茶树叶片氟亚细胞分布及其与细胞壁结合特性的研究

doi:10.13305/j.cnki.jts.2018.03.011

Abstract

Abstract: Tea plant (Camellia sinensis) is a hyper-accumulator of fluorine (F) and its leaves are the main accumulating tissue. In this paper, the F subcellular distribution and its binding characteristics with cell wall in tea leaves were investigated to explore the characteristics of F accumulating in tea leaves. The results showed that most of the F was distributed in the cell wall (39.74%-56.49%) and soluble fraction (28.35%-37.32%) of leaves. Pectin and hemicellulose are the main components of F enrichment and accumulated more than 90% of F in cell wall. There is a significant and positive correlation between Mn²⁺, Ca²⁺, Al³⁺ and F. The contents of F and metal elements (Mn²⁺, Fe³⁺, Ca²⁺, Mn²⁺, Al³⁺) reduced significantly in methylation and esterification cell wall while changed little in enzymolysis cell wall. These suggested that -NH₂ and -COOH might play an important role in the combination of F and cell wall, while the molecule weights of pectin and cellulose played little role. Cell wall and soluble fraction are the main sites to store F where F might be combined with -OH, -NH₂and -COOH in the form of hydrogen bonds or with metal elements in the form of ionic bonds. F might be fixed in cell wall and vacuolar as the main defense mechanism of tea plant against F toxicity.

Key words: tea plant, fluorine, subcellular distribution, cell wall, combining characteristics

CLC Number:

S571.1

LIU Siyi, ZHU Xiaojing, FANG Fengxiang, ZHANG Haojie, QIU Andong, CHEN Yuqiong*. Fluorine Subcellular Distribution and Its Combining Characteristics with Cell Wall in Tea Leaves (Camellia sinensis)[J]. Journal of Tea Science, 2018, 38(3): 305-312.

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Fluorine Subcellular Distribution and Its Combining Characteristics with Cell Wall in Tea Leaves (Camellia sinensis)

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