茶树硝态氮转运蛋白NRT1.1基因的克隆及表达分析

doi:10.13305/j.cnki.jts.2016.05.009

Abstract

Abstract: A full length cDNA sequence of Nitrate transport gene (NRT1.1) was obtained from tea plant (Camellia sinensis (L.)) cultivar ‘Longjing 43’ by polymerase chain reaction (PCR) and rapid amplification of cDNA ends PCR (RACE-PCR). The length of nucleotide sequence of this gene was 1 880 bp, containing a complete open reading frame (1 788 bp) to encode 595 amino acids. The putative protein had an isoelectric point of 8.99 and a calculated molecular weight of 65.9 kD. CsNRT1.1 was highly homologous to the gene NRT1.1 in Vitis vinifera by sequence alignment. Several parameters of these sequences, including sequences composition, physicochemical property, topological structure of transmembrane regions, hydrophobicity or hydrophilicity, subcellular localization were predicted by bioinformatics tools. Quantitative real-time PCR analysis showed that the expression of CsNRT1.1 in roots and leaves were inhibited after incubation in 1 mol·L^-1 NO₃^- for 5 min. The expressions of CsNRT1.1 in roots were always lower than that of CK within 24 h. Its expressions in leaves were higher than those in roots with its peak

Key words: Camellia sinensis, nitrate, NRT1.1, RT-PCR

CLC Number:

S571.1
Q51

YANG Yiyang, HU Yunfei, WAN Qing, LI Ronglin, WANG Feng, RUAN Jianyun. Cloning and Expression Analysis of Nitrate Transporter NRT1.1 Gene in Tea Plant (Camellia sinensis (L.))[J]. Journal of Tea Science, 2016, 36(5): 505-512.

References 19

[1]	Kamau D M, Spiertz J H J, Oenema O, et al. Productivity and nitrogen use of tea plantations in relation to age and genotype[J]. Field Crops Research, 2008, 108(1): 60-70.
[2]	杜旭华, 彭方仁. 无机氮素形态对茶树氮素吸收动力学特性及个体生长的影响[J]. 作物学报, 2010, 36(2): 327-334.
[3]	Yang Y Y, Li X H, Ratcliffe R G, et al.Characterization of ammonium and nitrate uptake and assimilation in roots of tea plants[J]. Russian Journal of Plant Physiology, 2013, 60(1): 91-99.
[4]	Dechorgnat J, Nguyen C T, Armengaud P, et al.From the soil to the seeds: the journey of nitrate in plants[J]. Journal of Experimental Botany, 2010, 62(4): 1349-1359.
[5]	Gojon A, Krouk G, Perrine-Walker F, et al.Nitrate transceptor(s) in plants[J]. Journal of Experimental Botany, 2011, 62(7): 2299-2308.
[6]	Okamato M, Vidmar J J, Glass A D M. Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana: responses to nitrate provision[J]. Plant Cell Physiology, 2003, 44(3): 304-317.
[7]	Orsel M, Krapp A, Daniel-Vedele F.Analysis of the NRT2 nitrate transporter family in Arabidopsis. Structure and gene expression[J]. Plant Physiology, 2002, 129(2): 886-896.
[8]	Araki R, Hasegawa H.Expression of rice (Oryza sativa L.) genes involved in high-affinity nitrate transport during the period of nitrate induction[J]. Breeding Science, 2006, 56(3): 295-302.
[9]	冯素花. 茶树硝酸根转运蛋白NRT1.2、NRT1.5和NRT2.5基因的克隆和表达[D]. 杭州: 中国农业科学院茶叶研究所, 2014: 24-25.
[10]	汪进, 添先凤, 江昌俊, 等. 茶树硝酸盐转运蛋白基因的克隆和表达分析[J]. 植物生理学报, 2014, 50(7): 983-988.
[11]	Tsay Y F, Schroeder J I, Feldmann K A, et al.The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate- inducible nitrate transporter[J]. Cell, 1993, 72: 705-713.
[12]	Wang R C, Liu D, Crawford N M.The Arabidopsis CHL1 protein plays a major role in high-affinity nitrate uptake[J]. Proceedings of the national academy of sciences, 1999, 95(25): 15134-15139.
[13]	Anthony D M, Glass Z K.A reevaluation of the role of Arabidopsis NRT1.1 in high-affinity nitrate transport[J]. Plant physiology, 2013, 163: 1103-1106.
[14]	Tsay Y F, Chiu C C, Tsai C B, et al.Nitrate transporters and peptide transporters[J]. FEBS Letters, 2007, 581: 2290-2300.
[15]	Wang R C, Okamoto M, Xing X J, et al.Microarray analysis of the nitrate response in Arabidopsis roots and shoots reveals over 1000 rapidly responding genes and new linkages to glucose, trehalose-6-phosphate, iron, and sulfate metabolism[J]. Plant Physiology, 2003, 132(2): 556-567.
[16]	Wang R C, Xing X J, Crawford N M.Nitrate acts as transcriptome signal at micromolar concentrations in Arabidopsis roots[J]. Plant Physiology, 2007, 145(4): 1735-1745.
[17]	孔敏, 杨学东, 侯喜林, 等. 白菜NRT2基因的克隆及表达模式分析[J]. 园艺学报, 2011, 38(12): 2309-2316.
[18]	Cardenas-Navarro R, Adamowicz S, Robin P.Diurnal nitrate uptake in young tomato (Lycopersicon esculentum Mill) plants: test of a feedback-based model[J]. Journal of Experimental Botany, 1998, 49(321): 721-730.
[19]	徐海荣, 谷俊涛, 路文静, 等. 水稻硝酸盐转运蛋白基因OsTNrt2.1的编码蛋白特征和表达[J]. 作物学报, 2007, 33(5): 723-730.

Cloning and Expression Analysis of Nitrate Transporter NRT1.1 Gene in Tea Plant (Camellia sinensis (L.))

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