一体化QuEChERS净化-超高效液相色谱-串联质谱法测定茶叶中27种吡咯里西啶类生物碱

doi:10.13305/j.cnki.jts.2024.05.005

Abstract

Abstract: In this study, an integrated QuEChERS purification-ultra-performance liquid chromatography-tandem mass spectrometry method was established to detect 27 pyrrolizidine alkaloids (PAs) in tea, taking into account the residual risk of PAs in tea and the challenges of traditional QuEChERS. The method involves extracting the sample with 1% formic acid in acetonitrile, salting out using the centrifuge tube integrated salt bag and Down-Pressure QuEChERS Cleanup, detecting by multiple reaction monitoring mode (MRM), and quantifying it using the matrix-matched external standard method. The 27 PAs exhibit a strong linear relationship within their concentration ranges, with average recovery rates ranging from 73.0% to 111.8% and relative standard deviations from 1.6% to 13.8% (n=6). This method has the characteristics of simple operation, large detection throughput, high sensitivity, and reduction of repeated opening of the traditional QuEChERS, meeting the monitoring requirements for PAs in tea. During the actual testing of 60 batches of samples, different levels of PAs were detected in some tea samples, proving that there is indeed a risk of these plant toxins in tea, and continuous monitoring and tracking is recommended.

Key words: tea, UPLC-MS/MS, QuEChERS, pyrrolizidine alkaloids

CLC Number:

S571.1

YAO Leijun, CHEN Yanqiu, LIN Hao, WANG Luyao, SHI Peiyu, ZHANG Yangyang, HUANG Ting, SONG Juan, WANG Yi, DAI Qin, LIU Chuan. Simultaneous Determination of 27 Pyrrolizidine Alkaloids in Tea by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry with Integrated QuEChERS Purification[J]. Journal of Tea Science, 2024, 44(5): 831-842.

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Simultaneous Determination of 27 Pyrrolizidine Alkaloids in Tea by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry with Integrated QuEChERS Purification

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