茯砖茶“金花”菌的分离、鉴定及转化葛根产物研究

doi:10.13305/j.cnki.jts.2016.03.006

Abstract

Abstract: In order to research whether the ′Jinhua′ fungus grow on and biotransformate kudzu root, get higher active fermentation products, one of advantages of strain was isolated from Fuzhuan Tea, named it as LS1. Pueraria lobata, its in the wild as the substrates in solid-state fermentation. DPPH radical scavenging capacities of fermentation products were investigated, making a determination of the contents of total flavone and fermentation products of puerarin were detected by high performance liquid chromatography. The result show that LS1 was identified preliminarily as Eurotium cristatum with GenBank accession number of KR812327 by morphological characteristics and the analysis of ITS-5.8S rDNA region. The contents of total flavone and puerarin were improved 15.97%、12.33%、20.69% and 20.48%、7%、20.29%, respectively.

Key words: ‘Jinhua’ fungus, kudzu root, fermentation, identification, antioxidant activity, puerarin

CLC Number:

HU Xiexin, YI Youjin, BO Lianyang, LI Gaoyang, ZHOU Hongli, ZHOU Jinwei, XIA Bo. Research on Separation, Identification and Transformation Products Transformed Kudzu Root of ′Jinhua′ Fungus[J]. Journal of Tea Science, 2016, 36(3): 268-276.

References 18

[1]	Loughlin W A.Biotransformation in organic synthesis[J]. Bioresource Technol, 2000, 74: 49-62.
[2]	Zhan J X, Zhong J J, Dai J G, et al.Microbial transformation of sinenxan A, a rich constituent in callus cultures of taxus[J]. Acta pharmaceutica Sinica, 2003, 38(7): 555-558.
[3]	唐燕红, 张日俊. 微生物在中草药添加剂中的应用研究进展[J]. 中国饲料, 2004(12): 20-21.
[4]	刘改霞. 葛根异黄酮的发酵提取工艺及其抗氧化性研究[D]. 南京: 南京农业大学, 2010.
[5]	张俊会. 葛根基质下食药用真菌深层液体发酵查无的抗氧化活性测定[D]. 保定: 河北大学, 2003.
[6]	宋艳秋, 陈有为. 葛根的红曲霉转化产物化学成分分析[J]. 天然产物研究与开发, 2013, 25(11): 1525-1528.
[7]	温琼英. 茯砖茶中主要微生物的研究[J]. 茶叶通讯, 1986(4): 19-21.
[8]	张琪, 污玉久, 张磊磊, 等. 两株具有将人参皂苷Rg1转化为人参皂苷F1活性的真菌鉴定[J]. 中国抗生素杂志, 2010, 35(5): 395-398.
[9]	黄婧. “金花”菌的分离鉴定及其对茶叶成分的生物转化[D]. 福州: 福建师范大学, 2013.
[10]	曾祥群. 葛根总黄酮提取工艺[J]. 食品工业科技, 2000, 21(3):33-34.
[11]	高宁. 盘龙参内生真菌的分离鉴定及其化学成分相关性的研究[D]. 哈尔滨: 黑龙江中医药大学, 2009.
[12]	Weiland J.Rapid procedure for the extraction of DNA from fungal spores and mycelia[J]. Fungal genetics newsletter, 1997, 44: 60-63.
[13]	陈雪. 不同类型茶叶抗氧化功能的比较研究[D]. 长沙: 湖南农业大学, 2012.
[14]	常飞, 段旭昌, 王倩倩. 不同干燥方法对葛根黄酮含量的影响研究[J]. 食品工业科技, 2014(35): 78-82.
[15]	刘华金, 易有金, 杨建奎, 等. 转化白藜芦醇苷虎杖内生真菌的分离和鉴定[J]. 食品科学, 2012, 33(11): 172-179.
[16]	齐组同. 中国真菌志: 曲霉属及其相关有性型: 第五卷[M]. 北京: 科学出版社, 1997: 139-142.
[17]	Raper K, Fennell D, Austwick P.The genus aspergillus[M]. Baltimore: Willians&Wilkins Baltimore, 1965: 20-23.
[18]	施英英, 夏黎明. 葛根渣固态发酵产异黄酮的研究[J]. 林产化学与工业, 2007, 27(1): 57-60.

Research on Separation, Identification and Transformation Products Transformed Kudzu Root of ′Jinhua′ Fungus

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