一种毛尖茶叶多糖MTP06的提取分离及其活性测定

doi:10.13305/j.cnki.jts.2016.05.012

Abstract

Abstract: To investigate the structure and activity of polysaccharides (MTP06), the polysaccharides were extracted and purified from Maojian tea. The refined Maojian tea polysaccharides (MP) were obtained after removing proteins. Different column chromatography methods were used for several times to purify the MP and an uniform polysaccharide named MTP06 was obtained. The effects of MTP06 on DPPH radical-scavenging activity, cell viability, engulfment capability and NO production of RAW264.7 macrophages were investigated . The results showed that the free radical scavenging rate of MTP06 to 0.1 mmol·L^-1DPPH solution was 30.85%. The highest viability, engulfment capability and NO production of RAW264.7 macrophages had been stimulated by MTP06 at 500 mg·L^-1. The present results confirmed MTP06 can eliminate DPPH radicals and stimulate cell viabilities.

Key words: Maojian tea, polysaccharides, activity, macrophages

CLC Number:

TS272
Q539

FAN Haitao, LIU Zhuang, ZHANG Zhaokang, CHENG Xiangyi, QIAO Shanyi. Purification and Activity Determination of Polysaccharides MTP06 from Maojian Tea[J]. Journal of Tea Science, 2016, 36(5): 531-536.

References 12

[1]	Baruch Narotzki, Abraham Z Reznick, Dror Aizenbud, et al.Green tea:a promising natural product in oral health[J]. Arch Oral Biol, 2012, 57(5): 429-435.
[2]	全吉淑, 尹学哲, 金泽武道. 茶多糖抗氧化作用的研究[J]. 中药材, 2007, 30(9): 1116-1118.
[3]	Mateos Aparicio, C Mateos Peinado, A Jiménez Escrig, et al. Multifunctional antioxidant activity of polysaccharide fractions from the soybean byproduct okara[J]. Carbohydrate Polymers, 2010, 82(2): 245-250.
[4]	赵津, 常军民, 郑杰, 等. 刺糖多糖免疫活性的初步研究[J]. 西北药学杂志, 2016, 31(2): 158-161.
[5]	Wang Y, Yang Z, Wei X.Antioxidant activities potential of tea polysaccharide fractions obtained byultra filtration[J]. Int J Biol Macromol, 2012, 50(3): 558-564.
[6]	Chen X, Wang Y, Wu Y, et al.Green tea polysaccharide-conjugates protect human umbilical vein endothelialcells against impairments triggered by high glucose[J]. Int J BiolMacromol, 2011, 49(1): 50-54.
[7]	陈仕学, 田艺, 卢忠英, 等. 响应面法优化都匀毛尖茶多糖的提取及抗氧化性研究[J]. 食品工业, 2015, 36(9): 83-88.
[8]	曲伟, 王孟君, 于海燕, 等. 毛尖茶叶多糖及其结合物的抗氧化活性研究[J]. 天然产物研究与开发, 2015, 27(2): 210-213.
[9]	白娣斯, 张静. 气相色谱分析多糖衍生化方法的研究与比较[J]. 食品工业科技, 2011, 32(2): 322-324.
[10]	吴玉娟, 王懿萍, 姜延伟, 等. 黄连多糖含量测定及抗氧化活性研究[J]. 时珍国医国药, 2008, 19(8): 1906-1908.
[11]	蔡海兰, 黄晓君, 聂少平, 等. 铁皮石斛多糖对RAW264.7细胞分泌TNF-α的影响[J]. 中国药理学通报, 2012, 28(11): 1553-1556.
[12]	武俊华, 罗成. Viili多糖对巨噬细胞RAW264.7激活及分泌细胞因子的影响[J]. 现代免疫学, 2013, 33(2): 113-118.

[1]	ZHANG Zhipeng, CHENG Qinghua, XIE Jingkang, WAN Yuhe, TONG Huarong, CHEN Yingjuan. The Antifungal Effect and Mechanism of Berberine on Different Colletotrichum Species Causing Tea Brown Blight Disease [J]. Journal of Tea Science, 2022, 42(3): 367-375.
[2]	YANG Gaozhong, PENG Qunhua, ZHANG Yue, SHI Jiang, LIN Zhi, LÜ Haipeng. Effects of Anaerobic Treatment on Amino Acid Composition and Biological Activities of Different Type Teas [J]. Journal of Tea Science, 2022, 42(2): 222-232.
[3]	LIU Jianjun, LI Meifeng, ZHANG Jing, TIAN Lili, DENG Yanli, LIN Haiyan, HUANG Jian′an, LIU Zhonghua. A Study of Xinyang Maojian Tea Water Extracts on Relieving Lung Injury Caused by PM_2.5 [J]. Journal of Tea Science, 2021, 41(4): 525-534.
[4]	ZHI Yanan, XU Yunfei, ZHU Mingxing, WANG Chunsheng, JIN Yinli, CHEN Lijun. Volatile Organic Compounds from Fresh Tea Leaves and Their Fumigation Antifungal Activities on Fungal Pathogens of Tea Plants [J]. Journal of Tea Science, 2021, 41(3): 371-378.
[5]	MA Wanjun, MA Shicheng, LIU Chunmei, LONG Zhirong, TANG Baojun, LIN Zhi, LYU Haipeng. Research Progress on Chemical Composition and Biological Activity of Liupao Tea [J]. Journal of Tea Science, 2020, 40(3): 289-304.
[6]	LIU Danqi, REN Fazheng, LI Jingming, HOU Caiyun. Comparative Study on the Structure and Hypoglycemic Activity of Several Tea Polysaccharides [J]. Journal of Tea Science, 2019, 39(6): 652-660.
[7]	ZHU Lin, WU Long, CHEN Xiaoqiang, CHEN Xueling, WU Zhengqi, SHI Yong. Interaction between Tea Polyphenols and Polysaccharides: Progress in Research on Mechanism and Function [J]. Journal of Tea Science, 2019, 39(2): 203-210.
[8]	GAN Yudi, SUN Kang, LI Huijuan, DU Zhongying, ZHAO Zhen, PANG Xing, LI Xinghui, CHEN Xuan. Effect of Two Prokaryotic Expressed Vectors on the Activity of PPO from Camellia sinensis [J]. Journal of Tea Science, 2018, 38(4): 396-405.
[9]	WANG Qizhi, LIU Yumei, LI Shuming. The Repellency and Fumigant Activity of Essential Oil from Invasive Plant (Ageratum conyzoides) against Leafhopper (Empoasca onukii) [J]. Journal of Tea Science, 2017, 37(5): 442-448.
[10]	WANG Le, LI Huan, LI Jiahao, CHEN Xuan, LI Xinghui, SUN Kang. The Evaluation of the Stability of EGCG-Selenium Nanoparticles and Its Effect on Selenium Absorption and Utilization [J]. Journal of Tea Science, 2017, 37(4): 373-382.
[11]	YING Le, PAN Yue, WANG Yuefei, XU Ping. Physicochemical Properties, in vitro Antioxidant Activities and Protective Effects of Liubao Tea Polysaccharides on HUVEC [J]. Journal of Tea Science, 2017, 37(1): 25-37.
[12]	LIU Jianjun, LI Meifeng, ZHANG Jing, TIAN Lili, WANG Kunbo, HUANG Jian′an, LIU Zhonghua. Effects of Traditional and Modern Processing Technologies on the Quality of Xinyang Maojian Tea [J]. Journal of Tea Science, 2016, 36(6): 594-602.
[13]	WEI Nan, ZHU Qiangqiang, CHEN Jiming, LI Tong, LI Yifan, HUANG Yewei, WANG Xuanjun, SHENG Jun. Tea Polysaccharide Increased Doxorubicin Inhibition of Lung Cancer A549 Cells [J]. Journal of Tea Science, 2016, 36(5): 477-483.
[14]	HU Xiexin, YI Youjin, BO Lianyang, LI Gaoyang, ZHOU Hongli, ZHOU Jinwei, XIA Bo. Research on Separation, Identification and Transformation Products Transformed Kudzu Root of ′Jinhua′ Fungus [J]. Journal of Tea Science, 2016, 36(3): 268-276.
[15]	LIU Min, RAO Guowu, HUA Yunfen. Research Advance in Synthesis and Pharmacological Effects of EGCG Derivatives [J]. Journal of Tea Science, 2016, 36(2): 119-130.

Purification and Activity Determination of Polysaccharides MTP06 from Maojian Tea

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 12

Related Articles 15

Metrics

Comments

Recommended 10