不同种质茶叶籽皂素含量及组成分析

doi:10.13305/j.cnki.jts.2022.05.005

Abstract

Abstract: Tea saponins are abundant in the seeds of Camellia sinensis with specific biological activities. Their contents and composition in tea seeds of different germplasms remain unclear. In this study, seeds from 21 tea germplasms were collected from the same region of Zhejiang and used as experimental materials to determine the basic characteristics, saponin contents and composition. Correlation analysis between germplasm and saponin composition was also performed. The results show that the seed weight, shell kernel ratio and saponin content of different tea seeds were significantly different (P<0.05). The saponin content detected by UV spectral method and HPLC ranged from 30.82% to 48.16% and 16.93% to 31.82%, respectively. ‘Huangguanyin' had the highest saponin content in tea seeds. Totally 68 tea seed saponin monomers were detected simultaneously using ultrahigh performance liquid chromatography-quadrupole orbitrap mass spectrometer（UHPLC-Q-Exactive/MS）. Theasaponin E1 had the highest relative intensity. Orthogonal partial least squares discriminant analysis (OPLS-DA) shows that the 21 tea seed germplasms can be distinguished into 2 groups according to tree type with 21 different Camelliasaponin B1/B2, Theasaponin A5/A6, Camelliasaponin C1/C2 and Assamsaponin G were the most significant characteristic substances of semi-tree form and arbor form resources. The results of the study laid the foundation for the selection and value-added utilization of tea seed saponins.

Key words: tea seed, saponin, monomer, UHPLC-Q-Exactive/MS

CLC Number:

CHEN Yuhong, GAO Ying, HAN Zhen, YIN Junfeng. Analysis of the Saponin Contents and Composition in Tea Seeds of Different Germplasms[J]. Journal of Tea Science, 2022, 42(5): 705-716.

References

[1] 马跃青, 张正竹. 茶叶籽综合利用研究进展[J]. 中国油脂, 2010, 35(9): 66-69.
Ma Y Q, Zhang Z Z.Research advance in comprehensive utilization of tea seeds[J]. China Oils and Fats, 2010, 35(9): 66-69.
[2] Yang W S, Ko J, Kim E, et al.21-O-angeloyltheasapogenol E3, a novel triterpenoid saponin from the seeds of tea plants, inhibits macrophage-mediated inflammatory responses in a NF-κB-dependent manner[J]. Mediators of Inflammation, 2014, 2014: 658351. doi: 10.1155/2014/658351.
[3] Yang H, Cai R, Kong Z Y, et al.Teasaponin ameliorates murine colitis by regulating gut microbiota and suppressing the immune system response[J]. Frontiers in Medicine, 2020, 7: 584369. doi: 10.3389/fmed.2020.584369.
[4] Yang H, Shao X, Yu G H.Effect of tea saponin on blood pressure in spontaneously hypertensive rats[J]. Chinese Journal of Clinical Healthcare, 2007, 116(5): 388-395.
[5] Yong Y, Chen X, Xing H.Hypolipidemic and antioxidant activities of hydrolyzed saponins from defatted seeds of Camellia oleifera Abel[J]. Lation American Journal of Pharmacy, 2013, 32(3): 409-417.
[6] Khan M I, Ahhmed A, Shin J H, et al.Green tea seed isolated saponins exerts antibacterial effects against various strains of gram positive and gram negative bacteria, a comprehensive study in vitro and in vivo[J]. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 2018: 3486106. doi: 10.1155/2018/3486106.
[7] Morikawa T, Li N, Nagatomo A, et al.Triterpene saponins with gastroprotective effects from tea seed (the seeds of Camellia Sinensis)[J]. Journal of Natural Products, 2006, 69(2): 185-190.
[8] 赵世明. 茶皂素的化学结构及药理活性研究[J]. 国外医药(植物药分册), 1998, 13(1): 3-6.
Zhao S M.Study on the chemical structure and pharmacological activity of tea saponin[J]. World Phytomedicines, 1998, 13(1): 3-6.
[9] Guo N, Tong T T, Ren N, et al.Saponins from seeds of Genus Camellia[J]. Phytochemistry and Bioactivity, 2018, 149: 42-55.
[10] Wu X J, Jia L Y, Wu J F, et al.Simultaneous determination and quantification of triterpene saponins from Camellia sinensis seeds using UPLC-PDA-QTOF-MS/MS[J]. Molecules, 2019, 24(20): 3794. doi: 10.3390/molecules24203794.
[11] Chen C, Zhu H Q, Kang J X, et al.Comparative transcriptome and phytochemical analysis provides insight into triterpene saponin biosynthesis in seeds and flowers of the tea plant (Camellia sinensis)[J]. Metabolites, 2022, 12(3): 204. doi: 10.3390/metabo12030204.
[12] Chen Y F, Yang C H, Chang M S, et al.Foam properties and detergent abilities of the saponins from Camellia oleifera[J]. International Journal of Molecular Sciences, 2010, 11: 4417-4425.
[13] 谭搏, 曹福祥, 赵莹. 油茶饼中茶皂素的定量分析[J]. 精细化工中间体, 2009, 39(2): 67-69.
Tan B, Cao F X, Zhao Y.Quantitative analysis of tea saponin in Camellia cake[J]. Fine Chemical Intermediates, 2009, 39(2): 67-69.
[14] 吴学进. 茶籽皂素的分离鉴定与定量检测[D]. 杭州: 浙江大学, 2018.
Wu X J.Separation, identification and quantification of triterpene saponins in seeds of Camellia sinensis [D]. Hangzhou: Zhejiang University, 2018.
[15] 郭华. 高档茶籽油的提取及茶籽综合利用技术研究[D]. 长沙: 湖南农业大学, 2007.
Guo H.Study of technologies on refining high quality tea seed oil and comprehensively utilizing tea seed [D]. Changsha: Hunan Agricultural University, 2007.
[16] Cui C J, Zong J F, Sun Y, et al.Triterpenoid saponins from the genus Camellia: structures, biological activities, and molecular simulation for structure-activity relationship[J]. Food & Function, 2018, 9(6): 3069-3091.
[17] 王羚, 方学智. 油茶皂素定量分析方法研究进展[J]. 食品工业, 2020, 41(11): 278-282.
Wang L, Fang X Z.Research progress in quantitative analysis of tea saponin[J]. The Food Industry, 2020, 41(11): 278-282.
[18] 陈莹, 刘松柏, 何良兴, 等. 油茶籽粕和茶皂素中皂苷的定量检测方法研究[J]. 中国粮油学报, 2012, 27(2): 105-111.
Chen Y, Liu S B, He L X, et al.Quantitative analysis of saponin in Camellia seed cake and tea saponins[J]. Journal of the Chinese Cereals and Oils, 2012, 27(2): 105-111.
[19] 张团结, 熊道陵, 许光辉, 等. 油茶籽饼中茶皂素定量检测方法研究[J]. 食品工业科技, 2016, 37(4): 53-56.
Zhang T J, Xiong D L, Xu G H, et al.Quantitative analysis of tea saponin from seed cake[J]. Science and Technology of Food Industry, 2016, 37(4): 53-56.
[20] 赵敬娟, 杜先锋. 油茶皂苷对照品制备及高效液相色谱定量法的研究[J]. 中国油脂, 2009, 34(4): 68-72.
Zhao J J, Du X F.Sasanquasaponin reference substance preparation and quantitative[J]. China Oils and Fats, 2009, 34(4): 68-72.
[21] Morikawa T, Lee I J, Okugawa S, et al.Quantitative analysis of catechin, flavonoid, and saponin constituents in “tea flower”, the flower buds of Camellia sinensis, from different regions in Taiwan[J]. Natural Product Communications, 2013, 11(8): 1553-1557.
[22] Fan L M, He Y F, Xu Y J, et al.Triterpenoid saponins in tea (Camellia sinensis) plants: biosynthetic gene expression, content variations, chemical identification and cytotoxicity[J]. International Journal of Food Sciences and Nutrition, 2021, 72(3): 308-323.
[23] 罗祖良, 张凯伦, 马小军, 等. 三萜皂苷的合成生物学研究进展[J]. 中草药, 2016, 47(10): 1806-1814.
Luo Z L, Zhang K L, Ma X J, et al.Research progress in synthetic biology of triterpen saponins[J]. Chinese Traditional and Herbal Drugs, 2016, 47(10): 1806-1814.
[24] Zhao J, Li P H, Xia T, et al.Exploring plant metabolic genomics: chemical diversity, metabolic complexity in the biosynthesis and transport of specialized metabolites with the tea plant as a model[J]. Critical Reviews in Biotechnology, 2020, 40(5): 667-688.
[25] Oda K, Matsuda T, Murakami S, et al.Adjuvant and haemolytic activities of 47 saponins derived from medicinal and food plants[J]. Biological Chemistry, 2000, 381(1): 67-74.
[26] Yoshikawa M, Morikawa K, Yamamoto Y, et al.Floratheasaponins A-C, acylated oleanane-type triterpene oligoglycosides with anti-hyperlipidemic activities from flowers of the tea plant (Camellia sinensis)[J]. Journal of Natural Products, 2005, 68(9): 1360-1365.
[27] Matsuda S, Nakamura K, Fujimoto R, et al.Medicinal flowers. XXXI. acylated oleanane-type triterpene saponins, Sasanquasaponins I-V, with antiallergic activity from the flower buds of Camellia sasanqua[J]. Chemical & Pharmaceutical Bulletin, 2010, 58(12): 1617-1621.
[28] Murakami T, Nakamura J, Kageura T, et al.Bioactive saponins and glycosides. XVII. Inhibitory effect on gastric emptying and accelerating effect on gastrointestinal transit of tea saponins: structures of assamsaponins F, G, H, I, and J from the seeds and leaves of the tea plant[J]. Chemical & Pharmaceutical Bulletin, 2000, 48: 1720-1725.
[29] Kitagawa I, Hori T, Motozawa T, et al.Structures of new acylated oleanene-type triterpene oligoglycosides, Theasaponins E1 and E2, from the seeds of tea plant, Camellia sinensis (L.) O. Kuntze[J]. Chemical & Pharmaceutical Bulletin, 1998, 46(12): 1901-1906.

Analysis of the Saponin Contents and Composition in Tea Seeds of Different Germplasms

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	XIANG Jing, LIANG Yuerong, ZHAO Dong, WANG Kairong, LU Jianliang, YUAN Ming'an, ZHENG Xinqiang. Variation of Oil Content in Tea Seed Kernel and Fatty Acid Compositions and Contents in Tea Seed Oil among Different Culitivars and Regions [J]. Journal of Tea Science, 2022, 42(2): 233-248.
[2]	CHEN Mingjie, DU Zhenghua, QIN Jianheng, LI Ruoyu, YU Zhaoxi, GUO Li. Analysis of Characteristics of Lipid Metabolism of Different Germplasm Tea Seeds [J]. Journal of Tea Science, 2021, 41(3): 350-360.
[3]	CHANG Yali, HUANG Xiaobing, JIANG Shuangfeng, HUANG Shuangjie, SUN Mufang, LIU Wei, GUO Guiyi. Analysis of Fat Content and Fatty Acid Composition and Absolute Content in the Tea Seeds from Southern Henan Tea Germplasms [J]. Journal of Tea Science, 2020, 40(3): 352-362.
[4]	JIANG Jinzhong, YANG Pengming, WANG Zibu, MU Ande, WANG Xingchun. Studies on the Identification and Isolation of Lactobacillus plantarum ssp. plantarum JJZ21 and Its Fermentation Properties [J]. Journal of Tea Science, 2018, 38(6): 625-633.
[5]	LI Xuelian, FANG Zhiguo, XIA Huilong. Preliminary Research on Induced Biodegradation of PCBs of Tea Saponin [J]. Journal of Tea Science, 2015, 35(1): 91-96.
[6]	GONG Zhi-hua, LIANG Dan, ZHANG Zhi-bo, XIAO Wen-Jun. Study on Tea Seed Saponin Extraction by Microwave-assisted Method [J]. Journal of Tea Science, 2013, 33(4): 358-363.
[7]	ZHENG De-yong, CHANG Yu-xi, YE Nai-xing, YANG Jiang-fan. Study on the Variation of Important Functional Compositon in the Tea Seeds on its Maturation Period [J]. Journal of Tea Science, 2013, 33(3): 253-260.
[8]	CHANG Yu-xi, ZHENG De-yong, YE Nai-xing, YANG Jiang-fan. Analysis of Fatty Acid Composition in the Seed Oilfrom Fujian Tea Germplasm [J]. Journal of Tea Science, 2012, 32(1): 22-28.
[9]	PENG Zhong-jin, MA Cheng-jin, XIAO Wang, HUANG Ting, ZHENG Jun-xiao, XIANG Yong-ping. Optimization on Processing Parameters for Biodiesel from Tea Seed Oil [J]. Journal of Tea Science, 2012, 32(1): 44-52.
[10]	XIA Xiao-ping, ZHENG Cai-hong, FENG Chun-hong, XIA Chun-hua. Progress in Study on the Chemical Structure and Pharmacological Activities of Triterpene Saponins from Tea Plant (Theaceae) [J]. Journal of Tea Science, 2011, 31(5): 391-398.
[11]	ZHENG Sheng-hong, LI Da-xiang, FANG Shi-hui, TANG Zhi-jing. Production of Xylose from Tea Seed Shellswith Acid Hydrolysis [J]. Journal of Tea Science, 2011, 31(3): 195-200.
[12]	DONG Rui-xia, LI Li-xiang, JIANG Qi-zhong. Identification and Analysis of Procyanidins from Episperm of Tea (Camellia sinensis) Seeds [J]. Journal of Tea Science, 2011, 31(2): 119-123.
[13]	TU Yun-fei, YANG Xiu-fang, KONG Jun-hao. Combination of Both Basic Water Saturated Butanol Extraction System and Macroporous Resin for Purification of Tea-Saponin [J]. Journal of Tea Science, 2010, 30(S1): 556-560.
[14]	MA Rong-chao, LI Zhang-lin, WU Wei-xiong. Experimental Analysis of Combustion Gas of Tea-seed Oil [J]. Journal of Tea Science, 2009, 29(5): 402-406.
[15]	DONG Dao-qing, CHEN Jian-ming, YU Xiao-ping, CHEN Lie-zhong, ZHANG Jue-feng. Synergistic Effect of Tea Saponin on Tripterygium wilfordii EC Against Larvae of Geometrid Ectropis obliqua Prout [J]. Journal of Tea Science, 2008, 28(3): 228-233.