茶树两个Dof转录因子的分离及其在温度胁迫中的响应分析

doi:10.13305/j.cnki.jts.2016.03.011

Abstract

Abstract: Tea is an important economic crop. Tea production is affected by temperature factors. CsDof1 and CsDof2, which encode Dof transcription factors, were cloned from tea cultivar ‘Yingshuang’ [Camellia sinensis (L.) O. Kuntze]. The nucleotide and amino acid sequences, phylogenetic tree and molecular models of the CsDof1 and CsDof2 were analyzed. The results showed that the CsDof1 and CsDof2 are 1389 and 1458 nucleotides in length, which encode 463 and 486 amino acids respectively. Typical zinc finger domains are present in both CsDof1 and CsDof2, from coden 133 to 195 and 124 to 186 respectively. The CsDof1 and CsDof2 were hydrophilic proteins. The three-dimensional structures of CsDof1 and CsDof2 indicated the presence of a highly conserved Dof domain with four cysteine residues. Quantitative real-time PCR analysis showed that CsDof1 and CsDof2 were induced by different temperature stresses and the expression profiles were different among tea cultivars.

Key words: Camellia sinensis, Dof transcription factor, zinc finger, temperature stress, gene expression

CLC Number:

LI Hui, HUANG Wei, LIU Zhiwei, WANG Yongxin, WU Zhijun, ZHUANG Jing. Isolation and Expression Analysis of Two Temperature Responsive Dof Genes from Camellia sinensis[J]. Journal of Tea Science, 2016, 36(3): 312-322.

References 20

[1]	骆耀平. 茶树栽培学[M]. 北京: 中国农业出版社, 2008: 11-16.
[2]	宛晓春. 茶叶生物化学[M]. 北京: 中国农业出版社, 2003: 9-50.
[3]	刘欣, 李云. 转录因子与植物抗逆性研究进展[J]. 中国农学通报, 2006, 22(4): 61-65.
[4]	Umemura Y, Ishiduka T, Yamamoto R, et al.The Dof domain, a zinc finger DNA-binding domain conserved only in higher plants, truly functions as a Cys2/Cys2 Zn finger domain[J]. The Plant Journal, 2004, 37(5): 741-749.
[5]	Yanagisawa S, Izui K.Molecular cloning of two DNA-binding proteins of maize that are structurally different but interact with the same sequence motif[J]. Journal of Biological Chemistry, 1993, 268(21): 16028-16036.
[6]	Yanagisawa S.The Dof family of plant transcription factors[J]. Trends in Plant Science, 2002, 7(12): 555-560.
[7]	Lijavetzky D, Carbonero P, Vicente-Carbajosa J.Genome-wide comparative phylogenetic analysis of the rice and Arabidopsis Dof gene families[J]. BMC Evolutionary Biology, 2003, 3(1): 17.
[8]	Moreno-Risueno M Á, Martínez M, Vicente-Carbajosa J, et al. The family of DOF transcription factors: from green unicellular algae to vascular plants[J]. Molecular Genetics and Genomics, 2007, 277(4): 379-390.
[9]	Diaz I, Vicente-Carbajosa J, Abraham Z, et al.The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm-specific genes during seed development[J]. The Plant Journal, 2002, 29(4): 453-464.
[10]	Mena M, Cejudo F J, Isabel-Lamoneda I, et al.A role for the DOF transcription factor BPBF in the regulation of gibberellin-responsive genes in barley aleurone[J]. Plant Physiology, 2002, 130(1): 111-119.
[11]	Nakano T, Suzuki K, Ohtsuki N, et al.Identification of genes of the plant-specific transcription-factor families cooperatively regulated by ethylene and jasmonate in Arabidopsis thaliana[J]. Journal of Plant Research, 2006, 119(4): 407-413.
[12]	Yanagisawa S.Dof domain proteins: plant-specific transcription factors associated with diverse phenomena unique to plants[J]. Plant and Cell Physiology, 2004, 45(4): 386-391.
[13]	Wu Z J, Li X H, Liu Z W, et al.De novo assembly and transcriptome characterization: novel insights into catechins biosynthesis in Camellia sinensis[J]. BMC Plant Biology, 2014, 14(1): 277.
[14]	Schäffer A A, Aravind L, Madden T L, et al.Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements[J]. Nucleic Acids Research, 2001, 29(14): 2994-3005.
[15]	Tamura K, Peterson D, Peterson N, et al.MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 2011, 28(10): 2731-2739.
[16]	Combet C, Blanchet C, Geourjon C, et al.NPS@: network protein sequence analysis[J]. Trends in Biochemical Sciences, 2000, 25(3): 147-150.
[17]	Arnold K, Bordoli L, Kopp J, et al.The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling[J]. Bioinformatics, 2006, 22(2): 195-201.
[18]	Pfaffl M W.A new mathematical model for relative quantification in real-time RT-PCR[J]. Nucleic Acids Research, 2001, 29(9): 2002-2007.
[19]	Jung H J, Dong X, Park J I, et al.Genome-wide transcriptome analysis of two contrasting Brassica rapa doubled haploid lines under cold-stresses using Br135K Oligomeric Chip[J]. Plos One, 2013, 9(8): e106069.
[20]	Ma J, Li M Y, Wang F, et al.Genome-wide analysis of Dof family transcription factors and their responses to abiotic stresses in Chinese cabbage[J]. BMC Genomics, 2015, 16(1): 33.

Isolation and Expression Analysis of Two Temperature Responsive Dof Genes from Camellia sinensis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 20

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City [J]. Journal of Tea Science, 2022, 42(5): 601-609.
[2]	ZHOU Hanchen, YANG Jihong, XU Yujie, WU Qiong, LEI Pandeng. Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis [J]. Journal of Tea Science, 2022, 42(5): 638-648.
[3]	XING Anqi, WU Zichen, XU Xiaohan, SUN Yi, WANG Genmei, WANG Yuhua. Research Advances of Fluoride Accumulation Mechanisms in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 301-315.
[4]	WANG Tao, WANG Yiqing, QI Siyu, ZHOU Zhe, CHEN Zhidan, SUN Weijiang. Identification and Transcriptional Regulation of CLH Gene Family and Expression Analysis in Albino Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 331-346.
[5]	LIU Fuhao, FAN Yangen, WANG Yu, MENG Fanyue, ZHANG Lixia. Screening and Identification of Chaperone CsHIPP26.1 Chelating Ions in Tea Cultivar ‘Huangjinya’ [J]. Journal of Tea Science, 2022, 42(2): 179-186.
[6]	YANG Ni, LI Yimin, Li Jingwen, TENG Ruimin, CHEN Yi, WANG Yahui, ZHUANG Jing. Effects of Exogenous 5-ALA on the Chlorophyll Synthesis and Fluorescence Characteristics and Gene Expression of Key Enzymes in Tea Plants under Drought Stress [J]. Journal of Tea Science, 2022, 42(2): 187-199.
[7]	WANG Pengjie, YANG Jiangfan, ZHANG Xingtan, YE Naixing. Research Advance of Tea Plant Genome and Sequencing Technologies [J]. Journal of Tea Science, 2021, 41(6): 743-752.
[8]	ZHOU Hanchen, LEI Pandeng. The Functional Identification of Two Alternative Splicing Transcripts of CsNES [J]. Journal of Tea Science, 2021, 41(6): 753-760.
[9]	YAN Minghui, LIU Ke, WANG Man, LYU Ying, ZHANG Qian. Complete Chloroplast Genome of Camellia sinensis cv. Xinyang 10 and Its Phylogenetic Evolution [J]. Journal of Tea Science, 2021, 41(6): 777-788.
[10]	LIN Xinying, WANG Pengjie, CHEN Xuejin, GUO Yongchun, GU Mengya, ZHENG Yucheng, YE Naixing. Identification of LOX Gene Family in Camellia sinensis and Expression Analysis in the Process of White Tea Withering [J]. Journal of Tea Science, 2021, 41(4): 482-496.
[11]	WANG Yanding, WANG Huan, LI Nana, WANG Lu, HAO Xinyuan, WANG Yuchun, DING Changqing, YANG Yajun, WANG Xinchao, QIAN Wenjun. Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis [J]. Journal of Tea Science, 2021, 41(4): 497-510.
[12]	CHEN Siwen, KANG Rui, GUO Zhiyuan, ZHOU Qiongqiong, FENG Jiancan. Cloning and Expression Analysis of CsCML16 in Tea Plants (Camellia sinensis) under Low Temperature Stress [J]. Journal of Tea Science, 2021, 41(3): 315-326.
[13]	ZHAO Yiqing, LIU Zhengjun, ZHANG Tianxin, ZHAO Yanting, XIAO Bin, GAO Yuefang. Cloning of CsCHLI Gene and Its Expression Analysis in Different Albino Tea Cultivars (Camellia sinensis) [J]. Journal of Tea Science, 2021, 41(3): 327-336.
[14]	WAN Qiwen, YANG Ni, LI Yimin, HAN Miaohua, LIN Shijia, TENG Ruimin, ZHUANG Jing. Effects of Exogenous 24-Epibrassinolide on Photosynthetic Characteristics of Tea Plants [J]. Journal of Tea Science, 2021, 41(1): 58-70.
[15]	YAN Fei, JIANG Wenhua, QU Dong, FU Jing, ZHAO Xuan. Effects of Exogenous 5-aminolevulinic Acid on Photosynthetic and Physiological Characteristics of Tea Plants under Low Temperature Stress [J]. Journal of Tea Science, 2020, 40(5): 597-606.