Tea Caffeine synthase (TCS) is one of the key enzymes involved in caffieine biosynthsis in tea plant (Camellia sinensis),which catalyses conversions of 7-methylxanthine to theobromine and theobromine to caffeine. Inhibition of TCS gene expression can leads to breeding low caffeine tea cultivars. Two cDNA fragments of TCS gene were amplified by RT-PCR, and ligated into T-vector. The two TCS gene fragments were inserted into RNAi vector pFGC5941 in reverse direction after double digestion with two pairs of restriction endonucleases. The insertion of two fragments, namely pFGC5941-TCS02 and pFGC5941-TCS03, into the RNAi vector were confirmed by PCR and DNA sequencing.
ZHANG Guang-hui
,
LIANG Yue-rong
,
LU Jian-liang
,
DONG Jun-jie
. Construction of Tea Caffeine Synthase Gene RNAi Vector[J]. Journal of Tea Science, 2006
, 26(4)
: 243
-248
.
DOI: 10.13305/j.cnki.jts.2006.04.002
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