EGCG对高脂饮食GK大鼠白色脂肪米色化的诱导作用与机制研究

doi:10.13305/j.cnki.jts.2024.01.008

Abstract

Abstract: The types of adipose tissue are closely related to human metabolism. Transforming white adipocytes into thermogenic beige adipocytes through dietary or nutritional interventions is a safe strategy to reduce fat accumulation and regulate metabolism. Currently, research on the role of white adipose tissue beiging has mainly focused on obese populations. To explore the effect of EGCG on promoting the beiging of white adipose tissue in non-obese individuals with metabolic disorders and its related mechanisms, this study used non-obese, spontaneously diabetic type 2 GK rats. These rats were fed a high-fat diet and received 40 mg·kg^-1 and 80 mg·kg^-1 EGCG daily by gavage. In this study, we assessed body weight, food intake, cellular morphology of adipose tissue, gene expression levels associated with beiging, and protein expression levels of UCP1 in GK rats. Additionally, transcriptome sequencing was also performed on epididymal white adipose tissue. The results show that gavage intervention with 80 mg·kg^-1 EGCG has no significant effect on the food intake and body weight of GK rats. It induced a trend of beiging in adipocytes towards a multilocular phenotype transformation, characterized by a decrease in cell size and an increase in cell number. Moreover, it significantly upregulated the expression levels of beiging-related genes Pparg, Ppargc1a, Ucp1 and the protein expression level of UCP1.This demonstrates the inducing effect of EGCG on the beiging of visceral epididymal white adipose tissue in high-fat diet-fed GK rats, indicating its potential in the regulation of lipid metabolism. Combined with transcriptome analysis, the results suggest that the induction mechanism of EGCG on the beiging of white adipose tissue in high-fat diet-fed GK rats may be associated with the PPAR signaling pathway, PI3K/Akt, and MAPK signaling pathway.

Key words: EGCG, non-obesetype, Goto-Kakizaki rats, white adipose tissue, beiging

CLC Number:

WAN Liwei, ZENG Hongzhe, PENG Liyuan, WEN Shuai, LIU Changwei, BAO Sudu, AN Qin, HUANG Jian'an, LIU Zhonghua. Inductive Effect and Mechanism of EGCG on Beiging of White Adipose Tissue in High-fat Diet-fed GK Rats[J]. Journal of Tea Science, 2024, 44(1): 119-132.

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Inductive Effect and Mechanism of EGCG on Beiging of White Adipose Tissue in High-fat Diet-fed GK Rats

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