超高效液相色谱-串联质谱法检测绿茶中16种农药残留

doi:10.13305/j.cnki.jts.20220416.004

Abstract

Abstract: An analytical method was developed for the simultaneous determination of 16 pesticide residues in green tea by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The target pesticides in tea samples were extracted by acetonitrile with 1% formic acid, cleaned up by a TPT-SPE column, ionized in electrospray positive ionization source (ESI⁺) mode, and scanned for determination of the target parent and daughter ions by mass spectrometry in multiple reaction monitoring mode (MRM). The results show that the target pesticides were linear in the range of 0.005-1.000 mg·kg^-1 with the correlation coefficient R²>0.992 6. The average recoveries ranged from 74.0% to 105.4% at the four spiked levels of 0.010, 0.050, 0.100、1.000 mg·kg^-1, and the relative standard deviations (RSD) were <20.0%. The limits of quantification were 10-50 μg·kg^-1. The method is fast and sensitive, and the technical parameters are in accordance with the relevant domestic and international regulations and standards. And it can meet the requirements of simultaneous determination of various pesticide residues in green tea.

Key words: multi-residue of pesticides analysis, UPLC-MS/MS, TPT-SPE column clean, green tea

CLC Number:

S571.1

LI Ziqiang, YANG Mei, ZHANG Xinzhong, LUO Fengjian, ZHOU Li, LOU Zhengyun, SUN Hezhi, WANG Xinru, CHEN Zongmao. Residue Determination of Sixteen Pesticides in Green Tea by UPLC-MS/MS[J]. Journal of Tea Science, 2022, 42(4): 537-548.

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Residue Determination of Sixteen Pesticides in Green Tea by UPLC-MS/MS

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