茶树STOP基因家族的鉴定及表达模式分析

doi:10.13305/j.cnki.jts.2024.03.001

Abstract

Abstract: STOP (Sensitive to proton rhizotoxicity) is a type of C₂H₂ zinc finger transcription factor, and it plays an important regulatory role in various stress tolerance mechanisms in higher plants. A total of 6 STOP genes were identified based on the whole genome data of tea plant (Camellia sinensis), and analyzed by bioinformatics and real-time fluorescence quantitative PCR. The results show that the six CsSTOP genes encoded 376-505 amino acids, their molecular weights were 42.17-56.36 kDa, and their theoretical isoelectric points were 5.53-8.85, all of which were unstable proteins. Conserved domain analysis of the proteins shows that they all contained zf-C₂H₂ conserved domain. Phylogenetic analysis shows that tea plant has high homology with Arabidopsis, Citrus sinensis and Nicotiana tabacum. Cis-acting element analysis of the promoter shows that CsSTOPs contain many elements related to growth and development, hormone response and abiotic stress. Transcriptome data analysis of different tissues shows that the expression level of CsSTOP1 was the higher in roots, fruits and mature leaves, the expression level of CsSTOP2 was the higher in young leaves, the expression level of CsSTOP3 was the higher in old leaves, and the expression levels of CsSTOP4 and CsSTOP5 were low in all tissues. The expressions of different CsSTOP genes were induced by PEG-induced drought stress, salt stress, methyl jasmonate stress and cold stress, indicating that CsSTOP genes were involved in the regulation of growth and development of tea plants and response to abiotic stress. Fluorescence quantitative PCR detection shows that the expression levels of CsSTOPs, CsGS1s and CsGDHs in leaves and roots of 'Emeiwenchun' treated with high NH₄⁺ concentration (4.5 mmol·L^-1) were higher than those in the control treatment (CK). Particularly, the expression levels of CsSTOPs, CsGS1.1, CsGS1.3 and CsGDH2 were significantly higher than CK in leaves treated with high NH₄⁺ concentration. In this study, the basic characteristics and functions of CsSTOPs were preliminarily analyzed, and it was found that CsSTOPs could coordinate with CsGS1s and CsGDHs genes to regulate the process of tea plant adaptation to high NH₄⁺ environmental availability.

Key words: tea plant, STOP gene family, bioinformatics analysis, gene expression

CLC Number:

S571.1
Q52

LONG Lu, TANG Dandan, CHEN Wei, TAN Liqiang, CHEN Shengxiang, TANG Qian. Identification and Expression Pattern Analysis of STOP Gene Family in Tea Plants (Camellia sinensis)[J]. Journal of Tea Science, 2024, 44(3): 386-398.

References

[1] 马哲宇, 高可可, 李桂新, 等. STOP1介导多种逆境响应分子机制的研究进展[J]. 植物生理学报, 2023, 59(4): 773-781.
Ma Z Y, Gao K K, Li G X, et al.Research progress of the molecular mechanisms of STOP1 in various stress responses[J]. Plant Physiology Journal, 2023, 59(4): 773-781.
[2] Iuchi S, Koyama H, Iuchi A, et al.Zinc finger protein STOP1 is critical for proton tolerance in Arabidopsis and coregulates a key gene in aluminum tolerance[J]. Proceedings of the National Academy of Sciences, 2007, 104(23): 9900-9905.
[3] Yamaji N, Huang C F, Nagao S, et al.A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice[J]. Plant Cell, 2009, 21(10): 3339-3349.
[4] Yoshinao O, Hiroki I, Yuriko K, et al.Characterization of AtSTOP1 orthologous genes in tobacco and other plant species[J]. Plant Physiology, 2013, 162(4): 1937-1946.
[5] Luísa G A, César B, Pilar P, et al.Molecular characterization of TaSTOP1 homoeologues and their response to aluminium and proton (H+) toxicity in bread wheat (Triticum aestivum L.)[J]. BMC Plant Biology, 2013, 13(1): 134. doi: 10.1186/1471-2229-13-134.
[6] 丛亚辉, 王婷婷, 柳聚阁, 等. 大豆耐铝毒候选基因GmSTOP1的克隆与表达分析[J]. 作物学报, 2015, 41(12):1802-1809.
Cong Y H, Wang T T, Liu J G, et al.Cloning and expression analysis of tolerance to aluminum-toxicity candidate gene GmSTOP1 in soybean[J]. Acta Agronomica Sinica, 2015, 41(12): 1802-1809.
[7] 张宝云. 紫花苜蓿铝胁迫响应基因MsMATE与MsSTOP1的功能与表达调控研究[D]. 重庆: 重庆大学, 2017.
Zang B Y.Function and expression regulation of aluminum-responsive genes MsMATE and MsSTOP1 in Medicago sativa [D]. Chongqing: Chongqing University, 2017.
[8] 罗佳佳, 向晨莹, 刘攀道, 等. 柱花草SgSTOP1和SgSTOP2基因的克隆与表达分析[J]. 草业科学, 2019, 36(3): 704-712.
Luo J J, Xiang C Y, Liu P D, et al.Cloning and expression analysis of SgSTOP1 and SgSTOP2 in Stylosanthes guianensis[J]. Pratacultural Science, 2019, 36(3): 704-712.
[9] 张永福, 徐仕琴, 陈姣, 等. 葡萄耐铝毒基因STOP1的克隆与表达分析[J]. 西南农业学报, 2022, 35(3): 588-595.
Zhang Y F, Xu S Q, Chen J, et al.Cloning and expression analysis of tolerance to aluminum-toxicity gene STOP1 in Vitis[J]. Southwest China Journal of Agricultural Sciences, 2022, 35(3): 588-595.
[10] Kobayashi Y, Ohyama Y, Kobayashi Y, et al.STOP2 activates transcription of several genes for Al- and low pH-tolerance that are regulated by STOP1 in Arabidopsis[J]. Molecular Plant, 2014, 7(2): 311-322.
[11] Sadhukhan A, Enomoto T, Kobayashi Y, et al.Sensitive to proton rhizotoxicity1 regulates salt and drought tolerance of Arabidopsis thaliana through transcriptional regulation of CIPK23[J]. Plant and Cell Physiology, 2019, 60(9): 2113-2126.
[12] Enomoto T, Tokizawa M, Ito H, et al. STOP1 regulates the expression of HsfA2 and GDHs that are critical for low-oxygen tolerance in Arabidopsis[J]. Journal of Experimental Botany, 2019, 70(12): 3297-3311.
[13] 田文昊. 拟南芥STOP1蛋白协同氮磷营养的分子机制研究[D]. 杭州: 浙江大学, 2021.
Tian W H.Mechanisms of nitrogen and phosphorus acquisition coordinated by STOP1 in Arabidopsis [D]. Hangzhou: Zhejiang University, 2021.
[14] 方翔, 胡国策, 孙琪璐, 等. 氮素形态对茶树叶片品质及其氮代谢相关基因的影响[J]. 西北农林科技大学学报(自然科学版), 2020, 48(2): 52-59.
Fang X, Hu G C, Sun Q L, et al.Effects of nitrogen forms on tea quality and nitrogen metabolism related genes in tea leaves[J]. Journal of Northwest A & F University (Natural Science Edition), 2020, 48(2): 52-59.
[15] Britto D T, Kronzucker H J.NH₄+ toxicity in higher plants: a critical review[J]. Journal of Plant Physiology, 2002, 159(6): 567-584.
[16] Bittsánszky A, Pilinszky K, Gyulai G, et al.Overcoming ammonium toxicity[J]. Plant Science, 2015, 231(1): 184-190.
[17] 范子晗. 柑橘铵毒害产生机制及缓解措施研究[D]. 重庆: 西南大学, 2021.
Fan Z H.Study on the mechanism and relative alleviation measures of ammonium toxicity to citrus [D]. Chongqing: Southwest University, 2021.
[18] Tang D D, Liu M Y, Zhang Q F, et al.Preferential assimilation of NH₄+ over NO₃- in tea plant associated with genes involved in nitrogen transportation, utilization and catechins biosynthesis[J]. Plant Science, 2020, 29: 110369. doi: 10.1016/j.plantsci.2019.110369.
[19] Wang Y, Wang Y M, Lu Y T, et al.Influence of different nitrogen sources on carbon and nitrogen metabolism and gene expression in tea plants (Camellia sinensis L.)[J]. Plant Physiology and Biochemistry: PPB, 2021, 167(1): 561-566.
[20] 胡国策, 蒋家月, 田坤红, 等. 氮素形态和水平对茶树生理特性的影响[J]. 安徽农业大学学报, 2018, 45(4): 588-593.
Hu G C, Jang J Y, Tian K H, et al.Effects of nitrogen forms and nitrogen levels on the physiological characteristics of tea plants[J]. Journal of Anhui Agricultural University, 2018, 45(4): 588-593.
[21] 李勇. 茶树响应铝的遗传变异及铝富集候选基因挖掘[D]. 武汉: 华中农业大学, 2017.
Li Y.The genetic variation of tea plant [Camellia sinensis (L.) O. Ktze.] in response to aluminum and candidate genes related to its Al accumulation [D]. Wuhan: Huazhong Agricultural University, 2017.
[22] 李营营. 茶树根系酸铝胁迫与茶氨酸合成积累的相关性研究[D]. 合肥: 安徽农业大学, 2019.
Li Y Y.Study on the correlation between aluminum stress under acidic conditions and theanine synthesis and accumulation in roots of tea plant (Camellia sinensis L.) [D]. Hefei: Anhui Agricultural University, 2019.
[23] Ruan J Y, Gerendas J, Härdter R, et al.Effect of root zone pH and form and concentration of nitrogen on accumulation of quality-related components in green tea[J]. Journal of the Science of Food and Agriculture, 2007, 87(8): 1505-1516.
[24] Newman L, Duffus A L J, Lee C. Using the free program MEGA to build phylogenetic trees from molecular data[J]. The American Biology Teacher, 2016, 78(7): 608-612.
[25] Chen C J, Chen H, Zhang Y, et al.TBtools: an integrative toolkit developed for interactive analyses of big biological data[J]. Molecular Plant, 2020, 13(8): 1194-1202.
[26] Tang D D, Jiao Z X, Zhang Q F, et al.Glutamate dehydrogenase isogenes CsGDHs cooperate with glutamine synthetase isogenes CsGSs to assimilate ammonium in tea plant (Camellia sinensis L.)[J]. Plant Science, 2021, 312: 111031. doi: 10.1016/j.plantsci.2021.111031.
[27] 汤丹丹. 茶树胞质型谷氨酰胺合成酶基因CsGS1s的克隆及其对不同氮源的响应[D]. 北京: 中国农业科学院, 2018.
Tang D D.Isolation of cytosolic glutamine synthetase genes CsGS1s and their experssion in tea plant (Camellia sinensis L.) under different nitrogen forms [D]. Beijing: Chinese Academy of Agricultural Sciences, 2018.
[28] Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the ${{2}^{\text{-}\Delta \Delta {{\text{C}}_{\text{T}}}}}$ method[J]. Methods, 2001, 25(4): 402-408.
[29] Tian W H, Ye J Y, Cui M Q, et al.A transcription factor STOP1-centered pathway coordinates ammonium and phosphate acquisition in Arabidopsis[J]. Molecular Plant, 2021, 14(9): 1554-1568.
[30] Sun L, Di D W, Li G, et al.Endogenous ABA alleviates rice ammonium toxicity by reducing ROS and free ammonium via regulation of the SAPK9-bZIP20 pathway[J]. Journal of Experimental Botany, 2020, 71(15): 4562-4577.
[31] 彭文婷. 大豆GmSTOP1s基因家族的克隆和功能研究[D]. 广州: 华南农业大学, 2016.
Peng W T.Isolation and function analysis of GmSTOP1s gene family [D]. Guangzhou: South China Agricultural University, 2016.
[32] 韩庆芬, 陈海飞, 张振华. 不同生态型拟南芥耐铵毒害差异的生理机制[J]. 植物营养与肥料学报, 2019, 25(7): 1185-1193.
Han Q F, Chen H F, Zhang Z H, et al.Physiological mechanisms of tolerance to ammonium toxicity in different ecotypes of Arabidopsis thaliana[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(7): 1185-1193.
[33] Vega-Mas I, Rossi M T, Gupta K J, et al.Tomato roots exhibit in vivo glutamate dehydrogenase aminating capacity in response to excess ammonium supply[J]. Journal of Plant Physiology, 2019, 239(1): 83-91.
[34] Tokizawa M, Enomoto T, Ito H, et al.High affinity promoter binding of STOP1 is essential for early expression of novel aluminum-induced resistance genes GDH1 and GDH2 in Arabidopsis[J]. Journal of Experimental Botany, 2021, 72(7): 2769-2789.
[35] Liu M Y, Tang D D, Shi Y Z, et al.Short-term inhibition of glutamine synthetase leads to reprogramming of amino acid and lipid metabolism in roots and leaves of tea plant (Camellia sinensis L.)[J]. BMC Plant Biology, 2019, 19(1): 425. doi: 10.1186/s12870-019-2027-0.

Identification and Expression Pattern Analysis of STOP Gene Family in Tea Plants (Camellia sinensis)

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	ZHANG Shuqing, GUO Jinmei, LI Jianfeng, WU Ling, WANG Xi, ZENG Zhengqun. Effects of Phosphate Solubilizing Bacteriaand Phosphate-solubilizing and Nitrogen-fixing Bacteria on Selenium and Zinc Contents in Selenium-rich Soil and Camellia sinensis Seedlings in Guizhou [J]. Journal of Tea Science, 2024, 44(3): 431-442.
[2]	QIN Yujie, GUO Mingming, CHEN Yongjing, ZHOU Li. Determination of Afidopyropen and Metabolite M440I007 in Tea Tissues by Modified QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. Journal of Tea Science, 2024, 44(3): 515-525.
[3]	CUI Qingmei, LIANG Jinbo, MA Huijie, HU Shuangling, CHEN Qinghua, WU Liyun, HE Mengdi, WANG Liubin, TAN Licai, ZHANG Qiang, WANG Liyuan. Genetic Diversity and Population Structure Relationship Analysis of Wild Tea Germplasm Resources in Badong County, Hubei Province [J]. Journal of Tea Science, 2024, 44(2): 193-206.
[4]	SONG Bo, JIA Peining, YE Wenqi, WU Jun, SUN Weijiang, XUE Zhihui. Physiological Differences and Expression Analysis of Wax Synthesis Related Gene WSD1 in Tea Roots Treated with Fluorine [J]. Journal of Tea Science, 2024, 44(2): 219-230.
[5]	LI Qinghui, LI Rui, WEN Xiaoju, NI Dejiang, WANG Mingle, CHEN Yuqiong. Selection and Validation of Internal Reference Genes for qRT-PCR Analysis under Fluoride Stress in Camellia sinensis Leaves [J]. Journal of Tea Science, 2024, 44(1): 27-36.
[6]	WANG Liubin, WU Liyun, WEI Kang, WANG Liyuan. QTL Mapping and Candidate Gene Analysis for Timing of Spring Bud Flush in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2023, 43(6): 747-756.
[7]	LIU Dongna, GONG Xuejiao, LI Lanying, HUANG Fan, YAO Yu, XU Yaqiong, GAO Yuan, LUO Fan. Analysis of Photosynthetic and Fluorescence Characteristics of Albino Tea Plants [J]. Journal of Tea Science, 2023, 43(6): 757-768.
[8]	YANG Jun, ZHANG Lilan, ZHANG Wenjing, CHEN Linhai, ZHENG Guohua, LI Yijing, WANG Rangjian. Population Structure and Genetic Differences of Tea Germplasm Resources in Fujian [J]. Journal of Tea Science, 2023, 43(6): 769-783.
[9]	LI Yanchun, WANG Yixiang, YE Jing, LI Zhaowei. Changes of Rhizospheric Pathogen Alternaria sp. and Its Antagonistic Bacteria Pseudomonas sp. of Continuous Cropping Tea Plants Mediated by Phenolic Acids [J]. Journal of Tea Science, 2023, 43(6): 823-834.
[10]	YANG Jihong, ZHOU Hanchen, XU Yujie. Catalytic Function, Promoter Structure and Functional Analysis of CsNUDX1-cyto in Different Tea Cultivars [J]. Journal of Tea Science, 2023, 43(5): 621-630.
[11]	LIU Hongxia, LIU Yingying, CHEN Hongping, CHAI Yunfeng. Glyphosate-stress Effects on Shikimic Acid in Tea Leaves [J]. Journal of Tea Science, 2023, 43(5): 657-666.
[12]	TANG Ziyi, DU Yue, YANG Hongbin, LI Xinghui, YU Youben, WANG Weidong. Changes of Endogenous Hormone Contents and Expression Analysis of Related Genes in Leaves of Tea Plants Under Heat and Drought Stresses [J]. Journal of Tea Science, 2023, 43(4): 489-500.
[13]	HAN Haidong, ZHOU Liuting, HUANG Xiaoyun, YU Chengran, HUANG Xiusheng. The Characteristics of Fungal Community Structure in Tea Rhizosphere Soil Interplanted with Ganoderma lucidum Based on High-throughput Sequencing Technology [J]. Journal of Tea Science, 2023, 43(4): 513-524.
[14]	SUN Yue, LIU Mengyue, GAO Chenxi, WU Quanjin, CAO Shixian, YU Shuntian, CHEN Zhidan, JIN Shan, SUN Weijiang. Study on the Differences of Leaf Color and Volatiles of Different Insect-resistance Tea Cultivars [J]. Journal of Tea Science, 2023, 43(4): 525-543.
[15]	LI Jiasi, LIU Yingqing, ZHANG Yongheng, ZHANG Ying'ao, XIAO Yezi, LIU Lu, YU Youben. Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress [J]. Journal of Tea Science, 2023, 43(3): 325-334.