茶黄素对BSA/MDA羰-氨交联反应体系中的蛋白质羰基化及其聚集化抑制作用研究

doi:10.13305/j.cnki.jts.2016.04.004

Abstract

Abstract: A model of age pigment generation was builted by carbonyl-ammonia cross linking reaction in vitro, the datas indicate that theaflavins can not only inhibit the generation of age pigment in protein carbonylation, but also supress β-sheet-rich in aggregate protein in this model by thioflavin T-based fluorometric assay, scanning electron microscope, transmission electric microscope, SDS-PAGE and NBT staining, there are postive relationship between protein carbonylation on mechanism of aging and protein aggregation that lead to neurodegenerative disease.

Key words: theaflavin, carbonyl-ammonia cross linking reaction, age pigment, protein aggregation

CLC Number:

ZHANG Jing, CAI Shuxian, HUANG Jian'an, LI Juan, ZHONG Ni, YANG Heng, LIU Zhonghua. Inhibition of Theaflavins on Protein Carbonylation and Aggregation in the System of BSA/MDA Carbonyl-ammonia Cross Linking Reaction in vitro[J]. Journal of Tea Science, 2016, 36(4): 363-371.

References

[1] Chen N, Bezzina R, Hinch E, et al.Green tea, black tea, and epigallocatechin modify body composition, improve glucose tolerance, and differentially alter metabolic gene expression in rats fed a high-fat diet[J]. Nutrition Research, 2009, 29(11): 784-793.
[2] Kundu T, Dey S, Roy M, et al.Induction of apoptosis in human leukemia cells by black tea and its polyphenol theaflavin[J]. Cancer Letters, 2005, 230(1): 111-121.
[3] Friedman M, Henika P R, Levin C E, et al.Antimicrobial activities of tea catechins and theaflavins and tea extracts against Bacillus cereus[J]. Journal of Food Protection, 2006, 69(2): 354-361.
[4] Bastianetto S, Yao Z X, Papadopoulos V, et al.Neuroprotective effects of green and black teas and their catechin gallate esters against β-amyloid-induced toxicity[J]. European Journal of Neuroscience, 2006, 23(1): 55-64.
[5] Yin D.Biochemical basis of lipofuscin, ceroid, and age pigment-like fluorophores[J]. Free Radical Biology and Medicine, 1996, 21(6): 871-888.
[6] Yin D.Studies on age pigments evolving into a new theory of biological aging[J]. Gerontology, 1995, 41(Supp. 2): 159-172.
[7] 陈刚领, 唐宁, 薛永亮. 蛋白质羰基化与疾病关系的研究进展[J]. 辽宁中医药大学学报, 2009, 11(7): 58-60.
[8] Roth M, Tomlinson B, Blessed G.The relationship between quantitative measures of dementia and of degenerative changes in the cerebral grey matter of elderly subjects[J]. Proceedings of the Royal Society of Medicine, 1967, 60(3): 254.
[9] Ahmad N, Gupta S, Mukhtar H.Green tea polyphenol epigallocatechin-3-gallate differentially modulates nuclear factor kappaB in cancer cells versus normal cells[J]. Archives of Biochemistry and Biophysics, 2000, 376(2): 338-346.
[10] Caruana M, Neuner J, Hogen T, et al.Polyphenolic compounds are novel protective agents against lipid membrane damage by α-synuclein aggregates in vitro[J]. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2012, 1818(11): 2502-2510.
[11] Katiyar S K, Bergamo B M, Vyalil P K, et al.Green tea polyphenols: DNA photodamage and photoimmunology[J]. Journal of Photochemistry and Photobiology B, Biology, 2001, 65(2/3): 109-114.
[12] Cheng K W, Wong C C, Chao J, et al.Inhibition of mutagenic PhIP formation by epigallocatechin gallate via scavenging of phenylacetaldehyde[J]. Molecular Nutrition & Food Research, 2009, 53(6): 716-725.
[13] Sang S, Shao X, Bai N, et al.Tea polyphenol (-)-epigallocatechin-3-gallate: a new trapping agent of reactive dicarbonyl species[J]. Chemical Research in Toxicology, 2007, 20(12): 1862-1870.
[14] Zhu Q, Liang C P, Cheng K W, et al.Trapping effects of green and black tea extracts on peroxidation-derived carbonyl substances of seal blubber oil[J]. Journal of Agricultural and Food Chemistry, 2009. 57(3): 1065-1069.
[15] 蔡淑娴, 刘仲华, 黄建安, 等. 儿茶素抑制蛋白质类老年色素荧光物质生成活性的研究[J]. 茶叶科学, 2010, 30(1): 1-8.
[16] Riviere C, Richard T, Vitrac X, et al.New polyphenols active on β-amyloid aggregation[J]. Bioorganic & Medicinal Chemistry Letters, 2008, 18(2): 828-831.
[17] 江和源, 袁新跃, 王川丕. 茶黄素双没食子酸酯的抗癌活性及其作用机理研究[J]. 茶叶科学, 2007, 27(1): 33-38.
[18] 李国林, 印大中. 蛋白质羰基化与衰老[J]. 中国老年学杂志, 2008, 28(20): 2070-2073.

Inhibition of Theaflavins on Protein Carbonylation and Aggregation in the System of BSA/MDA Carbonyl-ammonia Cross Linking Reaction in vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	ZHOU Yufei, ZHOU Lin, YU Lijun, XU Shuai, YANG Yihuan. Visualization Analysis in Research Status of Theaflavins Based on CiteSpace [J]. Journal of Tea Science, 2022, 42(1): 131-139.
[2]	DENG Zhihui, ZENG Jie, FU Hongjuan, CHANG Hui. Effects of Theaflavin-3,3'-O-Digallate on Vascular Endothelial Injury and Inflammation in Rats with Diabetes Mellitus [J]. Journal of Tea Science, 2021, 41(6): 823-830.
[3]	ZENG Jie, DENG Zhihui, FU Hongjuan, LIU Chang, GU Yi, ZOU Yixin, CHANG Hui. Theaflavin Activates Nrf2/HO-1 Pathway to Alleviate Oxidative Stress Injury in Vascular Endothelial Cells [J]. Journal of Tea Science, 2020, 40(5): 632-640.
[4]	ZHANG Jing, HUANG Jian'an, CAI Shuxian, YI Xiaoqin, LIU Jianjun, WANG Yingzi, TIAN Lili, LIU Zhonghua. Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ₄₂ in vivo and in vitro [J]. Journal of Tea Science, 2016, 36(6): 655-662.
[5]	XU Bin, JIANG Heyuan, ZHANG Jianyong, YANG Liuyan, LIU Qianlu. Formation Mechanism of TSs and Competitive Formation Between TSs and TFs under Various pH [J]. Journal of Tea Science, 2015, 35(3): 281-289.
[6]	CHEN Dongsheng, WANG Kunbo, LI Qin, LI Juan, HUANG Jian′an, LIU Zhonghua. The Cloning and Prokaryotic Expression of Polyphenol Oxidase Gene in Pear (Pyrus Pyrifolia Nakai) [J]. Journal of Tea Science, 2015, 35(1): 17-23.
[7]	XU Bin, XUE Jinjin, JIANG Heyuan, ZHANG Jianyong, WANG Yan. Review on Theasinensins in Tea [J]. Journal of Tea Science, 2014, 34(4): 315-323.
[8]	ZHANG Cong, TAN Rong, KONG Junhao, GE Qingfeng, YANG Xiufang, WANG Zhijun. Research on the Antihyperlipdemic Activity of Theaflavins, Catechins and Saponius Mixture Preparations [J]. Journal of Tea Science, 2014, 34(4): 401-407.
[9]	GONG Zhi-hua, CHEN Dong, ZHU Sheng-rao, CHEN Lin, CHEN Duo, LI Xu, XIAO Wen-Jun. Screening of the Sources of Enzymes from Fresh Tea Leaves for the Enzymatic Synthesis of Theaflavins [J]. Journal of Tea Science, 2013, 33(1): 34-39.
[10]	YU Jun, WANG Li-pu, YUE Peng-xiang, FANG Wan-ping, CHEN Xuan, LI Xing-hui. Studies on Purifying Theaflavins with Three Macroporous Adsorptive Resins [J]. Journal of Tea Science, 2011, 31(5): 447-452.
[11]	LIU Xiao-dong, LIU Yu-fang, GAN Chun-ping, YANG Chun, TANG Zhou-bin. Research on Tea Liquor Variation in Terms of Light Absorption Value during Black Tea Fermentation Process [J]. Journal of Tea Science, 2011, 31(4): 300-304.
[12]	CAI Shu-xian, LIU Zhong-hua, HUANG Jian-an, LUO Guo-an, YU Xing-long, DONG Xin-rong, YANG Zhi-hui, YE Fei. Study on Inhibiting Effects of Tea Catechins on the Formation of Age Pigment Fluorescence Products [J]. Journal of Tea Science, 2010, 30(1): 1-8.
[13]	LI Cai-rong, CAI Fei, ZHAO Xin-yuan, YANG Yin-qiao. Effect of Theaflavin on the Activity of p38 Mitogen-activated Protein Kinase (p38MAPK) and Extracellar Matrix Production in Rat Mesangial Cells [J]. Journal of Tea Science, 2009, 29(6): 470-474.
[14]	WANG Wen-xiang, SU Chang, LIAO Hui-zhen. Effect of Tea Pigments on Insulin Receptor in Insulin Resistant Rats [J]. Journal of Tea Science, 2008, 28(6): 455-458.
[15]	LI Shi, LIU Zhong-hua, HUANG Jian-an, WANG Kun-bo, SHI Ling. Research on Enzymatic Nature of Polyphenol Oxidase from Trametes Trogii and the Enzymatic Synthesis of Theaflavins [J]. Journal of Tea Science, 2008, 28(5): 326-330.