发根农杆菌抑菌剂的抑菌效果及对茶组培苗丛生芽的影响

doi:10.13305/j.cnki.jts.2015.05.005

Abstract

Abstract: Effects of three antibiotics on inhibition of Agrobacterium Rhizogenes LBA4404 and 15834, bud formation and growth of tea plant were studied. The results showed that cefotaxime sodium and timentin can completely inhibit the growth of Agrobacterium LBA9402 and 15834, however, carbenicillin only inhibit LBA9402 bacterium but not 15834 bacterium. Bud regeneration and growth are significantly inhibited by cefotaxime sodium and carbenicillin treatment. There is no significant difference between Timentin (≤400βmg·L^-1) treatment and control without antibiotics on bud regeneration and growth of tea culture seedlings. Timentin is a good alternative antibiotic to eliminate Agrobacterium tumefaciens in tea plant genetic transformation system.

Key words: Camellia sinensis, Agrobacterium, antibiotic, physiology

CLC Number:

YANG Yaping, LI Yonglan, LIANG Yuerong, LU Jianliang, ZHENG Xinqiang. Antibiotics Inhibition to Agrobaceterium Rhizogenes and Effect to Tea Multiple Shoots[J]. Journal of Tea Science, 2015, 35(5): 437-442.

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Antibiotics Inhibition to Agrobaceterium Rhizogenes and Effect to Tea Multiple Shoots

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