草甘膦胁迫对茶树叶片中莽草酸含量的影响

doi:10.13305/j.cnki.jts.2023.05.005

Abstract

Abstract: To investigate the effect of glyphosate stress on the growth and shikimic acid metabolism of tea (Camellia sinensis L.) plants, tea seedlings were cultured in nutrient solution with different concentrations of glyphosate and the visual phytotoxicity on tea leaves was observed. The non-targeted analysis of non-volatile metabolites in the leaves and quantitative determination of shikimic acid and glyphosate in the leaves were carried out by ultra-high performance liquid chromatography-quadrupole orbitrap high-resolution mass spectrometry. The results show that the tea seedlings under the high dose of glyphosate (200 mg·L^-1) treatment exhibited characteristics of pesticide damage, while the tea seedlings under the low dose of glyphosate (50 mg·L^-1) treatment and control did not show apparent pesticide damage. Mass spectrometric and statistical analysis indicates that there were significant changes in the contents of shikimic acid pathway metabolites in the leaves of glyphosate-treated tea seedlings, with shikimic acid being one of the main differential metabolites. Within 21 d, the accumulation of shikimic acid in leaves was highly positively correlated with the absorption amount and action time of glyphosate. When the absorption amount of glyphosate was larger than 28 mg·kg^-1, the shikimic acid metabolism in tea plants was significantly inhibited, resulting in a large accumulation of shikimic acid in tea leaves. Compared with the control group, the content of shikimic acid in tea leaves affected by pesticides increased about 16-fold. This study shows that shikimic acid is one of the main metabolites of tea plants in response to glyphosate stress.

Key words: tea plant, glyphosate, shikimic acid, phytotoxicity, LC-MS

CLC Number:

S571.1
S482

LIU Hongxia, LIU Yingying, CHEN Hongping, CHAI Yunfeng. Glyphosate-stress Effects on Shikimic Acid in Tea Leaves[J]. Journal of Tea Science, 2023, 43(5): 657-666.

References

[1] Duke S O, Powles S B.Glyphosate: a once-in-a-century herbicide[J]. Pest Management Science, 2008, 64(4): 319-325.
[2] Acquavella J F, Alexander B H, Mandel J S, et al.Glyphosate biomonitoring for farmers and their families: results from the farm family exposure study[J]. Environmental Health Perspectives, 2004, 112(3): 321-326.
[3] 陶波, 蒋凌雪, 沈晓峰, 等. 草甘膦对土壤微生物的影响[J]. 中国油料作物学报, 2011, 33(2): 162-168, 179.
Tao B, Jiang L X, Shen X F, et al.Effects of glyphosate on soil microorganisms[J]. Chinese Journal of Oil Crop Sciences, 2011, 33(2): 162-168, 179.
[4] Aparicio V C, De Gerónimo E, Marino D, et al.Environmental fate of glyphosate and aminomethylphosphonic acid in surface waters and soil of agricultural basins[J]. Chemosphere, 2013, 93(9): 1866-1873.
[5] 郭永春, 陈金发, 赵峰, 等. 草甘膦及其代谢物氨甲基膦酸在茶树体中的分布研究[J]. 茶叶科学, 2020, 40(4): 510-518.
Guo Y C, Chen J F, Zhao F, et al.Study on the distribution of glyphosate and its metabolite aminomethylphosphonic acid in Camellia sinensis[J]. Journal of Tea Science, 2020, 40(4): 510-518.
[6] Servaites J C, Tucci M A, Geiger D R.Glyphosate effects on carbon assimilation, ribulose bisphosphate carboxylase activity, and metabolite levels in sugar beet leaves[J]. Plant Physiology, 1987, 85(2): 370-374.
[7] Zobiole L H S, Kremer R J, De Oliveira Jr. R S, et al. Glyphosate effects on photosynthesis, nutrient accumulation, and nodulation in glyphosate-resistant soybean[J]. Journal of Plant Nutrition and Soil Science, 2012, 175(2): 319-330.
[8] Reddy K N, Bellaloui N, Zablotowicz R M.Glyphosate effect on shikimate, nitrate reductase activity, yield, and seed composition in corn[J]. Journal of Agricultural and Food Chemistry, 2010, 58(6): 3646-3650.
[9] Helander M, Pauna A, Saikkonen K, et al.Glyphosate residues in soil affect crop plant germination and growth[J]. Scientific Reports, 2019, 9: 19653. doi: 10.1038/s41598-019-56195-3.
[10] Malalgoda M, Ohm J B, Howatt K A, et al.Effects of pre-harvest glyphosate use on protein composition and shikimic acid accumulation in spring wheat[J]. Food Chemistry, 2020, 332: 127422. doi: 10.1016/j.foodchem.2020.127422.
[11] Anderson K A, Cobb W T, Loper B R.Analytical method for determination of shikimic acid: Shikimic acid proportional to glyphosate application rates[J]. Communications in Soil Science and Plant Analysis, 2001, 32(17/18): 2831-2840.
[12] 高万君, 张永志, 童蒙蒙, 等. 茶园常用除草剂田间药效试验与残留动态[J]. 茶叶科学, 2019, 39(5): 587-594.
Gao W J, Zhang Y Z, Tong M M, et al.Weeds control effect and residues of several herbicides in tea gardens[J]. Journal of Tea Science, 2019, 39(5): 587-594.
[13] Tong M M, Gao W J, Jiao W T, et al.Uptake, translocation, metabolism, and distribution of glyphosate in nontarget tea plant (Camellia sinensis L.)[J]. Journal of Agricultural and Food Chemistry, 2017, 65(35): 7638-7646.
[14] 郭永春, 王淑燕, 王鹏杰, 等. 草甘膦对茶树叶片主要生化成分的影响[J]. 天然产物研究与开发, 2021, 33(3): 394-401, 499.
Guo Y C, Wang S Y, Wang P J, et al.Effect of glyphosate on main biochemical components of tea leaves[J]. Natural Product Research and Development, 2021, 33(3): 394-401, 499.
[15] 郭永春, 王鹏杰, 金珊, 等. 基于WGCNA鉴定茶树响应草甘膦相关的基因共表达模块[J]. 中国农业科学, 2022, 55(1): 152-166.
Guo Y C, Wang P J, Jin S, et al.Identification of co-expression gene related to tea plant response to glyphosate based on WGCNA[J]. Scientia Agricultura Sinica, 2022, 55(1): 152-166.
[16] Holländer-Czytko H, Amrhein N.Subcellular compartment of shikimic acid and phenylalanine in buckwheat cell suspension cultures grown in the presence of shikimate pathway inhibitors[J]. Plant Science Letters, 1983, 29(1): 89-96.
[17] Pline W A, Wilcut J W, Duke S O, et al.Tolerance and accumulation of shikimic acid in response to glyphosate applications in glyphosate-resistant and nonglyphosate-resistant cotton (Gossypium hirsutum L.)[J]. Journal of Agricultural and Food Chemistry, 2002, 50(3): 506-512.
[18] 诸力, 陈红平, 周苏娟, 等. 超高效液相色谱-串联质谱法测定不同茶叶中草甘膦、氨甲基膦酸及草铵膦的残留[J]. 分析化学, 2015, 43(2): 271-276.
Zhu L, Chen H P, Zhou S J, et al.Determination of glyphosate, aminomethyl phosphonic acid and glufosinate in different teas by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2015, 43(2): 271-276.
[19] Nilsson G.Effects of glyphosate on the amino acid content in spring wheat plants[J]. Swedish Journal of Agricultural Research, 1977, 7(3): 153-157.
[20] Wang C Y.Effect of glyphosate on aromatic amino acid metabolism in purple nutsedge (Cyperus rotundus)[J]. Weed Technology, 2001, 15(4): 628-635.
[21] 罗威, 高冬丽. 莽草酸含量测定方法综述[J]. 现代食品, 2020(10): 59-63.
Luo W, Gao D L.A review of the quantitative methods of shikimic acid content[J]. Modern Food, 2020(10): 59-63.
[22] Gachumi G, Purves R W, Hopf C, et al.Fast quantification without conventional chromatography, the growing power of mass spectrometry[J]. Analytical Chemistry, 2020, 92(13): 8628-8637.
[23] Campmajó G, Saurina J, Núñez O.FIA-HRMS fingerprinting subjected to chemometrics as a valuable tool to address food classification and authentication: application to red wine, paprika, and vegetable oil samples[J]. Food Chemistry, 2022, 373: 131491. doi: 10.1016/j.foodchem. 2021.
131491.
[24] Harring T, Streibig J C, Husted S.Accumulation of shikimic acid: a technique for screening glyphosate efficacy[J]. Journal of Agricultural and Food Chemistry, 1998, 46(10): 4406-4412.
[25] Schrübbers L C, Valverde B E, Sørensen J C, et al.Glyphosate spray drift in Coffea arabica-Sensitivity of coffee plants and possible use of shikimic acid as a biomarker for glyphosate exposure[J]. Pesticide Biochemistry and Physiology, 2014, 115: 15-22.
[26] 巩元勇, 郭书巧, 束红梅, 等. 1株抗草甘膦棉花突变体草甘膦抗性的初步鉴定[J]. 棉花学报, 2014, 26(1): 18-24.
Gong Y Y, Guo S Q, Shu H M, et al.Preliminary identification of glyphosate resistance of a new cotton mutant[J]. Cotton Science, 2014, 26(1): 18-24.

[1]	. [J]. Journal of Tea Science, 1965, 2(04): 59 -63 .
[2]	. [J]. Journal of Tea Science, 1965, 2(03): 18 -21 .
[3]	. [J]. Journal of Tea Science, 1965, 2(03): 22 -24 .
[4]	. [J]. Journal of Tea Science, 1966, 3(02): 55 -57 .
[5]	. [J]. Journal of Tea Science, 1966, 3(02): 82 -83 .
[6]	. [J]. Journal of Tea Science, 1986, 6(01): 1 -8 .
[7]	. [J]. Journal of Tea Science, 1987, 7(02): 29 -34 .
[8]	. [J]. Journal of Tea Science, 1988, 8(01): 37 -42 .
[9]	. [J]. Journal of Tea Science, 1995, 15(02): 154 .
[10]	. [J]. Journal of Tea Science, 1999, 19(01): 73 -76 .

Glyphosate-stress Effects on Shikimic Acid in Tea Leaves

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Liubin, WU Liyun, WEI Kang, WANG Liyuan. QTL Mapping and Candidate Gene Analysis for Timing of Spring Bud Flush in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2023, 43(6): 747-756.
[2]	LIU Dongna, GONG Xuejiao, LI Lanying, HUANG Fan, YAO Yu, XU Yaqiong, GAO Yuan, LUO Fan. Analysis of Photosynthetic and Fluorescence Characteristics of Albino Tea Plants [J]. Journal of Tea Science, 2023, 43(6): 757-768.
[3]	YANG Jun, ZHANG Lilan, ZHANG Wenjing, CHEN Linhai, ZHENG Guohua, LI Yijing, WANG Rangjian. Population Structure and Genetic Differences of Tea Germplasm Resources in Fujian [J]. Journal of Tea Science, 2023, 43(6): 769-783.
[4]	LI Yanchun, WANG Yixiang, YE Jing, LI Zhaowei. Changes of Rhizospheric Pathogen Alternaria sp. and Its Antagonistic Bacteria Pseudomonas sp. of Continuous Cropping Tea Plants Mediated by Phenolic Acids [J]. Journal of Tea Science, 2023, 43(6): 823-834.
[5]	YI Huajuan, ZHU Jieling, ZHOU Ruizheng, ZHENG Yaolin, ZHANG Shuquan, YANG Le, SU Yonglun. Determination of Glyphosate and Its Metabolites in Tea by Automatic Solid Phase Extraction Combined with UPLC-MS/MS [J]. Journal of Tea Science, 2023, 43(6): 857-869.
[6]	YANG Jihong, ZHOU Hanchen, XU Yujie. Catalytic Function, Promoter Structure and Functional Analysis of CsNUDX1-cyto in Different Tea Cultivars [J]. Journal of Tea Science, 2023, 43(5): 621-630.
[7]	TANG Ziyi, DU Yue, YANG Hongbin, LI Xinghui, YU Youben, WANG Weidong. Changes of Endogenous Hormone Contents and Expression Analysis of Related Genes in Leaves of Tea Plants Under Heat and Drought Stresses [J]. Journal of Tea Science, 2023, 43(4): 489-500.
[8]	HAN Haidong, ZHOU Liuting, HUANG Xiaoyun, YU Chengran, HUANG Xiusheng. The Characteristics of Fungal Community Structure in Tea Rhizosphere Soil Interplanted with Ganoderma lucidum Based on High-throughput Sequencing Technology [J]. Journal of Tea Science, 2023, 43(4): 513-524.
[9]	SUN Yue, LIU Mengyue, GAO Chenxi, WU Quanjin, CAO Shixian, YU Shuntian, CHEN Zhidan, JIN Shan, SUN Weijiang. Study on the Differences of Leaf Color and Volatiles of Different Insect-resistance Tea Cultivars [J]. Journal of Tea Science, 2023, 43(4): 525-543.
[10]	LI Jiasi, LIU Yingqing, ZHANG Yongheng, ZHANG Ying'ao, XIAO Yezi, LIU Lu, YU Youben. Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress [J]. Journal of Tea Science, 2023, 43(3): 325-334.
[11]	SHEN Ruihan, MA Lifeng, YANG Xiangde, FANG Li. Effects of Nitrogen Form and Weak Light Stress on Tea Plant Growth and Metabolism [J]. Journal of Tea Science, 2023, 43(3): 349-355.
[12]	GUO Lina, HAO Xinyuan, WANG Lu, QI Meng, LI Xiaoman, REN Hengze, ZHENG Qinghua, WANG Xinchao, ZENG Jianming. Study on the Characteristics of CsPHT1;3 and Its Response to Selenium in Tea Plants [J]. Journal of Tea Science, 2023, 43(2): 173-182.
[13]	LI Hongli, ZHOU Tiefeng, MAO Yuxiao, HUANG Haitao, CUI Hongchun, ZHENG Xuxia, ZHAO Yun. Isolation and Identification of Anthracnose Pathogen from Xihu Longjing Plantation and Screening of Its Plant-derived Fungicides [J]. Journal of Tea Science, 2023, 43(2): 194-204.
[14]	ZHENG Shizhong, ZHOU Ziwei, CHEN Xiaohui, CAI Liewei, JIANG Shengtao, LIU Shengrong. Screening, Identification and Culture Condition Optimization of Antagonistic Endophytic Bacteria Against Gloeosporium theae-sinensis Miyake [J]. Journal of Tea Science, 2023, 43(2): 205-215.
[15]	QIU Shiting, HOU Xue, LEI Shaorong, HAN Mei, HE Guangyun, LI Ying, QIN Shudi. Simultaneous Determination of Nicotinamide Adenine Dinucleotide and Its Four Precursors in Tea by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. Journal of Tea Science, 2023, 43(2): 216-226.