茶树CsANS基因及其启动子的克隆与生物信息学分析

doi:10.13305/j.cnki.jts.2016.02.014

Abstract

Abstract: The full length cDNA sequence of anthocyanin synthase gene（CsANS）was cloned from ‘Wuyi qizhong C18’ (Camellia sinensis) using rapid amplification of cDNA ends（RACE）technology. The promoter of CsANS was isolated using Genome Walking technology. The gene expression of ANS under different shading treatments was analyzed by the real-time PCR. The results showed that the full length cDNA of CsANS was 1 000 bp, and it contained a 957 bp open reading frame (ORF), which encoding a protein of 320 amino acid, including two conservative functional domains, DIOX-N and 20G-Fell-Oxy. A sequence containing 1 010 bp was isolated and found to be the promoter of CsANS, which contained many cis-acting elements related to anthocyanin biosynthetic pathway, such as the core promoter element TATA-box and light responsive element (including ACE, GT1-motif and Sp1), circadian (cis-acting regulatory element involved in circadian control). The real-time PCR analysis revealed that the gene was highly expressed under the sunshine (CK), while lowly expressed under the 75% shading. This study reveals that the expression of CsANS was regulated by light intensity.

Key words: Camellia sinensis, CsANS gene, promoter, bioinformatics analysis

CLC Number:

S571.1
Q52

JIN Qifang, CHEN Zhidan, SUN Weijiang, LIN Fuming, XUE Zhihui, HUANG Yan, TANG Xiuhua. Cloning and Bioinformatical Analysis of Anthocyanin Synthase Gene and Its Promoter in Camellia sinensis[J]. Journal of Tea Science, 2016, 36(2): 219-228.

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Cloning and Bioinformatical Analysis of Anthocyanin Synthase Gene and Its Promoter in Camellia sinensis

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