L-茶氨酸对CUMS抑郁大鼠海马和肠道损伤的干预作用研究

doi:10.13305/j.cnki.jts.2021.04.005

Abstract

Abstract: Using SPF-grade SD male rats as experimental animals, a rat model of chronic unpredictable mild stress (CUMS) was established and different doses of L-theanine were given intragastrically for intervention. Then, the effects of L-theanine on the depression-like behaviors, histopathological changes and the levels of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), 5-hydroxytryptamine (5-HT) and glucagon-like peptide 1 (GLP-1) contents of hippocampus and gut were observed and evaluated, which aimed to explore the intervention effects of L-theanine on the structural damage and dysfunction of hippocampus and gut in CUMS depressed rats. The results show that, compared with the model group, pretreatment with different doses of L-theanine improved the body weight,sugar-water preference, number of crossing and rearing, activity in water, number of entering the light box and the duration time of ratsat different degrees, especially the middle-dose group (100 mg·kg^-1) reached a significant level. L-theanine effectively repaired hippocampal neuron damage caused by CUMS, reduced IL-6 and TNF-α levels in serum, colon and cecum and attenuated the damage and inflammation degree of the colon and cecum tissues, and the middle-dose group also exhibited better effect. L-theanine could increase the contents of 5-HT and GLP-1 in hippocampus, cerebral cortex and colon of CUMS rats, and reached significant levels under some conditions. These results indicate that L-theanine can effectively improve the depression-like behavior, structural damage and dysfunction of hippocampus and gut in CUMS model rats, and its regulation of ‘gut-brain axis’ may be the action mechanism of anti-depression.

Key words: L-theanine, depression-like behavior, hippocampus, gut damage, gut-brain axis

CLC Number:

S571
R749.4

CHEN Meiyan, LIU Fen, LIN Yong, ZUO Gaolong, ZUO Yingpeng, LIU Zhonghua. Intervention Effects of L-Theanine on the Damage of Hippocampus and Gut in CUMS Depressed Rats[J]. Journal of Tea Science, 2021, 41(4): 511-524.

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Intervention Effects of L-Theanine on the Damage of Hippocampus and Gut in CUMS Depressed Rats

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