茶树中葡萄糖-6-磷酸脱氢酶基因（CsG6PDHs）的克隆与表达分析

doi:10.13305/j.cnki.jts.20210622.002

Abstract

Abstract: As a key rate-limiting enzyme in pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PDH, EC1.1.1.49) plays an important role in plant growth and development, and also in responding to environmental stresses. However, the function of G6PDH in tea plants has not yet been reported. In our study, 3 G6PDH genes were cloned from tea plant, named as CsG6PDH1 (MW025829), CsG6PDH2 (MW025830) and CsG6PDH4 (MW025831), respectively. Phylogenetic analysis shows that CsG6PDH1 and CsG6PDH4 belong to plastid protein, while CsG6PDH2 belongs to cytoplasmic protein. Quantitative analysis shows that the expressions of CsG6PDHs varied in different tissues. Under cold or Colletotrichum infection treatments, the time course expressions of both CsG6PDH1 and CsG6PDH4 were gradually reduced. Besides, both CsG6PDH1 and CsG6PDH4 were induced by cold acclimation in different tea cultivars. In addition, the expressions of CsG6PDHs were up-regulated during bud dormancy and flush periods. Consequently, our results implicate that CsG6PDHs are widely participated in tea plant growth and development, and also involved in responding to abiotic and biotic stresses. This study provided a theoretical basis for in-depth study of the function of CsG6PDHs in tea plants.

Key words: glucose-6-phosphate dehydrogenase, Camellia sinensis, expression analysis, cold, cold acclimation

CLC Number:

WANG Yanding, WANG Huan, LI Nana, WANG Lu, HAO Xinyuan, WANG Yuchun, DING Changqing, YANG Yajun, WANG Xinchao, QIAN Wenjun. Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis[J]. Journal of Tea Science, 2021, 41(4): 497-510.

References

[1] Pickl A, Schönheit P.The oxidative pentose phosphate pathway in the haloarchaeon Haloferax volcanii involves a novel type of glucose-6-phosphate dehydrogenase: the archaeal Zwischenferment[J]. FEBS Letters, 2015, 589(10): 1105-1111.
[2] Stanton R C.Glucose-6-phosphate dehydrogenase, NADPH, and cell survival[J]. Iubmb Life, 2012, 64(5): 362-369.
[3] 于定群, 汤浩茹, 张勇, 等. 高等植物葡萄糖-6-磷酸脱氢酶的研究进展[J]. 生物工程学报, 2012, 28(7): 800-812.
Yu D Q, Tang H R, Zhang Y, et al.Research progress in glucose-6-phosphate dehydrogenase in higher plants[J]. Chinese Journal of Biotechnology, 2012, 28(7): 800-802.
[4] Dangthu Q, Jang S H, Lee C W.Biochemical comparison of two glucose 6-phosphate dehydrogenase isozymes from a cold-adapted Pseudomonas mandelii[J]. Extremophiles, 2020, 24(49): 501-509.
[5] Graeve K, von Schaewen A, Scheibe R, et al. Purification, characterization, and cDNA sequence of glucose-6-phosphate dehydrogenase from potato (Solanum tuberosum L.)[J]. The Plant Journal, 1994, 5(3): 353-361.
[6] Krepinsky K, Karsten M, Martin W, et al.Purification and cloning of chloroplast 6-phosphogluconate dehydrogenase from spinach[J]. European Journal of Biochemistry, 2001, 268(9): 2678-2686.
[7] Bailey-Serres J, Nguyen M T.Purification and characterization of cytosolic 6-phosphogluconate dehydrogenase isozymes from maize[J]. Plant Physiology, 1992, 100(3): 1580-1583.
[8] Zhao Y, Cui Y F, Huang S Y.Genome-wide analysis of the glucose-6-phosphate dehydrogenase family in soybean and functional identification of GmG6PDH2 involvement in salt stress[J]. Frontiers in Plant Science, 2011, 11: 214. doi: 10.3389/fpls.2020.00214.
[9] Wakao S, Benning C.Genome-wide analysis of glucose-6-phosphate dehydrogenases in Arabidopsis[J]. The Plant Journal, 2005, 41(2): 243-256.
[10] 丁林云. 水稻胞质6-磷酸葡萄糖酸脱氢酶基因的功能分析[D]. 南京: 南京农业大学, 2007.
Ding L Y.Functional analysis of cytoplast 6-phosphogluconate dehydrogenase in pentose phosphate pathway from rice (Oryza sativa L.) [D]. Nanjing: Nanjing Agricultural University, 2007.
[11] 田宇, 彭瞰看, 宋春华, 等. 小麦G6PDH基因的生物信息学分析及其低温胁迫下苗期的表达特征[J]. 麦类作物学报, 2019, 39(6): 631-638.
Tian Y, Peng K K, Song C H, et al.Bioinformatics analysis of wheat G6PDH genes and their expression patterns in tillering node and leaf under cold stress[J]. Journal of Triticeae Crops, 2019, 39(6): 631-638.
[12] 张心菲. 赤桉葡萄糖-6-磷酸脱氢酶(G6PDH)基因家族的表达与功能分析[D]. 广州: 华南农业大学, 2016.
Zhang X F.Expression and function analysis of glucose-6-phosphate dehydrogenase gene family EcG6PDH in Eucalyptus camaldulensis [D]. Guangzhou: South China Agricultural University, 2016.
[13] Sindelár L, Sindelárová M.Correlation of viral RNA biosynthesis with glucose-6-phosphate dehydrogenase activity and host resistance[J]. Planta, 2002, 215(5): 862-869.
[14] Wang X M, Ma Y Y, Huang C H, et al.Involvement of glucose-6-phosphate dehydrogenase in reduced glutathione maintenance and hydrogen peroxide signal under salt stress[J]. Plant Signaling and Behavior, 2008, 3(6): 394-395.
[15] Landi S, Nurcato R, De Lillo A, et al.Glucose-6-phosphate dehydrogenase plays a central role in the response of tomato (Solanum lycopersicum) plants to short and long-term drought[J]. Plant Physiology and Biochemistry, 2016, 105: 79-89.
[16] 王友须. 荷花葡萄糖-6-磷酸脱氢酶基因克隆及其在响应水淹胁迫中的功能分析[D]. 南京: 南京农业大学, 2016.
Wang Y X.Glucose-6-phosphate dehydrogrnase gene cloning and it is role in lotus response to submerge [D]. Nanjing: Nanjing Agricultural University, 2016.
[17] Lin Y Z, Lin S Z, Zhang W.Cloning and sequence analysis of a glucose-6-phosphate dehydrogenase gene PsG6PDH from freezing-tolerant Populus suaveolens[J]. Forestry Studies in China, 2015, 7(1): 1-6.
[18] Wang X M, Ma Y Y, Huang C H, et al.Glucose-6-phosphate dehydrogenase plays a central role in modulating reduced glutathione levels in reed callus under salt stress[J]. Planta, 2008, 227(3): 611-623.
[19] Scharte J, Schön H, Tjaden Z, et al.Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(19): 8061-8066.
[20] Qian W J, Yue C, Wang Y C, et al.Identification of the invertase gene family (INVs) in tea plant and their expression analysis under abiotic stress[J]. Plant Cell Reports, 2016, 35(11): 2269-2283.
[21] Qian W J, Xiao B, Wang L, et al.CsINV5, a tea vacuolar invertase gene enhances cold tolerance in transgenic Arabidopsis[J]. BMC Plant Biology, 2018, 18(1): 228-248.
[22] Yue C, Cao H L, Wang L, et al.Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season[J]. Plant Molecular Biology, 2015, 86(6): 591-608.
[23] Wang Y C, Qian W J, Li N N, et al.Metabolic changes of caffeine in tea plant (Camellia sinensis (L.) O. Kuntze) as defense response to Colletotrichum fructicola[J]. Journal of Agricultural and Food Chemistry, 2016, 64(35): 6685-6693.
[24] 钱文俊, 岳川, 曹红利, 等. 茶树中性/碱性转化酶基因CsINV10的克隆与表达分析[J]. 作物学报, 2016, 42(3): 376-388.
Qian W J, Yue C, Cao H L, et al.Cloning and expression analysis of a neutral/alkaline invertase gene (CsINV10) in tea plant (Camellia sinensis L. O. Kuntze)[J]. Acta Agronomica Sinica, 2016, 42(3): 376-388.
[25] Hao X Y, Horvath D P, Chao W S, et al.Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plant (Camellia sinensis (L.) O. Kuntze)[J]. International Journal of Molecular Sciences, 2014, 15(12): 22155-22172.
[26] 林元震, 张志毅, 林善枝, 等, 桉树葡萄糖-6-磷酸脱氢酶(G6PDH)的全基因组分析与进化研究[J]. 安徽农业科学, 2011, 39(29): 17804-17805, 17906.
Lin Y Z, Zhang Z Y, Lin S Z, et al.Genome-wide analysis of glucose-6-phosphate dehydrogenase (G6PDH) and its evolution in Eucalyptus grandsis[J]. Journal of Anhui Agricultural Sciences, 2011, 39(29): 17804-17805, 17906.
[27] Long X Y, He B, Fang Y J, et al, Identification and characterization of the glucose-6-phosphate dehydrogenase gene family in the para rubber tree, Hevea brasiliensis[J]. Frontiers in Plant Science, 2016, 7: 215. doi: 10.3389/fpls.2016.00215.
[28] 王晓敏. 葡萄糖-6-磷酸脱氢酶在芦苇愈伤组织盐适应性中调节作用的机理研究[D]. 兰州: 兰州大学, 2008.
Wang X M.Studies on the mechanism of regulations of glucose-6-phosphate dehydrogenase in the adaptation of reed callus to salt stress [D]. Lanzhou: Lanzhou University, 2008.
[29] Cappellini M D, Montemuros di F M, Dotti C, et al. Molecular characterisation of the glucose-6-phosphate dehydrogenase (G6PD) Ferrara II variant[J]. Human Genetics, 1995, 95(4): 440-442.
[30] 王晓晖, 刘晓, 高博闻, 等. 腊梅葡萄糖-6-磷酸脱氢酶(G6PDH1)基因的克隆及表达分析[J]. 中国中药杂志, 2015, 40(21): 4160-4164.
Wang X H, Liu X, Gao B W, et ai. Cloning and expression analysis of glucose-6-phosphate dehydrogenase 1 (G6PDH1) gene from Chimonanthus praecox[J]. China Journal of Chinese Materia Medica, 2015, 40(21): 4160-4164.
[31] 朱守晶, 史文娟. 苎麻镉响应基因BnG6PDH1的克隆和表达分析[J]. 江苏农业学报, 2019, 35(2): 262-270.
Zhu S J, Shi W J.Cloning and expression analysis of cadmium-responsive gene BnG6PDH1 from ramie (Boehmeria nivea L.)[J]. Jiangsu Journal of Agricultural Sciences, 2019, 35(2): 262-270.
[32] 林元震. 甜杨葡萄糖6-磷酸脱氢酶基因克隆及结构分析与功能鉴定[D]. 北京: 北京林业大学, 2006.
Lin Y Z.Gene cloning, structure analysis and function identification of glucose-6-phosphate dehydrogenase from Populus suaveolens [D]. Beijing: Beijing Forestry University, 2006.
[33] Tupy J.The level and distribution pattern of fatex sucrose along the trunk of Hevea brasiliensis Mull. arg. as affected by the sink region induced by latex tapping[J]. Physiology, 1973, 11: 1-11.
[34] Wakao S, Andre C, Benning C.Functional analyses of cytosolic glucose-6-phosphate dehydrogenases and their contribution to seed oil accumulation in Arabidopsis[J]. Plant Physiology, 2008, 146(1): 277-288.
[35] Lin S Z, Zhang Z Y, Liu W F, et al.Role of glucose-6-phosphate dehydrogenase in freezing-induced freezing resistance of Populus suaveolens[J]. Acta Photophysiologica Sinica, 2005, 31(1): 34-40.
[36] 曹玉芳. 青檀抗寒性的初步研究[D]. 北京: 北京林业大学, 2005.
Cao Y F.Preliminary studies on freezing resistance of Pteroceltis tatarinowii [D]. Beijing: Beijing Forestry University, 2005.
[37] Hao X, Yang Y, Yue C, et al.Comprehensive transcriptome analyses reveal differential gene expression profiles of Camellia sinensis axillary buds at para-, endo-, ecodormancy, and bud flush stages[J]. Frontiers in Plant Science, 2017, 8:553. doi: 10.3389/fpls.2017.00553.
[38] 浦心春, 韩建国. 结缕草种子打破休眠过程中的代谢调控[J]. 中国草地学报, 1994(3): 20-24.
Pu X C, Han J G.Metabolic control of zoysiagrass seed when breaking dormancy[J]. Chinese Journal of Grassland, 1994(3): 20-24.

[1]	. [J]. Journal of Tea Science, 1965, 2(04): 59 -63 .
[2]	. [J]. Journal of Tea Science, 1965, 2(03): 18 -21 .
[3]	. [J]. Journal of Tea Science, 1965, 2(03): 22 -24 .
[4]	. [J]. Journal of Tea Science, 1966, 3(02): 55 -57 .
[5]	. [J]. Journal of Tea Science, 1966, 3(02): 82 -83 .
[6]	. [J]. Journal of Tea Science, 1986, 6(01): 1 -8 .
[7]	. [J]. Journal of Tea Science, 1987, 7(02): 29 -34 .
[8]	. [J]. Journal of Tea Science, 1988, 8(01): 37 -42 .
[9]	. [J]. Journal of Tea Science, 1995, 15(02): 154 .
[10]	. [J]. Journal of Tea Science, 1999, 19(01): 73 -76 .

Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis

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