茯茶水提物对Ⅱ型糖尿病小鼠糖代谢紊乱的干预作用

doi:10.13305/j.cnki.jts.2016.03.004

Abstract

Abstract: In this study, streptozotocin (STZ) combined with a high-fat and high-sugar diet was used to generate a mouse model of type 2 diabetes mellitus. The aim of this study was to investigate the hypoglycemic effects of Fuzhuan brick tea water extract (FTEs) on T2DM-Mice. Results showed that FTEs could obviously alleviate weight loss, polyuria, polydipsia, and polyphagia, reduce fasting plasma glucose, increase sugar tolerance, enhance insulin concentrations and insulin sensitivity and decrease insulin resistance in the diabetic mice. Moreover, RT-qPCR analysis showed that FTEs promoted glucose transport and liver lipid metabolism, and improved the glucose metabolism in the diabetic mice by increasing the expression of PPAR-α, GLUT2, and GLUT4. Therefore, it can be concluded that FTEs could significantly improve glucose metabolism disorder symptom of T2DM-Mice by a dose-dependent manner.

Key words: Fuzhuan brick tea water extract, type 2 diabetes mellitus, hypoglycemic, glucose metabolism

CLC Number:

HUANG Song, LIU Zhonghua, HUANG Jian′an, YANG Heng, LI Qin. Intervention Effects of Fuzhuan Brick Tea Water Extract on Glucose Metabolism Disorder in a Mouse Model of Type Ⅱ Diabetes Mellitus[J]. Journal of Tea Science, 2016, 36(3): 250-260.

References 35

[1]	Yang W, Lu J, Weng J, et al.Prevalence of diabetes among men and women in China[J]. New England Journal of Medicine, 2010, 362(12): 1090-1101.
[2]	Huxley R, Lee CMY, Barzi F, et al.Coffee, decaffeinated coffee, and tea consumption in relation to incident type 2 diabetes mellitus: a systematic review with meta-analysis[J]. Archives of internal medicine, 2009, 169(22): 2053-2063.
[3]	Iso H, Date C, Wakai K, et al.The relationship between green tea and total caffeine intake and risk for self-reported type 2 diabetes among Japanese adults[J]. Annals of Internal Medicine, 2006, 144(8): 554-562.
[4]	Jing Y, Han G, Hu Y, et al.Tea consumption and risk of type 2 diabetes: a meta-analysis of cohort studies[J]. Journal of general internal medicine, 2009, 24(5): 557-562.
[5]	李布青,张慧玲,舒庆龄,等.中低档绿茶中茶多糖的提取及降血糖作用[J]. 茶叶科学, 1996, 16(1): 67-72.
[6]	Jian L, Xie LP, Lee AH, et al.Protective effect of green tea against prostate cancer: a case-control study in southeast China[J]. International Journal of Cancer, 2004, 108(1): 130-135.
[7]	Polychronopoulos E, Zeimbekis A, Kastorini C-M, et al.Effects of black and green tea consumption on blood glucose levels in non-obese elderly men and women from Mediterranean Islands (MEDIS epidemiological study)[J]. European journal of nutrition, 2008, 47(1): 10-16.
[8]	全吉淑, 尹学哲, 川和志. 茶多糖降糖作用机制[J]. 中国公共卫生, 2007, 23(3): 295-296.
[9]	王伟华, 韩占江. 茶多糖的提取纯化及其药理作用的研究进展[J]. 广东微量元素科学, 2007, 14(12): 8-13.
[10]	Wolfram S, Wang Y, Thielecke F.Anti-obesity effects of green tea: From bedside to bench[J]. Molecular nutrition & food research, 2006, 50(2): 176-187.
[11]	Whiting DR, Guariguata L, Weil C, et al.IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030[J]. Diabetes research and clinical practice, 2011, 94(3): 311-321.
[12]	文杰宇, 李宗军, 王远亮, 等. 黑茶中微生物及其相关保健功能研究进展[J]. 食品科学, 2010, 31(9): 329-332.
[13]	黄婧, 杨民和. 茯砖茶中“金花”菌的研究进展及应用潜力[J]. 福建茶叶, 2013, 35(1): 7-12.
[14]	傅冬和, 刘仲华, 黄建安, 等. 高通量筛选研究茯砖茶降脂功效[J]. 茶叶科学, 2006, 26(3): 209-214.
[15]	俞灵莺, 李向荣. 植物黄酮类抗糖尿病及其并发症的研究进展[J]. 国外医学: 卫生学分册, 2000, 27(6): 331-335.
[16]	郭芳彬. 花粉对糖尿病的疗效及机理探析[J]. 养蜂科技, 2003, 22(3): 25-29.
[17]	陈蓉, 宓文佳, 苏洁, 等. 大花紫薇提取物对STZ致Ⅱ型糖尿病小鼠的降糖作用研究[J]. 浙江中医药大学学报, 2014, 38(5): 517-520, 530.
[18]	李秀钧, 钱荣立. 胰岛素抵抗及其临床意义[J]. 中国糖尿病杂志, 1999, 7(3): 163-167.
[19]	邬云红, 李秀钧, 李宏亮, 等. 高脂饮食肥胖大鼠胰岛细胞胰岛素抵抗机理的探讨[J]. 中华医学杂志, 2005, 85(27): 1907-1910.
[20]	文晓红. 链脲佐菌素致胰岛破坏及其与高血糖关系的实验研究[D]. 重庆: 重庆医科大学, 2008: 24-27.
[21]	王怀清, 邹俊杰, 苏明丽, 等. 通络方药对糖尿病大鼠胰岛β细胞的保护作用[J]. 中国糖尿病杂志, 2009, 17(3): 178-180.
[22]	宋冰, 刘学政. 大黄素对KKAy糖尿病小鼠肝脏PPAR-γ及GluT-2表达的影响[J]. 军医进修学院学报, 2012, 33(7): 775-777, 790.
[23]	陈蔚, 陈雯洁, 夏燕萍, 等. 黄芪多糖对糖尿病仓鼠脂代谢紊乱及心肌PPAR-α表达的影响[J]. 复旦学报: 医学版, 2010, 37(2): 194-197, 215.
[24]	刘金苹. 糖尿病小鼠肝脏中STAT1、PPAR-γ和HGF的表达及丹参素的干预作用[D]. 济南: 山东大学, 2012: 27-31.
[25]	龙昭玲. PPARγ2和LDL-R基因与2型糖尿病脂代谢紊乱的相关性研究[D]. 贵阳: 贵阳医学院, 2014: 14-18.
[26]	罗强. 有氧运动和膳食控制对2型糖尿病大鼠Adiponectin、AMPK、GLUT-4的影响[D]. 扬州: 扬州大学, 2009: 14-28.
[27]	于姗. 不同血糖水平糖尿病大鼠心肌GLUT1和GLUT4mRNA表达的变化[D]. 济南: 山东大学, 2012: 17-18.
[28]	丁仁凤, 何普明, 揭国良. 茶多糖和茶多酚的降血糖作用研究[J]. 茶叶科学, 2005, 25(3): 219-224.
[29]	倪德江, 谢笔钧, 宋春和. 不同茶类多糖对实验型糖尿病小鼠治疗作用的比较研究[J]. 茶叶科学, 2002, 22(2): 160-163.
[30]	樊怡欣. 四种茶类对自发性2型糖尿病KKAy小鼠降糖作用的比较研究[D]. 北京: 北京中医药大学, 2011: 58-63.
[31]	欧阳规香, 郭则之. 茯砖茶发花技术研究[J]. 茶叶通讯, 1996, 35(2): 13-15.
[32]	李焱, 何娟, 李芳萍, 等. 抵抗素在肝脏胰岛素抵抗中的作用及其机制[J]. 中国病理生理杂志, 2009, 25(4): 688-692.
[33]	刘志红. 葡萄糖转运蛋白[J]. 肾脏病与透析肾移植杂志, 2000, 9(2): 153-158.
[34]	Kurosaki E, Nakano R, Shimaya A, et al.Differential effects of YM440 a hypoglycemic agent on binding to a peroxisome proliferator-activated receptor γ and its transactivation[J]. Biochemical pharmacology, 2003, 65(5): 795-805.
[35]	Lima FB, Thies RS, Garvey WT.Glucose and insulin regulate insulin sensitivity in primary cultured adipocytes without affecting insulin receptor kinase activity[J]. Endocrinology, 1991, 128(5): 2415-2426.

Intervention Effects of Fuzhuan Brick Tea Water Extract on Glucose Metabolism Disorder in a Mouse Model of Type Ⅱ Diabetes Mellitus

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