茶树CsbHLH024和CsbHLH133转录因子功能鉴定

doi:10.13305/j.cnki.jts.2022.03.001

Abstract

Abstract: Tea plant leaf trichomes contain various secondary metabolites and play an important role in the tea appearance quality as well as the response of tea plants to biotic and abiotic stresses. In this study, the function of leaf trichome-related genes CsbHLH024 and CsbHLH133 were analyzed using Bimolecular Fluorescent Complimentary (BiFC), GUS staining and overexpression experiments. The results show that CsbHLH024/CsbHLH133 and CsTTG1 could interact in plants, and their promoters could drive downstream gene expression in leaf tissues. They were further transformed into wild Arabidopsis thaliana (Col) and corresponding homozygous mutants, respectively to get overexpression lines. Both genes could affect the leaf trichome formation in Arabidopsis thaliana, restore the phenotype of the mutants, and induce the expression levels of trichome-related genes. This study provided a theoretical basis for further research on the molecular regulation mechanism of trichome formation in tea leaves.

Key words: tea plant, trichome formation, CsbHLH024, CsbHLH133, molecular regulation

CLC Number:

S571.1

LIU Renjian, WANG Yuyuan, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants[J]. Journal of Tea Science, 2022, 42(3): 347-357.

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Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants

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