Abstract: ：A method for correction coefficient of anthrone sulfuric acid to detect the polysaccharides in tea flower was established. According to the law of conservation of mass and normal anthrone sulfuric acid method determination of polysaccharide, correction coefficient for a new detection method of anthrone sulfuric acid (f) was constructed through the combined use of ion chromatography technology, which laid the foundation for the tea flower polysaccharide detection technology with fast, accurate and objective charcteristics. The results showed that the correction coefficient were 2.84 and 4.49 when galactose and glucose were used as standard reference substance respectively. The method had good stability (RSD_glucose=1.7%, RSD_galactose=1.0%), high precision (RSD_glucose=1.4%, RSD_galactose=2.0%), strong repeatability (RSD_glucose=3.2%, RSD_galactose=2.5%) and well recovery (recovery_glucose=91.3%-104.1%, recovery_galactose=95.9%-104.4%). Tea flower polysaccharide contents determined by the new method were 10.30%, 10.07% and 9.99% respectively in samples from Anhui, Sichuan and Zhejiang provinces, which were higher than the value determined by conventional anthrone sulfuric acid method. Monosaccharide composition analysis of polysaccharides in tea flower showed that galactose was better to be used as standard reference.

Key words: correction coefficient, anthrone-sulfuric acid method, polysaccharide, ion chromatography, tea flower