key words: tea polysaccharides, hypoglycemic, sugar metabolism

CLC Number:

TS201.4

LIU Danqi, REN Fazheng, LI Jingming, HOU Caiyun. Comparative Study on the Structure and Hypoglycemic Activity of Several Tea Polysaccharides[J]. Journal of Tea Science, 2019, 39(6): 652-660.

References 27

[1]	宋林珍, 朱丽云, 高永生, 等. 茶多糖的结构特征与降血糖活性[J]. 食品科学, 2018, 39(19): 162-168.
[2]	Bergenstal R M, Wysham C, Macconell L, et al.Efficacy and safety of exenatide once weekly versus sitagliptin or pioglitazone as an adjunct to metformin for treatment of type 2 diabetes (DURATION-2): a randomised trial[J]. Lancet, 2010, 376(9739): 431-439.
[3]	张阳春, 杨敏, 蔡成永, 等. 粗老茶治糖尿病的药理成分分析[J]. 中草药, 1995(5): 255-257.
[4]	Zhou X, Wang D, Sun P, et al.Effects of soluble tea polysaccharides on hyperglycemia in alloxan-diabetic mice[J]. Journal of Agricultural & Food Chemistry, 2007, 55(14): 5523-5528.
[5]	邵淑宏. 乌龙茶多糖理化性质及抗氧化、降血糖活性研究[D]. 杭州: 浙江大学, 2015.
[6]	Wang Y F, Li Y F, Liu Y Y, et al.Extraction, characterization and antioxidant activities of Se-enriched tea polysaccharides[J]. International Journal of Biological Macromolecules, 2015, 77: 76-84.
[7]	张芸, 倪德江, 陈永波, 等. 乌龙茶多糖调节血脂作用及其机制研究[J]. 茶叶科学, 2011, 31(5): 399-404.
[8]	He N, Shi X, Zhao Y, et al.Inhibitory effects and molecular mechanisms of selenium-containing tea polysaccharides on human breast cancer MCF-7 cells[J]. Journal of Agricultural & Food Chemistry, 2013, 61(3): 579-588.
[9]	Ferreira S S, Passos C P, Madureira P, et al.Structure-function relationships of immunostimulatory polysaccharides: A review[J]. Carbohydr Polym, 2015, 132: 378-396.
[10]	蒋成砚, 谢昆, 薛春丽, 等. 普洱茶多糖抗疲劳作用研究[J]. 安徽农业科学, 2012, 40(1): 154-155.
[11]	倪德江, 谢笔钧, 宋春和. 不同茶类多糖对实验型糖尿病小鼠治疗作用的比较研究[J]. 茶叶科学, 2002, 22(2): 160-163.
[12]	倪德江, 陈玉琼, 谢笔钧, 等. 绿茶、乌龙茶、红茶的茶多糖组成、抗氧化及降血糖作用研究[J]. 营养学报, 2004(1): 57-60.
[13]	孙苏军, 纪海玉, 白云, 等. 坦洋工夫红茶多糖提取工艺优化及其抑制肿瘤活性分析[J]. 食品科学, 2018, 39(4): 254-260.
[14]	张媛媛, 张彬. 苯酚-硫酸法与蒽酮-硫酸法测定绿茶茶多糖的比较研究[J]. 食品科学, 2016, 37(4):158-163.
[15]	中华人民共和国国家质量监督检验检疫总局. 茶叶中茶多酚和儿茶素含量的检测方法: GB/T 8313—2008[S]. 北京: 中国标准出版社, 2008.
[16]	Islam M S, Loots T D.Experimental rodent models of type 2 diabetes: a review[J]. Methods & Findings in Experimental & Clinical Pharmacology, 2009, 31(4): 249. DOI: 10.1358/mf.2009.31.4.1362513.
[17]	黄继汉, 黄晓晖, 陈志扬, 等. 药理试验中动物间和动物与人体间的等效剂量换算[J]. 中国临床药理学与治疗学, 2004, 9(9): 1069-1072.
[18]	玄光善, 潘士佳, 南姬. 桑叶有效成分降糖作用研究[J]. 食品科学, 2011, 32(7): 323-326.
[19]	Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the method[J]. Methods, 2002, 25(4): 402-408.
[20]	闫济民. 糖尿病的典型症状[J]. 中国慢性病预防与控制, 2009, 16(4): 25.
[21]	李秀钧, 钱荣立. 胰岛素抵抗及其临床意义[J]. 中国糖尿病杂志, 1999(3): 163-167.
[22]	Tang W P, Li S M, Liu Y, et al.Anti-diabetic activity of chemically profiled green tea and black tea extracts in a type 2 diabetes mice model via different mechanisms[J]. Journal of Functional Foods, 2013, 5(4): 1784-1793.
[23]	Garancini M P, Calori G, Ruotolo G, et al.Prevalence of NIDDM and impaired glucose tolerance in Italy: an OGTT-based population study[J]. Diabetologia, 1995, 38(3): 306-313.
[24]	黄荷, 夏宁. Foxo1对糖尿病胰腺β细胞的作用[J]. 内科, 2009, 4(3): 404-407.
[25]	Wu J J, Shi S S, Wang H J, et al.Mechanisms underlying the effect of polysaccharides in the treatment of type 2 diabetes: A review[J]. Carbohydrate Polymers, 2016, 144: 474-494.
[26]	Shalev A.Lack of TXNIP protects β-cells against glucotoxicity[J]. Biochemical Society Transactions, 2008, 36(5): 963-965.
[27]	陈小强, 叶阳, 成浩, 等. 不同方法提制的茶叶粗多糖的光谱分析.[J]. 光谱学与光谱分析, 2009, 29(4): 1083-1087.

Comparative Study on the Structure and Hypoglycemic Activity of Several Tea Polysaccharides

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 27

Related Articles 15

Metrics

Comments

Recommended 10

[1]	ZHOU Yang, XIAO Wenjun, LIN Ling, YUAN Dongyin, PENG Yingqi, TAN Chunbo, ZHANG Qiang, GONG Zhihua. Hypoglycemic Effects of Black Tea and Fungus Fermented Black Brick Tea on Hyperglycemic Model Mice [J]. Journal of Tea Science, 2019, 39(4): 415-424.
[2]	YING Le, PAN Yue, WANG Yuefei, XU Ping. Physicochemical Properties, in vitro Antioxidant Activities and Protective Effects of Liubao Tea Polysaccharides on HUVEC [J]. Journal of Tea Science, 2017, 37(1): 25-37.
[3]	WEI Nan, ZHU Qiangqiang, CHEN Jiming, LI Tong, LI Yifan, HUANG Yewei, WANG Xuanjun, SHENG Jun. Tea Polysaccharide Increased Doxorubicin Inhibition of Lung Cancer A549 Cells [J]. Journal of Tea Science, 2016, 36(5): 477-483.
[4]	HUANG Song, LIU Zhonghua, HUANG Jian′an, YANG Heng, LI Qin. Intervention Effects of Fuzhuan Brick Tea Water Extract on Glucose Metabolism Disorder in a Mouse Model of Type Ⅱ Diabetes Mellitus [J]. Journal of Tea Science, 2016, 36(3): 250-260.
[5]	HU Jinfang, WANG Genbei, XU Yan, MA Xiaohui, LI Changwen, SHEN Xiuping. The Study of Hypoglycemic Effect of Pu′er Tea Extract Combined with Pioglitazone [J]. Journal of Tea Science, 2015, 35(2): 158-164.
[6]	LI Zhiwen, WANG Yuanyuan, WANG Genbei, CHEN Yanfang, LI Changwen, MA Xiaohui. Study of the Hypoglycemic Effect of Pu'er Tea and Green Tea Extracts [J]. Journal of Tea Science, 2014, 34(5): 428-434.
[7]	WANG Dong-feng, WANG Lin-ge, ZHANG Li, XU Ying, Bucheli Peter. Effect of Green Tea on Diabetes [J]. Journal of Tea Science, 2010, 30(4): 243-250.
[8]	LIANG Yan, WANG Yue-fei, XIE Zheng-zhen, ZHANG Xing-hai, YANG Xian-qiang. Research on the Effect of Tea and Mulberry Mixture Tea Bag on Blood Glucose-decrease in Diabetic Model Mice [J]. Journal of Tea Science, 2008, 28(5): 358-362.
[9]	LIANG Jin, ZHANG Jian-yun, CUI Ying-ying, HUANG Long-quan. Study on Chemical Modification and Anticoagulant Function in vitro of Tea Polysaccharides [J]. Journal of Tea Science, 2008, 28(3): 166-171.
[10]	YIN Xue-zhe, XU Hui-xian, JIN Hua. Study of Green Tea Polysaccharides on Lipid Peroxidation of Plasma Lipoproteins [J]. Journal of Tea Science, 2007, 27(4): 299-301.
[11]	ZHOU Xiao-ling, WANG Dong-feng*, LI Su-zhen, ZHOU Xun, HOU Yang-feng, WANG Yuan-hong. Effects of Different Extraction Processes on the Components of Tea Polysaccharides [J]. Journal of Tea Science, 2007, 27(1): 27-32.
[12]	LI Lei, WANG Dong-feng, ZHOU Xiao-ling, DING Qing-bo. Study on Food Functionality of Tea Polysaccharides [J]. Journal of Tea Science, 2006, 26(2): 102-107.
[13]	YIN Jun-feng, YUAN Hai-bo, GU Ji-ping, LIN Zhi-zhong, CHEN Jian-xin, TAN Jun-feng, WANG Fang. Study on Separation and Concentration of Tea Polysaccharides by Membrane System [J]. Journal of Tea Science, 2006, 26(2): 108-111.
[14]	DING Ren-feng, HE Pu-ming, JIE Guo-liang. Study on the Hypoglycemic Mechanism of Tea Polysaccharides and Tea Polyphenols [J]. Journal of Tea Science, 2005, 25(3): 219-224.
[15]	GONG Fa-yong, QI Gui-nian, LI Jing. Study on the Optimum Conditions in Extracting Polysaccharides in Sichuan Brick Tea [J]. Journal of Tea Science, 2005, 25(3): 229-236.