茶树磷酸烯醇式丙酮酸转运子基因CsPPT的克隆与表达分析

doi:10.13305/j.cnki.jts.2015.05.014

Abstract

Abstract: The open reading frame (ORF) of CsPPT (GenBank accession number: KJ652972) from Camellia sinensis ‘Baiye 1’was obtained using RT-PCR and RACE technology. The deduced ORF of CsPPT has 1 227 nucleotides, encoding 408 amino acids. Bioinformatic analysis showed that the molecular weight of the predicted protein was 44.7 kDa, and the theoretic isoelectric point was 10.16. The predicted protein had no signal peptide, belonging to non-secretory protein. Poylogenetic tree of CsPPT had been built. Phosphorylation prediction showed the whole protein polypeptide contained 26 phosphorylation sites. Subcellular localization of CsPPT protein displayed that it was located in the chloroplast. TMHMM analysis showed that CsPPT protein belonged to transmembrane proteins. QRT-PCR analysis results showed that the expression of CsPPT has obvious tissue specificity, which was higher in flower, followed by buds, leaves, and tender stems, but lower in roots.

Key words: Camellia sinensis, phosphoenolpyruvate transporter, gene cloning, subcellular localization, expression analysis

CLC Number:

S571.1
Q52

ZHAO Zhen, CHEN Xuan, WANG Mingle, WANG Weidong, Najeeb Ahmed, LI Xinghui. Cloning and Expression Analysis of Phosphoenolpyruvate Transporter Gene CsPPT from Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2015, 35(5): 491-500.

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Cloning and Expression Analysis of Phosphoenolpyruvate Transporter Gene CsPPT from Tea Plant (Camellia sinensis)

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