福建茶树种质资源群体结构及遗传差异

doi:10.13305/j.cnki.jts.2023.06.006

Abstract

Abstract: The population genetic structure, genetic diversity, genetic differentiation, gene flow, molecular variance of 208 tea germplasm resources in Fujian were studied by using 38 pairs of SSR fluorescent primers, and the leaf traits were also investigated. The results show that the Nei’s genetic diversity index and Shannon’s information index of 208 tea resources in Fujian were 0.674 and 1.444, respectively. The average values of leaf area and length-width ratio were 27.442 cm² and 2.516, respectively. The genetic variation of tea germplasm in Fujian were mainly originated from individual genetic variation. The test materials were divided into 8 groups by the structure software analysis. The material sources in groups a, b, f, and h were single, while the material sources in groups c, d, e, and g were complex. The genetic background of tea population in different regions was similar. There were a total of 40 tea cultivars from Fujian in groups a, b, and e. Group a was mainly cultivars suitable for processing green tea. Group b was mainly cultivars suitable for processing oolong tea. The representative cultivars within group e were suitable for processing green tea. There is a certain correlation between the group attributes of group a, group b, group e and the suitable tea cultivars. Group c included the resources from Nanjing county, Yunxiao county and Pinghe county, with relatively close geographical locations. The group attributes are related to geographical sources. The gene flow value between group g and group e was 6.321, indicating frequent gene exchange between groups. The clustering of group similarity coefficients shows that group d and group b were closely related. The genetic differentiation between group h and other groups was obvious, and the characters of leaf area and leaf serration number were significantly different (P<0.05). The genetic relationship between group f and other groups was relatively distant, and there were significant differences in the characteristics of leaf serration number and leaf vein logarithm (P<0.05), showing that both group h and group f had certain uniqueness and need further identification. These research results provided a certain reference for the identification, screening, and utilization of tea germplasm resources in Fujian.

Key words: tea plant, Fujian, population structure, genetic differentiation

CLC Number:

S571.1
S324

YANG Jun, ZHANG Lilan, ZHANG Wenjing, CHEN Linhai, ZHENG Guohua, LI Yijing, WANG Rangjian. Population Structure and Genetic Differences of Tea Germplasm Resources in Fujian[J]. Journal of Tea Science, 2023, 43(6): 769-783.

References 39

[1]	马建强, 姚明哲, 陈亮. 茶树种质资源研究进展[J]. 茶叶科学, 2015, 35(1): 11-16.Ma J Q, Yao M Z, Chen L.Research progress on germplasms of tea plant (Camellia sinensis)[J]. Journal of Tea Science, 2015, 35(1): 11-16.
[2]	王丽鸳, 姜燕华, 段云裳, 等. 基于SSR分子标记的龙井群体种的遗传多样性及遗传分化研究[J]. 茶叶科学, 2011, 31(1): 40-44.Wang L Y, Jiang Y H, Duan Y S, et al.Genetic diversity and differentiation of Longjing tea population based on SSR markers[J]. Journal of Tea Science, 2011, 31(1): 40-44.
[3]	雷雨, 段继华, 李赛君, 等. 茶树新品系“53-34”父本的EST-SSR标记鉴定[J]. 茶叶通讯, 2016, 43(1): 28-31.Lei Y, Duan J H, Li S J, et al.Identification of male parents for “53-34” tea cultivar based on SSR markers[J]. Journal of Tea Communication, 2016, 43(1): 28-31.
[4]	陈熙, 李佼, 张羽, 等. 基于EST-SSR的陕西茶树资源遗传多样性分析[J]. 四川农业大学学报, 2016, 34(3): 322-327.Chen X, Li J, Zhang Y, et al.Genetic diversity analysis of tea in Shaanxi province based on EST-SSR[J]. Journal of Sichuan Agricultural University, 2016, 34(3): 322-327.
[5]	陈勋, 龚自明. 基于EST-SSR标记的湖北茶树种质资源遗传多样性分析[J]. 分子植物育种, 2017, 15(5): 1831-1838.Chen X, Gong Z M.Analysis of genetic diversity with EST-SSR markers for tea germplasm in Hubei province[J]. Molecular Plant Breeding, 2017, 15(5): 1831-1838.
[6]	刘冠群, 谭礼强, 吴祠平, 等. 基于SSR标记的四川移栽野生大茶树的遗传多样性[J]. 贵州农业科学, 2018, 46(4): 4-8.Liu G Q, Tan L Q, Wu C P, et al.Genetic diversity of Sichuan transplanted wild tea tree by SSR marker[J]. Guizhou Agricultural Sciences, 2018, 46(4): 4-8.
[7]	尚卫琼, 段志芬, 杨毅坚, 等. 基于EST-SSR标记的云南大叶茶资源遗传多样性分析[J]. 山东农业科学, 2018, 50(1): 16-22.Shang W Q, Duan Z F, Yang Y J, et al.Genetic diversity analysis of Daye tea germplasm in Yunnan based on EST-SSR markers[J]. Shandong Agricultural Sciences, 2018, 50(1): 16-22.
[8]	罗亦纾, 张霞, 毛君林, 等. 35份重庆大茶树种质资源遗传多样性的SSR分析[J]. 分子植物育种, 2019, 17(19): 6549-6557.Luo Y S, Zhang X, Mao J L, et al.Analysis of genetic diversity in 35 ancient tea accessions from Chongqing with SSR markers[J]. Molecular Plant Breeding, 2019, 17(19): 6549-6557.
[9]	彭靖茹, 李朝昌, 檀业维, 等. 基于SSR分子标记的广西德保县和隆林县野生古茶树聚类分析[J]. 南方农业学报, 2019, 50(1): 1-7.Peng J R, Li C C, Tan Y W, et al.Clustering analysis for wild ancient tea germplasm resources in Debao county and Longlin county, Guangxi based on SSR molecular markers[J]. Journal of Southern Agriculture, 2019, 50(1): 1-7.
[10]	安红卫, 宋勤飞, 牛素贞. 贵州茶树种质资源遗传多样性、群体结构和遗传分化研究[J]. 浙江农业学报, 2021, 33(7): 1234-1243.An H W, Song Q F, Niu S Z.Study on genetic diversity, population structure and genetic differentiation of tea germplasm in Guizhou[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1234-1243.
[11]	魏世平, 刘晓芬, 杨胜先, 等. 中国栽培大豆群体结构不同分类方法的比较[J]. 南京农业大学学报, 2011, 34(2): 13-17.Wei S P, Liu X F, Yang S X, et al.Comparison of various clustering methods for population structure in Chinese cultivated soybean (Glycine max (L.) Merr.)[J]. Journal of Nanjing Agricultural University, 2011, 34(2): 13-17.
[12]	Yao M Z, Ma C L, Chen L, et al.Diversity distribution and population structure of tea germplasms in China revealed by EST-SSR markers[J]. Tree Genetics & Genomes, 2012, 8: 205-220.
[13]	袁王俊, 叶松, 董美芳, 等. 菊花品种表型性状与SSR和SCoT分子标记的关联分析[J]. 园艺学报, 2017, 44(2): 364-372.Yuan W J, Ye S, Dong M F, et al.Association analysis of phenotypic traits with SSR and SCoT molecular markers in Chrysanthemum morifolium[J]. Acta Horticulturae Sinica, 2017, 44(2): 364-372.
[14]	张晨, 云岚, 李珍, 等. 新麦草种质的SSR遗传多样性及群体结构分析[J]. 植物遗传资源学报, 2021, 20(1): 48-59.Zhang C, Yun L, Li Z, et al.Genetic diversity and structure analysis in Psathyrostachys Nevski population using SSR markers[J]. Journal of Plant Genetic Resources, 2021, 20(1): 48-59.
[15]	王亮, 韦继光, 葛春峰, 等. 基于SSR标记分析蓝莓品种‘蓝美1号’自由授粉子代遗传多样性及群体遗传结构[J]. 植物资源与环境学报, 2022, 31(3): 35-43.Wang L, Wei J G, Ge C F, et al.Analysis on genetic diversity and population genetic structure of open-pollinated progenies of Vaccinium corymbosum ‘Lanmei 1’ based on SSR marker[J]. Journal of Plant Resources and Environment, 2022, 31(3): 35-43.
[16]	苏一钧, 王娇, 戴习彬, 等. 303份甘薯地方种SSR遗传多样性与群体结构分析[J]. 植物遗传资源学报, 2018, 19(2): 243-251.Su Y J, Wang J, Dai X B, et al.Genetic diversity and population structure analysis of 303 sweetpotato landraces using SSR markers[J]. Journal of Plant Genetic Resources, 2018, 19(2): 243-251.
[17]	金基强, 崔海瑞, 龚晓春, 等. 用EST-SSR标记对茶树种质资源的研究[J]. 遗传, 2007, 29(1): 103-108.Jin J Q, Cui H R, Gong X C, et al.Studies on tea plants (Camellia sinensis) germplasms using EST-SSR marker[J]. Hereditas, 2007, 29(1): 103-108.
[18]	Ma J Q, Yao M Z, Chen L, et al.Construction of a SSR-based genetic map and identification of QTLs for catechins content in tea plant (Camellia sinensis)[J]. Plos One, 2014, 9(3): e93131. doi: 10.1371/journal.pone.0093131.
[19]	陈亮, 杨亚军, 虞富莲, 等. 茶树种质资源描述规范和数据标准[M]. 北京: 中国农业出版社, 2005.Cheng L, Yang Y J, Yu F L, et al.Description and data standard of tea (Camellia spp.) [M]. Beijing: China Agriculture Press, 2005.
[20]	Pritchard J K, Stephens M, Donnelly P.Inference of population structure using multilocus genotype data[J]. Genetics, 2000, 155: 945-959.
[21]	刘志斋, 吴迅, 刘海利, 等. 基于40个核心SSR标记揭示的820份中国玉米重要自交系的遗传多样性与群体结构[J]. 中国农业科学, 2012, 45(11): 2107-2138.Liu Z Z, Wu X, Liu H L, et al.Genetic diversity and population structure of important Chinese maize inbred lines revealed by 40 core simple sequence repeats (SSRs)[J]. Scientia Agricultura Sinica, 2012, 45(11): 2107-2138.
[22]	崔蕾, 谢从新, 李艳和, 等. 斑点叉尾鮰4个群体遗传多样性的微卫星分析[J]. 华中农业大学学报, 2012, 31(6): 744-751.Cui L, Xie C X, Li Y H, et al.Analysis of genetic diversity among four different channel catfish populations by using microsatellite markers[J]. Journal of Huazhong Agricultural University, 2012, 31(6): 744-751.
[23]	Sved J A.The stability of linked systems of loci with a small population size[J], Genetics, 1968, 59(4): 543-563.
[24]	杨阳, 刘振, 赵洋, 等. 利用EST-SSR标记研究黄金茶群体遗传多样性及遗传分化[J]. 茶叶科学, 2009, 29(3): 236-242.Yang Y, Liu Z, Zhao Y, et al.Genetic diversity and relationship of Huangjincha cultivar based on EST-SSR markers[J]. Journal of Tea Science, 2009, 29(3): 236-242.
[25]	黄晓霞, 唐探, 姜永雷, 等. 千家寨不同海拔野生茶树的EST-SSR遗传多样性研究[J]. 茶叶科学, 2019, 35(4): 347-353.Huang X X, Tang T, Jiang Y L, et al.Genetic diversity of wild tea plant in different altitude in Qianjiazhai[J]. Journal of Tea Science, 2019, 35(4): 347-353.
[26]	张明泽, 姚玉仙, 陈世军. 黔南60份茶树种质资源遗传多样性的SSR分析[J]. 西北植物学报, 2016, 36(6): 1117-1124.Zhang M Z, Yao Y X, Chen S J.Genetic diversity analysis of tea germplasm in Qiannan prefecture by SSR markers[J]. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(6): 1117-1124.
[27]	姜晓辉, 方开星, 陈栋, 等. 基于EST-SSR毛细管电泳荧光标记技术分析广东2个历史名茶群体遗传多样性[J]. 热带作物学报, 2018, 39(1): 46-54.Jiang X H, Fang K X, Chen D, et al.Genetic diversity and relationship of two historical famous Camellia sinensis groups in Guangdong by capillary electrophoresis detcection with fluorescent EST-SSR marker[J]. Chinese Journal of Tropical Crops, 2018, 39(1): 46-54.
[28]	黄寿辉, 温立香, 彭静茹, 等. 广西部分地区野生茶树遗传关系EST-SSR标记分析[J]. 广西植物, 2019, 39(6): 821-830.Huang S H, Wen L X, Peng J R, et al.Genetic relationship analysis of wild tea tree germplasm resources in part of Guangxi based on EST-SSR markers[J]. Guihaia, 2019, 39(6): 821-830.
[29]	谢文钢, 李晓松, 谭礼强, 等. 四川茶树资源遗传多样性及高EGCG资源筛选[J]. 热带作物学报, 2020, 41(12): 2430-2438.Xie W G, Li X S, Tan L Q, et al.Genetic diversity of tea resources and screening high EGCG germplasms in Sichuan province[J]. Chinese Journal of Tropical Crops, 2020, 41(12): 2430-2438.
[30]	刘升锐, 董心雨, 郭锐, 等. 安徽省“太平猴魁”茶区柿大茶群体种遗传多样性的SSR分析[J]. 安徽农业大学学报, 2020, 47(4): 499-504.Liu S R, Dong X Y, Guo R, et al.Genetic diversity of tea plant population in “Taipinghoukui” tea production area of Anhui province based on SSR markers[J]. Journal of Anhui Agricultural University, 2020, 47(4): 499-504.
[31]	尚卫琼, 李友勇, 刘悦, 等. 基于EST-SSR标记的西双版纳苦茶资源遗传多样性分析[J]. 山西农业科学, 2020, 48(2): 167-171.Shang W Q, Li Y Y, Liu Y, et al.Genetic diversity analysis of bitter tea germplasm resource in Xishuangbanna based on EST-SSR markers[J]. Journal of Shanxi Agricultural Sciences, 2020, 48(2): 167-171.
[32]	叶春秀, 李全胜, 李有忠, 等. 新疆早熟陆地棉SSR标记遗传多样性及群体结构分析[J]. 西南农业报, 2015, 28(3): 997-1002.Ye C X, Li Q S, Li Y Z, et al.Genetic diversity and population structure of earliness upland cotton cultivars in Xinjiang by SSR molecular marker[J]. Southwest China Journal of Agricultural Sciences, 2015, 28(3): 997-1002.
[33]	寇淑君, 霍阿红, 付国庆, 等. 利用荧光SSR分析中国糜子的遗传多样性和群体遗传结构[J]. 中国农业科学, 2019, 52(9): 1475-1487.Kou S J, Huo A H, Fu G Q, et al.Genetic diversity and population structure of broomcorn millet in China based on fluorescently labeled SSR[J]. Scientia Agricultura Sinica, 2019, 52(9): 1475-1487.
[34]	文自翔, 赵团结, 郑永战, 等. 中国栽培和野生大豆农艺品质性状与SSR标记的关联分析 I. 群体结构及关联标记[J]. 作物学报, 2008, 34(7): 1169-1178.Wen Z X, Zhao T J, Zheng Y Z, et al.Association analysis of agronomic and quality traits with SSR markers in Glycine max and Glycine soja in China: I. Population structure and associated markers[J]. Acta Agronomica Sinica, 2008, 34(7): 1169-1178.
[35]	卢俊浩, 张婷, 张鹏, 等. 基于SSR标记的花菜类作物遗传多样性及群体结构分析[J]. 西南南农业学报, 2022, 35(8): 1887-1894.Lu J H, Zhang T, Zhang P, et al.Analysis of genetic diversity and population structure of caulinower crops based on SSR markers[J]. Southwest China Journal of Agricultural Sciences, 2022, 35(8): 1887-1894.
[36]	赵旗峰, 黄丽萍, 王敏, 等. 基于SSR标记的100份葡萄资源亲缘关系和群体遗传结构分析[J]. 果树学报, 2021, 38(8): 1217-1230.Zhao Q F, Huang L P, Wang M, et al.Genetic relationship and population genetic structure analysis of 100 accessions of grape germplasm resources based on SSR markers[J]. Journal of Fruit Science, 2021, 38(8): 1217-1230.
[37]	吉琼, 张新玲, 童婷, 等. 利用SSR标记研究96个玉米自交系的遗传多样性及其群体遗传结构[J]. 新疆农业大学学报, 2012, 35(2): 99-106.Ji Q, Zhang X L, Tong T, et al.Studies on genetic diversity and population genetic structure of 96 maize inbred lines using SSR markers[J]. Journal of Xinjiang Agricultural University, 2012, 35(2): 99-106.
[38]	吴晓梅, 姚明哲, 陈亮, 等. 利用EST-SSR标记研究适制绿茶与乌龙茶品种的遗传多样性与遗传结构[J]. 茶叶科学, 2010, 30(3): 195-202.Wu X M, Yao M Z, Chen L, et al.Genetic diversity and population structure among green tea and oolong tea cultivars based on EST-SSR markers[J]. Journal of Tea Science, 2010, 30(3): 195-202.
[39]	陈常颂, 余文权, 王庆森, 等. 福建省茶树品种图志[M]. 北京: 中国农业科学技术出版社, 2016.Chen C S, Yu W Q, Wang Q S, et al.Atlas of tea plant varieties in Fujian province [M]. Beijing: China Agricultural Science and Technoloy Press, 2016.

Population Structure and Genetic Differences of Tea Germplasm Resources in Fujian

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 39

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Liubin, WU Liyun, WEI Kang, WANG Liyuan. QTL Mapping and Candidate Gene Analysis for Timing of Spring Bud Flush in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2023, 43(6): 747-756.
[2]	LIU Dongna, GONG Xuejiao, LI Lanying, HUANG Fan, YAO Yu, XU Yaqiong, GAO Yuan, LUO Fan. Analysis of Photosynthetic and Fluorescence Characteristics of Albino Tea Plants [J]. Journal of Tea Science, 2023, 43(6): 757-768.
[3]	LI Yanchun, WANG Yixiang, YE Jing, LI Zhaowei. Changes of Rhizospheric Pathogen Alternaria sp. and Its Antagonistic Bacteria Pseudomonas sp. of Continuous Cropping Tea Plants Mediated by Phenolic Acids [J]. Journal of Tea Science, 2023, 43(6): 823-834.
[4]	YANG Jihong, ZHOU Hanchen, XU Yujie. Catalytic Function, Promoter Structure and Functional Analysis of CsNUDX1-cyto in Different Tea Cultivars [J]. Journal of Tea Science, 2023, 43(5): 621-630.
[5]	LIU Hongxia, LIU Yingying, CHEN Hongping, CHAI Yunfeng. Glyphosate-stress Effects on Shikimic Acid in Tea Leaves [J]. Journal of Tea Science, 2023, 43(5): 657-666.
[6]	TANG Ziyi, DU Yue, YANG Hongbin, LI Xinghui, YU Youben, WANG Weidong. Changes of Endogenous Hormone Contents and Expression Analysis of Related Genes in Leaves of Tea Plants Under Heat and Drought Stresses [J]. Journal of Tea Science, 2023, 43(4): 489-500.
[7]	HAN Haidong, ZHOU Liuting, HUANG Xiaoyun, YU Chengran, HUANG Xiusheng. The Characteristics of Fungal Community Structure in Tea Rhizosphere Soil Interplanted with Ganoderma lucidum Based on High-throughput Sequencing Technology [J]. Journal of Tea Science, 2023, 43(4): 513-524.
[8]	SUN Yue, LIU Mengyue, GAO Chenxi, WU Quanjin, CAO Shixian, YU Shuntian, CHEN Zhidan, JIN Shan, SUN Weijiang. Study on the Differences of Leaf Color and Volatiles of Different Insect-resistance Tea Cultivars [J]. Journal of Tea Science, 2023, 43(4): 525-543.
[9]	LI Jiasi, LIU Yingqing, ZHANG Yongheng, ZHANG Ying'ao, XIAO Yezi, LIU Lu, YU Youben. Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress [J]. Journal of Tea Science, 2023, 43(3): 325-334.
[10]	SHEN Ruihan, MA Lifeng, YANG Xiangde, FANG Li. Effects of Nitrogen Form and Weak Light Stress on Tea Plant Growth and Metabolism [J]. Journal of Tea Science, 2023, 43(3): 349-355.
[11]	MENG Rongjun, CHEN Liang, XU Yuan, LIN Wei, ZHOU Qiwei, XIE Yilin, LAI Dingqing, LAI Jiaye. Genetic Diversity Analysis of Tea Genetic Resources in Sanjiang, Guangxi [J]. Journal of Tea Science, 2023, 43(2): 147-158.
[12]	GUO Lina, HAO Xinyuan, WANG Lu, QI Meng, LI Xiaoman, REN Hengze, ZHENG Qinghua, WANG Xinchao, ZENG Jianming. Study on the Characteristics of CsPHT1;3 and Its Response to Selenium in Tea Plants [J]. Journal of Tea Science, 2023, 43(2): 173-182.
[13]	LI Hongli, ZHOU Tiefeng, MAO Yuxiao, HUANG Haitao, CUI Hongchun, ZHENG Xuxia, ZHAO Yun. Isolation and Identification of Anthracnose Pathogen from Xihu Longjing Plantation and Screening of Its Plant-derived Fungicides [J]. Journal of Tea Science, 2023, 43(2): 194-204.
[14]	ZHENG Shizhong, ZHOU Ziwei, CHEN Xiaohui, CAI Liewei, JIANG Shengtao, LIU Shengrong. Screening, Identification and Culture Condition Optimization of Antagonistic Endophytic Bacteria Against Gloeosporium theae-sinensis Miyake [J]. Journal of Tea Science, 2023, 43(2): 205-215.
[15]	LIU Haoran, ZHANG Chenyu, GONG Yang, YE Yuanyuan, CHEN Jiedan, CHEN Liang, LIU Dingding, MA Chunlei. Development and Application of Albinotea Plant mSNP Molecular Markers Based on Genome-wide Resequencing [J]. Journal of Tea Science, 2023, 43(1): 27-39.