一种茶枝致病菌的分离鉴定及致病力分析

doi:10.13305/j.cnki.jts.2019.06.005

Abstract

Abstract: The pathogens of diseased tea branches in tea garden of Datong town, Jiande city were identified by morphological and molecular methods. Two fungal pathogens were identified, namely Fusarium incarnatum and Colletotrichum fructicola. F. incarnatum here was reported as the pathogen of tea trees. Leaves and stems of Longjing43 and Zhongcha108 were used in the experiment to analyze the pathogenicity of the two pathogens. The results show that F. incarnatum had strong pathogenicity to young leaves and stems of Longjing43, indicating it is the main pathogen causing relative disease. F. incarnatum and C. fructicola might infect tea leaves together which needs more attention in tea disease recognition, control and prevention in future.

Key words: morphological identification, molecular identification, tea branch, Fusarium, pathogenicity

CLC Number:

ZHANG Lan, GUO Huawei, LI Menghan, LIAO-YANG Wenke, LI Xin, HAN Wenyan. Identification and Pathogenicity Analysis of Pathogens in Tea Branches[J]. Journal of Tea Science, 2019, 39(6): 661-668.

References 26

[1]	杨亚军. 中国茶树栽培学[M]. 上海: 上海科学技术出版社, 2005: 37.
[2]	陈宗懋, 陈雪芬. 茶树病害的诊断和防治[M]. 上海: 上海科学技术出版社, 1990.
[3]	唐美君, 肖强. 茶树病虫及天敌图谱[M]. 北京: 中国农业出版社, 2018: 2-15.
[4]	王振华. 一种新茶树病害鉴定及其检测技术研究[D]. 武汉: 华中农业大学, 2017: 9-10.
[5]	Schaad N W, Frederick R D, Shaw J.et al.Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues[J]. Annual Review of Phytopathology, 2003, 41(1): 305-324.
[6]	Vincelli P, Tisserat N.Nucleic acid based pathogen detection in applied plant pathology[J]. Plant Disease, 2008, 92(5): 660-669.
[7]	彭成彬, 柯栋贤, 陈美霞, 等. 茶树镰刀菌的分离鉴定和遗传多样性研究[J]. 中国茶叶, 2018, 40(8): 27-32.
[8]	Carbone I, Kohn L M.A method for designing primer sets for speciation studies in filamentous ascomycetes[J]. Mycologia, 1999, 91(3): 553-556.
[9]	王玉春. 中国茶树炭疽菌系统发育学研究及茶树咖啡碱抗炭疽病的作用[D]. 杨凌: 西北农林科技大学, 2016: 21-33.
[10]	王燕, 王春伟, 王琳, 等. 甜瓜镰刀菌果腐病新病原菌Fusarium incarnatum的鉴定及生物学特性[J]. 园艺学报, 2019, 46(3): 529-539.
[11]	单柳颖. 引起玉米茎腐病的镰孢菌的分离鉴定与多样性分析[D]. 北京: 中国农业科学院, 2017: 17-24.
[12]	Huang L, Yang J Y, Zhu Y N, et al.Canker on culm of Bambusa multiplex (Lour.) Raeusch. ex Schult. caused by Fusarium incarnatum (Roberge) Sacc.[J]. Journal of Phytopathology, 2019, 167(2): 91-97.
[13]	Shen H F, Jiang S B, Lin B R, et al.First report of fruit spot of Toxicodendron sylvestre caused by Fusarium incarnatum in China[J]. Plant Disease, 2019, 103(2): 376. DOI: 10.1094/PDIS-07-18-1290-PDN.
[14]	夏雨晨, 朱永兴, 马东方, 等. 小麦赤霉病菌拮抗菌的分离及鉴定[J]. 江西农业大学学报, 2019, 41(1): 33-42.
[15]	陈宏州, 杨红福, 姚克兵, 等. 水稻恶苗病病原菌鉴定及室内药剂毒力测定[J]. 植物保护学报, 2018, 45(6): 1356-1366.
[16]	刘一景. 蔬菜枯萎病镰刀菌种类研究[J]. 现代园艺, 2018, 13(7): 13-16.
[17]	龙盛风, 李文教, 韦绍龙, 等. 不同种植模式下甘蔗梢腐病和香蕉枯萎病病害调查及其病原真菌分离鉴定[J]. 南方农业学报, 2019, 50(2): 292-298.
[18]	王丽波, 崔玥晗, 岳玲, 等. 百合枯萎病的研究进展[J]. 园艺与种苗, 2018, 38(12): 50-53.
[19]	马英玲, 韦春义, 林红兵. 油茶幼苗根腐病病原及防效研究[J]. 广东农业科学, 2013, 40(19): 70-71.
[20]	赖传雅, 赖传碧, 曾凡凯, 等. 茶扦插苗根腐性苗枯病菌茄镰孢霉专化型研究及相关问题的商榷[J]. 云南农业大学学报, 自然科学版, 2012(5): 665-669.
[21]	Sinniah G D, Munasinghe C E, Mahadevan N, et al.Recent incidence of collar canker and dieback of tea (Camellia sinensis) caused by Fusarium solani species complex in Sri Lanka[J]. Australasian Plant Disease Notes, 2017, 12(1): 41. DOI: 10.1007/s13314-017-0262-5.
[22]	Phong N H, Pongnak W, Soytong K.Antifungal activities of Chaetomium spp. against Fusarium wilt of tea[J]. Plant Protection Science, 2016, 52(1): 10-17.
[23]	张永乐. 山东省茶树主要叶部病害病原鉴定及生物学特性研究[D]. 泰安: 山东农业大学, 2018.
[24]	刘威. 茶树炭疽病的病原鉴定及其遗传多样性分析[D]. 福州: 福建农林大学, 2013.
[25]	向梅梅, 张云霞, 刘霄. 炭疽菌属真菌分类的研究进展[J]. 仲恺农业工程学院学报, 2017, 29(4): 1-8.
[26]	刘威, 叶乃兴, 陈玉森, 等. 茶树炭疽菌Colletotrichum fructicola的鉴定及系统发育分析[J]. 茶叶科学, 2014, 34(1): 95-104.

Identification and Pathogenicity Analysis of Pathogens in Tea Branches

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