一种基于儿茶素的多酚氧化酶交联聚集体制备及其高效催化合成茶黄素-3,3ʹ-双没食子酸酯研究

doi:10.13305/j.cnki.jts.2023.03.005

Abstract

Abstract: In order to prepare theaflavin more efficiently and economically, catechins were used as the cross-linking reagent in this study, and a polyphenol oxidase (tyrosinase) crossed-linked aggregate derived from Bacillus megaterium was prepared and used for the synthesis of theaflavin-3,3ʹ-digalate. Through optimization of enzyme cross-linking parameters and the catalytic performance before and after cross-linking, the results show that the optimal enzyme activity recovery rate of cross-linked enzyme (200 U·mL^-1) could be obtained at pH 4.0, EGCG 0.5 mg·mL^-1 and cross-linking time 50 min. Compared with free enzymes, cross-linked enzymes showed better catalytic performance (thermal stability, organic solvent tolerance, substrate tolerance). When the cross-linked enzyme was used to synthesize theaflavin-3,3′-digallate, the concentration of the product could reach 800 μg·mL^-1, and the cross-linked enzyme could be reused for at least three batches. This method could significantly reduce the application cost of theaflavin, which has potential industrial application value.

Key words: enzyme cross-linked aggregates, tyrosinase, preparation, theaflavins

CLC Number:

ZHOU Jinghui, LIU Changwei, ZHANG Sheng, XU Gang, XU Wei, HUANG Jian′an, LIU Zhonghua. Preparation of a Cross-linked Polyphenol Oxidase Aggregate Based on Catechins and Its Efficient Catalytic Synthesis of Theaflavin-3,3ʹ-digalate[J]. Journal of Tea Science, 2023, 43(3): 377-388.

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Preparation of a Cross-linked Polyphenol Oxidase Aggregate Based on Catechins and Its Efficient Catalytic Synthesis of Theaflavin-3,3ʹ-digalate

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