绿茶、红茶和茯砖茶水提物对肠道微生物体外发酵特性的影响

doi:10.13305/j.cnki.jts.2019.04.005

Abstract

Abstract: In order to demonstrate the interaction effect of different tea aqueous extracts and intestinal microorganisms, anaerobic fermentation in vitro by the intestinal bacteria from healthy college students’ feces were conducted in this study. Traditional culture techniques and gas chromatography technology were employed to study the changes of pH, short-chain fatty acids and intestinal microbes in different tea aqueous extracts (green, black and Fu brick tea) during 24 h of in vitro fermentation, and calculate the prebiotic index PI and B/E values (Bifidobacterium/Enterobacterium). The results show that the tea aqueous extracts significantly decreased the pH value compared with the blank control group, and the biggest drop was identified in the Fu brick tea group. Besides, the tea aqueous extracts significantly increased the production of short-chain fatty acids. Green tea had the largest increase in acetic acid, propionic acid, isobutyric acid, iso-pentanoic acid, pentanoic acid and total short-chain fatty acids, while Fu brick tea had the largest increase in butyric acid. All three kinds of tea aqueous extracts could affect the composition of intestinal microorganisms, promoted the growth of Bifidobacterium and inhibited the growth of Bacteroides, Enterobacterium and clostridium. Fu brick tea group had the largest promoting effect on Bifidobacterium, had the most obvious inhibiting effect on Enterobacterium and clostridium, and green tea group had the largest inhibiting effect on Bacteroides. However, both green and black tea groups could promote the growth of Lactobacillus, while Fu brick tea group showed inhibition of Lactobacillus growth. In addition, by analyzing PI value and B/E value, It was found that green, black and Fu brick teas had the function as probiotics. The largest PI value at the 4 h stage appeared in fu brick tea, while the largest PI value at the 8 h, 12 h and 24 h stages appeared in green tea. the largest B/E value throughout the fermentation process appeared in Fu brick tea group. This study provided basic data and scientific basis for the further expansion and promotion of different teas in the youth consumption market.

Key words: green tea, black tea, Fu brick tea, in vitro fermentation, short-chain fatty acid, intestinal microbiota

CLC Number:

TS272

HOU Aixiang, YAN Daomin, SUN Jingwen, ZHENG Xu, LI Saidan, XIAO Runhua, BEI Shuang. Effects of Green, Black and Fu Brick Tea Aqueous Extracts on the Characteristics of Intestinal Microbiota during in vitro Fermentation[J]. Journal of Tea Science, 2019, 39(4): 403-414.

References 34

[1]	Clemente J C, Ursell L K, Parfrey L W, et al.The impact of the gut microbiota on human health: an integrative view[J]. Cell, 2012, 148(6): 1258-1270.
[2]	朱莹莹, 李春保, 周光宏. 饮食,肠道微生物与健康的关系研究进展[J]. 食品科学, 2015, 36(15): 234-239.
[3]	Cotillard A, Kennedy S P, Kong L C, et al.Dietary intervention impact on gut microbial gene richness[J]. Nature, 2013, 500(7464): 585-588.
[4]	Tremaroli V, Bäckhed F.Functional interactions between the gut microbiota and host metabolism[J]. Nature, 2012, 489(7415): 242-249.
[5]	刘月新, 叶良金. 茶多糖的研究进展[J]. 茶业通报, 2016(1): 38-43.
[6]	Chen G, Yuan Q, Saeeduddin M, et al.Recent advances in tea polysaccharides: Extraction, purification, physicochemical characterization and bioactivities[J]. Carbohydrate Polymers, 2016, 153: 663-678.
[7]	David L A, Maurice C F, Carmody R N, et al.Diet rapidly and reproducibly alters the human gut microbiome[J]. Nature, 2014, 505(7484): 559-563.
[8]	陈卫. 肠道菌群:膳食与健康研究的新视角[J]. 食品科学技术学报, 2015, 33(6): 1-6.
[9]	翟齐啸, 田丰伟, 王刚, 等. 肠道微生物与人体健康的研究进展[J]. 食品科学, 2013, 34(15): 337-341.
[10]	李娟, 活泼, 杨海燕. 茶叶功效成分研究进展[J]. 浙江科技学院学报, 2005(4): 285-289.
[11]	管曦, 杨江帆, 谢向英, 等. 基于CKB数据的中国茶叶消费行为研究[J]. 茶叶科学, 2018, 38(3): 287-295.
[12]	李家华, 卢晓慧, 蒋梦瑶, 等. 高校大学生茶叶消费情况与消费观念的调查分析[J]. 安徽农业科学, 2012, 40(34): 16903-16905.
[13]	蓝婷, 屠幼英. 在杭大学生对茶及茶产品的消费与剖析[J]. 中国茶叶加工, 2008(1): 13-16.
[14]	杜佩, 姜爱芹, 姜仁华, 等. 基于城市视角的年轻群体茶叶消费意愿与引导策略[J]. 中国茶叶加工, 2017, 3(4): 21-27
[15]	支梓鉴, 俞邱豪, 程焕, 等. 肠道微生物体外发酵模型研究进展及其在食品中的应用[J]. 食品工业科技, 2016, 37(14): 353-358.
[16]	Macfarlane G T, Hay S, Gibson G R.Influence of mucin on glycosidase, protease and arylamidase activities of human gut bacteria grown in a 3-stage continuous culture system[J]. Journal of Applied Microbiology, 1989, 66(5): 407-417.
[17]	Olano-Martin E, Mountzouris K C, Gibson G R, et al.In vitro fermentability of dextran, oligodextran and malt odextrin by human gut bacteria[J]. Br J Nutr, 2000, 83(3): 247-255.
[18]	Palframan R, Gibson G R, Rastall R A.Development of a quantitative tool for the comparison of the prebiotic effect of dietary oligosaccharides[J]. Letters in Applied Microbiology, 2003, 37(4): 281-284.
[19]	Van der Waaij D, Berghuisde Vries J M, Lekkerkerk L. Colonization resistance of the digestive tract in conventional and antibiotic-treated mice[J]. Journal of Hygiene, 1971, 69(3): 405-411.
[20]	Xin Zhang, Xiuling Zhu, Yongkang Sun, et al.Fermentation in vitro of EGCG GCG and EGCG3"Me isolated from Oolong tea by human intestinal microbiota[J]. Food Research International, 2013, 54(2): 1589-1595.
[21]	陈晓阳. 茯砖茶的生产与传播[J]. 茶叶通讯, 1997(1): 41-42.
[22]	傅冬和, 余智勇, 黄建安, 等. 不同年份茯砖茶水提物的抑菌效果研究[J]. 中国茶叶, 2011, 33(1): 10-12.
[23]	郭虹雯, 许翔雨, 陈莹, 等. 绿茶茶汤对肥胖相关肠道菌群的影响[J]. 茶叶科学, 2016, 36(4): 354-362.
[24]	吴根梁, 侯爱香, 李珂, 等. 陈年茯砖茶多酚类对老年人肠道菌群的影响研究[J]. 茶叶科学, 2018, 38(3): 319-330
[25]	刘平, 李宗军, 许爱清. 茯砖茶水提物对大肠杆菌感染小鼠的免疫调节作用[J]. 湖南农业大学学报(自然科学版), 2011, 37(5): 537-539.
[26]	岳随娟, 刘建, 龚加顺. 普洱茶茶褐素对大鼠肠道菌群的影响[J]. 茶叶科学, 2016, 36(3): 261-267.
[27]	郭虹雯, 许翔雨, 陈莹婕, 等. 绿茶茶汤对肥胖相关肠道菌群的影响[J]. 茶叶科学, 2016, 36(4): 354-362.
[28]	谢旻皓, 王晴川, 徐冬兰, 等. 苦丁茶冬青苦丁茶提取物与3,5-双咖啡酰奎尼酸对肠道微生物体外发酵的影响[J]. 食品科学, 2015, 36(17): 124-129.
[29]	Lesmes U, Emma J B, Gibson G R, et al.Effects of resistant starch type Ⅲ polymorphs on human colon microbiota and short chain fatty acids in human gut models[J]. Journal of Agricultural and Food Chemistry, 2008, 56(13): 5415-5421.
[30]	Bloemen J G, Venema K, van de Poll M C, et al. Short chain fatty acids exchange across the gut and liver in humans measured at surgery[J]. Clin Nutr(Edinburgh,Scotland), 2009, 28(6): 657-661.
[31]	Kim CH, Park J, Kim M.Gut microbiota-derived short-chain Fatty acids, T cells, and inflammation[J]. Immune Netw, 2014, 14(6): 277-288.
[32]	Vernazza C L, Gibson G R, Rastall R A.in vitro fermentation of chitosan derivatives by mixed cultures of human faecal bacteria[J]. Carbohydrate Polymers, 2005, 60(4): 539-545.
[33]	Sanchez-Patan F, Cueva C, Monagas M, et al.In vitro fermentation of a red wine extract by human gut microbiota: changes in microbial groups and formation of phenolic metabolites[J]. Journal of Agricultural and Food Chemistry, 2012, 60(9): 2136-2147.
[34]	郭贵海, 王崇文. 肠道菌群调节剂的研究进展[J]. 临床内科杂志, 2002, 19(2): 88-90.Jelena Vulevic, Robert A Rastall, Glenn R Gibson.Developing a quantitative approach for determining the in vitro prebiotic potential of dietary oligosaccharides Short communication[J]. FEMS Microbiology Letters, 2004, 236(1): 153-159.

Effects of Green, Black and Fu Brick Tea Aqueous Extracts on the Characteristics of Intestinal Microbiota during in vitro Fermentation

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