Abstract: In order to explore the absorption regularity of TF₁ and EGCG in the organism. This research used the Caco-2 cell monolayer model in vitro to simulate the absorption of TF₁ and EGCG in the small intestine. The influences of concentration and time on the absorption regularity of TF₁ and EGCG in Caco-2 cell monolayer model were investigated. The results illustrated that the absorption of TF₁ and EGCG in Caco-2 model showed that the apparent permeability coefficient raised with the increasing of concentrations of two compound in the range of 10~100βµmol·L^-1. The efflux rates of the TF₁ and EGCG showed the same rules as absorption. However, the increasing range of efflux rate was higher than that of absorption rate. The values of Papp about TF₁ and EGCG in the cell model were lower than 1×10^-6βcm·s ^-1, which indicated that both of them belonged to the kind of drugs which were difficult to absorb. However, with comparing the absorption rate, TF₁ was higher than EGCG in this model. Both of efflux transport showed the passive process because the efflux rates of TF₁ and EGCG were larger than 2 and higher than in cell model. Because the efflux regulation of TF₁ was in accordance with EGCG by temporal variation, it suggested that both of them were the substrate of the same efflux protein.

Key words: TF₁, EGCG, Caco-2 model, absorption regularity