茶谷蛾成虫触角转录组及嗅觉相关基因分析

doi:10.13305/j.cnki.jts.20210316.001

Abstract

Abstract: To identify the olfactory-related genes, the transcriptome analysis of the Agriophara rhombata was performed using Illumina HiSeq 4000. Through sequence alignment, 16 027 unigenes were annotated in NR databases. Totally 11 701 unigenes were annotated in GO database, which can be divided into 3 groups according to their functions: cellular component, molecular function and biological process. Totally 6 047 unigenes were further annotated and divided into 25 functional groups in KOG databases. KEGG pathway analysis shows that 12 009 unigenes were annotated into 283 metabolism pathways. According to the annotation information, 238 candidate olfactory-related genes were obtained including 108 odorant binding protein (OBP) genes, 55 odorant /olfactory receptor (OR) genes, 26 gustatory receptor (GR) genes, 25 ionotropic receptor (IR) genes, 11 chemosensory protein (CSP) genes, 4 sensory neuron membrane protein (SNMP) genes, 4 sensory perception (SP) genes , 4 chemosensory receptor (CR) genes and an odorant degrading enzyme (ODE) gene. It was found that 12 odorant binding protein genes, 9 odorant/olfactory receptor genes, 4 pheromone binding protein genes, 3 gustatory receptor genes, chemosensory protein gene and 1ionotropic receptor gene were differentially expressed in the antenna of Agriophara rhombata. In this study, the identification of candidate olfactory-related genes laid a molecular foundation for further studies on gene function and olfactory perception mechanism of the Agriophara rhombata.

Key words: Agriophara rhombata, antennal transcriptome, high-throughput sequencing, genome annotation, olfaction-related genes

CLC Number:

LONG Yaqin, LUO Ziwen, WANG Xuesong, LONG Lixue, YU Xiangshuai, LI Jinlong, QU Hao, WANG Yungang, CHEN Linbo. Analysis of the Antennal Transcriptome and Olfactory-related Genes in the Agriophara rhombata[J]. Journal of Tea Science, 2021, 41(4): 553-563.

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Analysis of the Antennal Transcriptome and Olfactory-related Genes in the Agriophara rhombata

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