Journal of Tea Science

The Historic Position of Hunan Tea Culture

SHI Zhao-peng

Journal of Tea Science. 2005, 25(1): 1-6. doi:10.13305/j.cnki.jts.2005.01.001

Abstract ( 725 )

PDF (379KB) ( 630 )

References | Related Articles | Metrics | Cite this Article

The historic position of Hunan tea culture was discussed from the historic legends, excavated culture relics and historic records. Some records in the “Tea Classic” by Luyu were texturally investigated by the author. The tea cup come from the Yue Zhou stove(one of six china stoves in Tang dynasty)was the texturally investigated and compared with the Changsha china stove of Tang dynasty. These two china stoves of Tang dynasty in Hunan province created ceramic glaze and decoration which had great scientific value in ancient Chinese china field. Besides, the history of Jun-shan and Bai he-lin tea mentioned in the “Tang State history” was discussed by the author also. The relationship of tea and Zen which has long controversy in tea field was discussed in the paper and agrees with the idea that the original place is the Xia Shan temple of Shi Men in Hunan province written by Yuang Wu Ke Qing in Song dynasty. The important historic role of dark tea in Hunan province in the union of all ethic groups and in the tea economy was put forward by the author.

Construction of AFLP Molecular Markers Linkage Map in Tea Plant

HUANG Jian-an, LI Jia-xian, HUANG Yi-huan, LUO Jun-wu,GONG Zhi-hua, LIU Zhong-hua

Journal of Tea Science. 2005, 25(1): 7-15. doi:10.13305/j.cnki.jts.2005.01.002

Abstract ( 502 )

PDF (962KB) ( 387 )

References | Related Articles | Metrics | Cite this Article

Based on an improved method of AFLP, AFLP markers were employed for construction of a linkage map used a set of 69 F1 progenies of Qi Men No.4×Chao An Da Wu Ye as a mapping population. In this work, all together 1925 bands were obtained by 22 pairs of primers and 87.5 bands each primer pair on average, a total of 485 (25.19%) polymorphic AFLP markers were detected. The 356 (73.40%) of polymorphic markers with Mendelian segregation ratio (P=0.01) were obtained. 247（69.38%）of the 356 loci were 1:1 segregation, 109（30.62%）of the 356 loci were 3:1 segregation. Furthermore, the AFLP molecular markers linkage maps of Qi Men No.4 and Chao An Da Wu Ye were constructed respectively by using Mapmaker/Exp (version3.0). The map of female parent included 17 linkage groups and located 208 markers, covered total map length of 2457.7 cM, and average distance between markers was 11.9 cM. The map of male parent included 16 linkage groups and located 200 markers,covered total map length of 2545.3 cM, and average distance between markers was 12.8 cM.

Purification by Affinity Chromatography and Characterization of Tea Leaf -Glucosidase

ZHANG Zheng-zhu, WAN Xiao-chun, KANZO Sakata

Journal of Tea Science. 2005, 25(1): 16-22. doi:10.13305/j.cnki.jts.2005.01.004

Abstract ( 509 )

PDF (762KB) ( 365 )

References | Related Articles | Metrics | Cite this Article

Two kinds of β-Glucosidases from Camellia sinensis var. sinensis cv. Yabukita were purified by a sophisticate application of affinity chromatography. An affinity adsorbent for β-Glucosidases has been prepared with a positively charged β-glucosylamidine as a ligand in which a glycon moiety is connected via a N-glycoside linkage. The synthesized affinity adsorbent is quite stable under mildly acidic conditions (pH5~6) and can be used up to 20 times over three months without significant affect on the column performance. Two tea leaf β-Glucosidases have been purified to be monomers with molecular mass of 63 and 75 kDa by affinity chromatography at pH6.0 and pH5.0, respectively. The extensive characterization of tea leaf β-Glucosidasse has been reported. Tea aroma precursors such as benzyl, (Z)-3-hexenyl and D-mandelonitrile β-D-glucopyranosides were hydrolyzed by the purified two β-Glucosidases to liberate tea aroma.

An Analysis Method Study of Sequential Extraction and Separation of the Chemical Form of Lead in Tea Garden Soil

SHI Yuan-zhi, KANG Meng-li, MA li-feng, HAN Wen-yan

Journal of Tea Science. 2005, 25(1): 23-29. doi:10.13305/j.cnki.jts.2005.01.005

Abstract ( 402 )

PDF (359KB) ( 303 )

References | Related Articles | Metrics | Cite this Article

With modified sequential extraction and separation analysis method, lead in tea garden was divided into six fractions: water soluble, exchangeable, bound to carbonates, bound to Fe-Mn oxides, bound to organic matter, and residual. All the six forms of lead and total lead were determined with ICP-OES, showing that this method has good precision ,accurate, reliable and easy to operate.

A Study on Screening Germplasm Resources of Tea Plant with High Ester-Catechin Content

LIN Jin-ke, CHEN Rong-bin, CHEN Chang-song, YOU Xiao-mei, ZHANG Ying-gen, CHEN Lin, LI Xiu-feng

Journal of Tea Science. 2005, 25(1): 30-36. doi:10.13305/j.cnki.jts.2005.01.006

Abstract ( 554 )

PDF (418KB) ( 683 )

References | Related Articles | Metrics | Cite this Article

In order to screen special germplasm resources of tea plant (Camellia sinensis (L.) O. Kuntze) with high ester-catechin content, 780 tea plant resources in the Tea Variety Garden of Fujian were utilized as material. According to the appearance of tea plant, 45 tea plant resources were screened as candidate. The ester-catechin contents of spring tea shoots, summer tea shoots and autumn tea shoots in 45 cultivars or lines were analyzed by isocratic elution HPLC. The result was as follows: ester-catechin content of tea plant shoots varied greatly with cultivars or lines. In spring tea shoots, summer tea shoots, autumn tea shoots, and the ester-catechin contents in 45 cultivars or lines were in the range of 5.21%~24.03%、3.05%~20.31%、2.89%~19.66%, averaged 16.08%、14.73%、9.96%, respectively. The variation coefficients were 22.26%、24.10%、40.76%, respectively. The ester-catechin contents in the shoot of the same cultivars in different season, mostly: spring tea shoots >summer tea shoots >autumn tea shoots. There were 5 cultivars and lines(033、505、509、524、1005), which the annual average ester-catechin contents exceeded 19%. These could be considered as special tea plant germplasm resources with high ester-catechin contents and be used as important materials of both quality genetic improvement and the extraction of ester-catechin from tea plant. There were 8 cultivars and lines(033、505、509、510（1）、510（2）、524、1001、1005), which the ratio of ester-catechin contents to total catechins content exceeded 80%（ordinary level is 68%）. These could be used as special materials for extracting the tea polyphenols or catechins.

Study on the Method of Determining Tea Color with Computer

WANG Wen-jie, LUO Shou-jin, HUANG Jian-qin, ZHANG Li-ping, WU Xin-rong, WANG Hong-shu

Journal of Tea Science. 2005, 25(1): 37-42. doi:10.13305/j.cnki.jts.2005.01.007

Abstract ( 425 )

PDF (277KB) ( 215 )

References | Related Articles | Metrics | Cite this Article

Effects of Elevated Atmospheric CO₂ Concentration on Photo-physiological Characteristics of Tea Plant

JIANG Yue-lin, ZHANG Shi-ding, ZHANG Qing-guo

Journal of Tea Science. 2005, 25(1): 43-48. doi:10.13305/j.cnki.jts.2005.01.008

Abstract ( 373 )

PDF (407KB) ( 413 )

References | Related Articles | Metrics | Cite this Article

The net photosynthesis(Pn), transpiration(Tr), stomatal conductance(Sc), water use efficiencyβ(WUE), chlorophyll contents and nutrient elements content of tea plant under ambient and elevated CO₂ conditions were measured, and responses to elevated CO₂ were discussed. The results showed that Pn increased by 17.9% and 25.8% under elevated (550 and 750μmol·mol^-1)CO₂, compared to the ambient air CO₂ concentration. At the same time, midday depression of photosynthesis abated or disappeared. Sc reduced by 7.6% and 13.0% and Tr decreased slightly. WUE increased by 21.6% and 35.8%. It was also found that chlorophyll a, chlorophyll b, total chlorophyll and carotennoid contents increased by 12.8%~18.4%, 14.0%~22.0%, 13.1%~19.4% and 17.2%~20.1% in shoots of tea plant. However, the ratio of chlorophyll a to b reduced. The N, K, and Ca contents in shoots decreased to some extent. The Mg, Fe, Zn, Mn and Cu contents increased with CO₂ enrichment.

The Composition and Chemiluminescence Analysis of Theaflavins in Enzymatic Oxidation Products of Tea Polyphenols

DING Zhao-tang, WANG Xiu-feng, YU Hai-ning, SHEN Sheng-rong

Journal of Tea Science. 2005, 25(1): 49-55. doi:10.13305/j.cnki.jts.2005.01.009

Abstract ( 458 )

PDF (315KB) ( 217 )

References | Related Articles | Metrics | Cite this Article

In this paper, theaflavins extracts from black tea and tea polyphenols were used as control, composition of theaflavins in enzymatic oxidation products was analyzed by HPLC, and the antioxidant activity was investigated by the technique of chemiluminescence. Results showed that, TF_2A was the main composition in enzymatic oxidation products. In theaflavins extracts from black tea, there mainly existed four kinds of theaflavins and their contents were not greatly fluctuated. By employing CuSO₄-Vc-H₂O₂-yeast and Fe²⁺-H₂O₂-yeast systems, antioxidant activity of enzymatic oxidation products was better than theaflavins extracts from black tea and tea polyphenols.

Occurrence of Bacillus thuringiensis on the Phylloplane and Screening of Highly Toxic Strains to Tea Pests

ZHANG Ling-ling, LIN Jin, LUO Lan, FANG Fang, HUANG Tian-pei, XU Jin-han, WU Guang-yuan, WANG Qin-sen, GUAN Xiong

Journal of Tea Science. 2005, 25(1): 56-60. doi:10.13305/j.cnki.jts.2005.01.010

Abstract ( 497 )

PDF (363KB) ( 359 )

References | Related Articles | Metrics | Cite this Article

An isolation method was developed with comparing different selective culture media and 54 Bt strains were isolated from 25 species of plants in China. The bioassay was conducted by use of Ectropis obliqua, Empoasca vitis and Acaphylla theae as target insects. It showed that there were 10 strains effective to Lepidoptera and 4 to Homoptera but they were all ineffective to Acaphylla theae mites.

Effect of Tea Polysaccharides on Immune Functions and Antioxdative Activity in Broilers

HU Zhong-ze, JIN Guang-ming, WANG Li-ke, YANG Jiu-feng

Journal of Tea Science. 2005, 25(1): 61-64. doi:10.13305/j.cnki.jts.2005.01.011

Abstract ( 416 )

PDF (312KB) ( 198 )

References | Related Articles | Metrics | Cite this Article

To study the effect of tea polysaccharide on immune function and antioxidative activity in broilers, 0%、0.2%、0.4% of tea polysaccharide were added into the drinking water for broilers respectively. Results showed that thymus index and serum IgG levels of broilers were increased obviously by TP（P<0.05）, furthermore lymphocyte transformation rate, macrophage activity, percentage of T cell numbers Serum SOD, GSH-Px and CAT activities in broilers were increased obviously by TP at same time（P<0.05）but not to index of fabricius bursa of broilers. Serum MDA levels of broilers were declined obviously by TP（P<0.05）.

Analysis of AcORF47 Homologue and VP80 Gene from a Newly Isolated Nucleopolyhedrovirus from the Tea Slug Moth, Iragoides fasciata

YANG Li-rong, XIAO Qiang, LENG Yang, XU Hai-jun, ZHANG Yi-qing, ZHANG Chuan-Xi

Journal of Tea Science. 2005, 25(1): 65-70. doi:10.13305/j.cnki.jts.2005.01.012

Abstract ( 509 )

PDF (318KB) ( 274 )

References | Related Articles | Metrics | Cite this Article

A baculovirus, Iragoides fasciata Nucleopolyhedrovirus（IrfaNPV）was newly isolated from the tea slug moth, Iragoides fasciata. The IrfaNPV AcORF47 homologue and VP80 gene were cloned. Sequence analysis showed IrfaNPV AcORF47 homologue shared 42%, 42% and 40% identities with AcMNPV ORF47, BmNPV ORF38 and RaouMNPV ORF44 in amino acid level, respectively. The 222 amino acid residues in the C-terminal of IrfaNPV VP80 had 25%~58% identities with homologous genes from other 17 NPVs. A phylogenetic tree was constructed based on the VP80/P87 amino acid sequences and the result showed that IrfaNPV was most closely related to AcMNPV, BmNPV and RaouMNPV.

Synthesis of tea polysaccharide-Fe（Ⅲ）complex and determination of Fe（Ⅲ）

LI Yu-xian, PEI Xiao-hong

Journal of Tea Science. 2005, 25(1): 71-74. doi:10.13305/j.cnki.jts.2005.01.013

Abstract ( 400 )

PDF (317KB) ( 220 )

References | Related Articles | Metrics | Cite this Article

Tea polysaccharide was abstracted with warm water, tea polysaccharide-Fe（Ⅲ）complex(TPC) was synthesized with tea polysaccharide and FeCl₃ under the alkaline condition, The determination of Fe（Ⅲ） was conducted with the method of ferroin-Fe（Ⅱ） and atomic absorption spectrophometry. The results showed that: TPC is heavy brownish red and shapeless powder. It can dissolve in water, its solution is very stable at the pH from 3 to 12. Content of Fe（Ⅲ）in TPC is 20.71% by the method of ferroin- Fe（Ⅱ）and 20.13% by the method of atomic absorption.

Studies on the Fluorescent Green Spot Disease on Tea Foliage

ZHANG Li-xia, GUO Yan-kui, HUANG Xiao-qin, XIANG Qin-zeng, XIA Wen-juan, JIA Ming

Journal of Tea Science. 2005, 25(1): 75-80. doi:10.13305/j.cnki.jts.2005.01.014

Abstract ( 500 )

PDF (926KB) ( 286 )

References | Related Articles | Metrics | Cite this Article

By using the methods of field investigation, microbial cultivation, pathological anatomy and electron probe, a preliminary investigation on the symptom, characteristic of occurrence and field distribution, etiology of the fluorescent green spot disease on tea leaves was conducted by the authors. Results showed that the representative symptom of this disease was green spots with fluorescence and irregular in shape; the lower epidermis of diseased leaves showed local convex which caused by the pathological changes of the spongy cells including the structural damage, volume expanding and the extrusion between the spongy cells. The occurrence of disease was closely related with the growth vigour of tea plant and nutrient supply. The distribution of disease in tea garden was characteristic of scattering and asymmetric, and having no disease center of occurrence. Pathogenic microbes weren’t discovered in the process of microbial isolation and cultivation. However, by transmission electron microscope, it could be observed that the cell membranes were damaged and many vacuoles formed in cytoplasm. By scanning electron microscope, various abnormal cells were found in tea foliage at the granular protrusions. Meanwhile, a certain amounts of calcium oxalate crystals were found within the spongy cells. On the basis of all above results, it could be deduced that this disease was classified to the physiological types．

Table of Content